Lactic acid production by Carnobacterium sp. isolated from a maritime Antarctic lake using eucalyptus enzymatic hydrolysate

• Published in: Biotechnology Reports
• Main Authors: Laura Camesasca, Juan Andrés de Mattos, Eugenia Vila, Florencia Cebreiros, Claudia Lareo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2021
• Subjects: Carnobacterium; Lactic acid; Lignocellulose; Eucalyptus; Fermentation; Biotechnology; TP248.13-248.65; Antarctic; Antarc*
• Online Access: https://doi.org/10.1016/j.btre.2021.e00643; https://doaj.org/article/2ba41614e01d427db5172c0ba7f843ad

Carnobacterium sp., a lactic acid bacterium isolated from a maritime Antarctic lake, was evaluated for lactic acid production from a lignocellulosic hydrolysate. Eucalyptus sawdust, a residue from pulp and paper industries, was subjected to alkaline pretreatment to enhance its enzymatic hydrolysis. Fermentations were performed without and with pH control using eucalyptus enzymatic hydrolysate containing a mixture of glucose and xylose sugars. The sugars were successfully converted into lactic acid in 24 h, resulting in 7.6 g/L of lactic acid and a product yield of 0.50 g/g for pH controlled at 6.5. Fed-batch fermentation performed at a controlled pH of 6.5 improved both the lactic acid production (30 g/L) and the biomass growth (4.2 g/L). l-lactic acid optical purity higher than 95 % was obtained. These results demonstrated the potential usage of Carnobacterium sp in l-lactic acid production from eucalyptus.