Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa?
Abstract The intensification of malaria control interventions has resulted in its global decline, but it remains a significant public health burden especially in sub-Saharan Africa (sSA). Knowledge on the parasite diversity, its transmission dynamics, mechanisms of adaptation to environmental and in...
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ftdoajarticles:oai:doaj.org/article:2afd592f11464afd8bdd334274516c5a 2023-05-15T15:13:44+02:00 Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? Tobias O. Apinjoh Amed Ouattara Vincent P. K. Titanji Abdoulaye Djimde Alfred Amambua-Ngwa 2019-06-01T00:00:00Z https://doi.org/10.1186/s12936-019-2844-5 https://doaj.org/article/2afd592f11464afd8bdd334274516c5a EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2844-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2844-5 1475-2875 https://doaj.org/article/2afd592f11464afd8bdd334274516c5a Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) Genetic diversity Drug resistance Monitoring Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2844-5 2022-12-31T11:30:55Z Abstract The intensification of malaria control interventions has resulted in its global decline, but it remains a significant public health burden especially in sub-Saharan Africa (sSA). Knowledge on the parasite diversity, its transmission dynamics, mechanisms of adaptation to environmental and interventional pressures could help refine or develop new control and elimination strategies. Critical to this is the accurate assessment of the parasite’s genetic diversity and monitoring of genetic markers of anti-malarial resistance across all susceptible populations. Such wide molecular surveillance will require selected tools and approaches from a variety of ever evolving advancements in technology and the changing epidemiology of malaria. The choice of an effective approach for specific endemic settings remains challenging, particularly for countries in sSA with limited access to advanced technologies. This article examines the current strategies and tools for Plasmodium falciparum genetic diversity typing and resistance monitoring and proposes how the different tools could be employed in resource-poor settings. Advanced approaches enabling targeted deep sequencing is valued as a sensitive method for assessing drug resistance and parasite diversity but remains out of the reach of most laboratories in sSA due to the high cost of development and maintenance. It is, however, feasible to equip a limited number of laboratories as Centres of Excellence in Africa (CEA), which will receive and process samples from a network of peripheral laboratories in the continent. Cheaper, sensitive and portable real-time PCR methods can be used in peripheral laboratories to pre-screen and select samples for targeted deep sequence or genome wide analyses at these CEAs. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Genetic diversity Drug resistance Monitoring Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Genetic diversity Drug resistance Monitoring Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Tobias O. Apinjoh Amed Ouattara Vincent P. K. Titanji Abdoulaye Djimde Alfred Amambua-Ngwa Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
topic_facet |
Genetic diversity Drug resistance Monitoring Plasmodium falciparum Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract The intensification of malaria control interventions has resulted in its global decline, but it remains a significant public health burden especially in sub-Saharan Africa (sSA). Knowledge on the parasite diversity, its transmission dynamics, mechanisms of adaptation to environmental and interventional pressures could help refine or develop new control and elimination strategies. Critical to this is the accurate assessment of the parasite’s genetic diversity and monitoring of genetic markers of anti-malarial resistance across all susceptible populations. Such wide molecular surveillance will require selected tools and approaches from a variety of ever evolving advancements in technology and the changing epidemiology of malaria. The choice of an effective approach for specific endemic settings remains challenging, particularly for countries in sSA with limited access to advanced technologies. This article examines the current strategies and tools for Plasmodium falciparum genetic diversity typing and resistance monitoring and proposes how the different tools could be employed in resource-poor settings. Advanced approaches enabling targeted deep sequencing is valued as a sensitive method for assessing drug resistance and parasite diversity but remains out of the reach of most laboratories in sSA due to the high cost of development and maintenance. It is, however, feasible to equip a limited number of laboratories as Centres of Excellence in Africa (CEA), which will receive and process samples from a network of peripheral laboratories in the continent. Cheaper, sensitive and portable real-time PCR methods can be used in peripheral laboratories to pre-screen and select samples for targeted deep sequence or genome wide analyses at these CEAs. |
format |
Article in Journal/Newspaper |
author |
Tobias O. Apinjoh Amed Ouattara Vincent P. K. Titanji Abdoulaye Djimde Alfred Amambua-Ngwa |
author_facet |
Tobias O. Apinjoh Amed Ouattara Vincent P. K. Titanji Abdoulaye Djimde Alfred Amambua-Ngwa |
author_sort |
Tobias O. Apinjoh |
title |
Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
title_short |
Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
title_full |
Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
title_fullStr |
Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
title_full_unstemmed |
Genetic diversity and drug resistance surveillance of Plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-Saharan Africa? |
title_sort |
genetic diversity and drug resistance surveillance of plasmodium falciparum for malaria elimination: is there an ideal tool for resource-limited sub-saharan africa? |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2844-5 https://doaj.org/article/2afd592f11464afd8bdd334274516c5a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-12 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2844-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2844-5 1475-2875 https://doaj.org/article/2afd592f11464afd8bdd334274516c5a |
op_doi |
https://doi.org/10.1186/s12936-019-2844-5 |
container_title |
Malaria Journal |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766344260763779072 |