Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection
Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned pr...
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ftdoajarticles:oai:doaj.org/article:2b0b05d466df4de08ad7b6a0aabe813f 2023-05-15T15:18:38+02:00 Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection Mellina T. Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E. Selkirk Tracey J. Lamb David B. Guiliano David Joseph Diemert 2020-11-01T00:00:00Z https://doaj.org/article/2b0b05d466df4de08ad7b6a0aabe813f EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673540/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/2b0b05d466df4de08ad7b6a0aabe813f PLoS Neglected Tropical Diseases, Vol 14, Iss 11 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles 2022-12-31T14:00:48Z Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned proteins have shown that some ESPs are potent immunogens capable of eliciting protective immune responses. Here we describe two novel proteins, Secreted from Muscle stage Larvae SML-4 and SML-5 which are 15 kDa and 12 kDa respectively. The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. Vaccination with these proteins resulted in an impaired establishment of adult stages and consequently a reduction in the burden of mL1 in BALB/c mice. This suggests that both proteins may be important for establishment of parasite infection of the intestine and are prophylactic vaccine candidates. Author summary Author summary Trichinellosis, also known as trichinosis, is a disease caused by consuming raw or undercooked meat from animals infected with muscle stage larvae of Trichinella spiralis. These worms cause infection when L1 (newborn) larvae migrate and invade skeletal muscle remodeling muscle cells into long-lived nurse cells which serve as reservoirs for the parasite. The establishment of nurse cells allows T. spiralis to better survive within the host because factors, such as blood vessel formation, bring required components for survival to the worms. We previously found three novel proteins termed Secreted from Muscle stage Larvae (SML)-1, -2, and -3 by transcriptomic analysis. In this study, we further expand our previous findings by identifying two novel low molecular weight proteins SML-4 and SML-5, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mellina T. Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E. Selkirk Tracey J. Lamb David B. Guiliano David Joseph Diemert Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichinella spiralis muscle stage larvae (mL1) produce excretory-secreted products (ESPs), a complex mixture of protein, which are believed to be important for establishing or maintaining an infection niche within skeletal muscle and the intestine. Studies of both whole ESPs and individual cloned proteins have shown that some ESPs are potent immunogens capable of eliciting protective immune responses. Here we describe two novel proteins, Secreted from Muscle stage Larvae SML-4 and SML-5 which are 15 kDa and 12 kDa respectively. The genes encoding these proteins are highly conserved within the Trichinellids, are constituents of mL1 ESP and localized in the parasite stichosome. While SML-5 is only expressed in mL1 and early stages of adult nematode development, SML-4 is a tyvosylated glycoprotein also produced by adult nematodes, indicating it may have a function in the enteral phase of the infection. Vaccination with these proteins resulted in an impaired establishment of adult stages and consequently a reduction in the burden of mL1 in BALB/c mice. This suggests that both proteins may be important for establishment of parasite infection of the intestine and are prophylactic vaccine candidates. Author summary Author summary Trichinellosis, also known as trichinosis, is a disease caused by consuming raw or undercooked meat from animals infected with muscle stage larvae of Trichinella spiralis. These worms cause infection when L1 (newborn) larvae migrate and invade skeletal muscle remodeling muscle cells into long-lived nurse cells which serve as reservoirs for the parasite. The establishment of nurse cells allows T. spiralis to better survive within the host because factors, such as blood vessel formation, bring required components for survival to the worms. We previously found three novel proteins termed Secreted from Muscle stage Larvae (SML)-1, -2, and -3 by transcriptomic analysis. In this study, we further expand our previous findings by identifying two novel low molecular weight proteins SML-4 and SML-5, ... |
format |
Article in Journal/Newspaper |
author |
Mellina T. Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E. Selkirk Tracey J. Lamb David B. Guiliano David Joseph Diemert |
author_facet |
Mellina T. Srey Alessia Taccogna Yelena Oksov Sara Lustigman Pei-Yi Tai John Acord Murray E. Selkirk Tracey J. Lamb David B. Guiliano David Joseph Diemert |
author_sort |
Mellina T. Srey |
title |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
title_short |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
title_full |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
title_fullStr |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
title_full_unstemmed |
Vaccination with novel low-molecular weight proteins secreted from Trichinella spiralis inhibits establishment of infection |
title_sort |
vaccination with novel low-molecular weight proteins secreted from trichinella spiralis inhibits establishment of infection |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doaj.org/article/2b0b05d466df4de08ad7b6a0aabe813f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11 (2020) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673540/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/2b0b05d466df4de08ad7b6a0aabe813f |
_version_ |
1766348820537409536 |