Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran
The current study aimed to evaluate the seroprevalence of visceral leishmaniasis in asymptomatic healthy children in a rural area of Fars province, Southern Iran. Blood samples were taken from 617 asymptomatic healthy children and serum samples along with buffy coat were separated from the blood. Th...
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ftdoajarticles:oai:doaj.org/article:2b19bddcbce346dc9fb267ecaded13f2 2024-09-09T19:26:22+00:00 Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran Akram Layegh Gigloo Bahador Sarkari Zahra Rezaei Gholam Reza Hatam Mohammad Hassan Davami 2018-01-01T00:00:00Z https://doi.org/10.1155/2018/8167247 https://doaj.org/article/2b19bddcbce346dc9fb267ecaded13f2 EN eng Wiley http://dx.doi.org/10.1155/2018/8167247 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8167247 https://doaj.org/article/2b19bddcbce346dc9fb267ecaded13f2 Journal of Tropical Medicine, Vol 2018 (2018) Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.1155/2018/8167247 2024-08-05T17:48:37Z The current study aimed to evaluate the seroprevalence of visceral leishmaniasis in asymptomatic healthy children in a rural area of Fars province, Southern Iran. Blood samples were taken from 617 asymptomatic healthy children and serum samples along with buffy coat were separated from the blood. The serum samples were assessed for antibodies against Leishmania infantum by an indirect ELISA and the buffy coats were tested for the presence of L. infantum DNA by molecular method. Of the 617 recruited children, 297 (48.1%) were female and 317 (51.4%) were male. Anti-Leishmania antibodies were detected in 17 (2.8%) of the children. From those 17 seropositive cases, 5 (29.4%) were male and 12 (70.6%) cases were female. Children aged 5–8 years had the highest seroprevalence rate; however, no associations were found between seropositivity to Leishmania and gender or age of the children. Moreover, L. infantum DNA was detected in buffy coat of 8 (1.3%) of 617 children. Three of the PCR-positive cases were seropositive whereas 14 of seropositive subjects (82.3%) were PCR-negative. Findings of the current study revealed a considerable subclinical leishmanial infection in children in the studied rural area in the south of Iran. Results of the current study could be used for surveillance, prevention, and control of VL in the area. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2018 1 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Akram Layegh Gigloo Bahador Sarkari Zahra Rezaei Gholam Reza Hatam Mohammad Hassan Davami Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
The current study aimed to evaluate the seroprevalence of visceral leishmaniasis in asymptomatic healthy children in a rural area of Fars province, Southern Iran. Blood samples were taken from 617 asymptomatic healthy children and serum samples along with buffy coat were separated from the blood. The serum samples were assessed for antibodies against Leishmania infantum by an indirect ELISA and the buffy coats were tested for the presence of L. infantum DNA by molecular method. Of the 617 recruited children, 297 (48.1%) were female and 317 (51.4%) were male. Anti-Leishmania antibodies were detected in 17 (2.8%) of the children. From those 17 seropositive cases, 5 (29.4%) were male and 12 (70.6%) cases were female. Children aged 5–8 years had the highest seroprevalence rate; however, no associations were found between seropositivity to Leishmania and gender or age of the children. Moreover, L. infantum DNA was detected in buffy coat of 8 (1.3%) of 617 children. Three of the PCR-positive cases were seropositive whereas 14 of seropositive subjects (82.3%) were PCR-negative. Findings of the current study revealed a considerable subclinical leishmanial infection in children in the studied rural area in the south of Iran. Results of the current study could be used for surveillance, prevention, and control of VL in the area. |
format |
Article in Journal/Newspaper |
author |
Akram Layegh Gigloo Bahador Sarkari Zahra Rezaei Gholam Reza Hatam Mohammad Hassan Davami |
author_facet |
Akram Layegh Gigloo Bahador Sarkari Zahra Rezaei Gholam Reza Hatam Mohammad Hassan Davami |
author_sort |
Akram Layegh Gigloo |
title |
Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
title_short |
Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
title_full |
Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
title_fullStr |
Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
title_full_unstemmed |
Asymptomatic Leishmania Infected Children: A Seroprevalence and Molecular Survey in a Rural Area of Fars Province, Southern Iran |
title_sort |
asymptomatic leishmania infected children: a seroprevalence and molecular survey in a rural area of fars province, southern iran |
publisher |
Wiley |
publishDate |
2018 |
url |
https://doi.org/10.1155/2018/8167247 https://doaj.org/article/2b19bddcbce346dc9fb267ecaded13f2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2018 (2018) |
op_relation |
http://dx.doi.org/10.1155/2018/8167247 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8167247 https://doaj.org/article/2b19bddcbce346dc9fb267ecaded13f2 |
op_doi |
https://doi.org/10.1155/2018/8167247 |
container_title |
Journal of Tropical Medicine |
container_volume |
2018 |
container_start_page |
1 |
op_container_end_page |
6 |
_version_ |
1809895995140472832 |