Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better under...
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2020
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Online Access: | https://doi.org/10.3390/md18060298 https://doaj.org/article/2b8a2c2fe5d3479582a5a8baf73e92d3 |
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ftdoajarticles:oai:doaj.org/article:2b8a2c2fe5d3479582a5a8baf73e92d3 2023-05-15T14:04:22+02:00 Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker 2020-06-01T00:00:00Z https://doi.org/10.3390/md18060298 https://doaj.org/article/2b8a2c2fe5d3479582a5a8baf73e92d3 EN eng MDPI AG https://www.mdpi.com/1660-3397/18/6/298 https://doaj.org/toc/1660-3397 doi:10.3390/md18060298 1660-3397 https://doaj.org/article/2b8a2c2fe5d3479582a5a8baf73e92d3 Marine Drugs, Vol 18, Iss 298, p 298 (2020) Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/md18060298 2022-12-30T20:28:47Z Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum , in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64° 46'S, 64° 03'W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Antarctic Peninsula Anvers ENVELOPE(-63.500,-63.500,-64.600,-64.600) Anvers Island ENVELOPE(-63.500,-63.500,-64.600,-64.600) Pala ENVELOPE(40.637,40.637,64.896,64.896) Marine Drugs 18 6 298 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Biology (General) QH301-705.5 |
spellingShingle |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Biology (General) QH301-705.5 Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
topic_facet |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Biology (General) QH301-705.5 |
description |
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum , in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64° 46'S, 64° 03'W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. |
format |
Article in Journal/Newspaper |
author |
Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker |
author_facet |
Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker |
author_sort |
Alison E. Murray |
title |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
title_short |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
title_full |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
title_fullStr |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
title_full_unstemmed |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago , Antarctica |
title_sort |
uncovering the core microbiome and distribution of palmerolide in synoicum adareanum across the anvers island archipelago , antarctica |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18060298 https://doaj.org/article/2b8a2c2fe5d3479582a5a8baf73e92d3 |
long_lat |
ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(40.637,40.637,64.896,64.896) |
geographic |
Antarctic The Antarctic Antarctic Peninsula Anvers Anvers Island Pala |
geographic_facet |
Antarctic The Antarctic Antarctic Peninsula Anvers Anvers Island Pala |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
op_source |
Marine Drugs, Vol 18, Iss 298, p 298 (2020) |
op_relation |
https://www.mdpi.com/1660-3397/18/6/298 https://doaj.org/toc/1660-3397 doi:10.3390/md18060298 1660-3397 https://doaj.org/article/2b8a2c2fe5d3479582a5a8baf73e92d3 |
op_doi |
https://doi.org/10.3390/md18060298 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
6 |
container_start_page |
298 |
_version_ |
1766275408519495680 |