The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran
Abstract Background Baylisascaris devosi is an intestinal nematode found in several carnivores including fisher, wolverine, Beech marten, American marten and sable in different parts of the world, but this nematode has not been reported from Pine marten. Therefore, this study aimed to identify Bayli...
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ftdoajarticles:oai:doaj.org/article:2adf1d0dee114f63ab3666c550839386 2023-05-15T13:21:52+02:00 The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran Meysam Sharifdini Richard A. Heckmann Fattaneh Mikaeili 2021-01-01T00:00:00Z https://doi.org/10.1186/s13071-020-04513-4 https://doaj.org/article/2adf1d0dee114f63ab3666c550839386 EN eng BMC https://doi.org/10.1186/s13071-020-04513-4 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04513-4 1756-3305 https://doaj.org/article/2adf1d0dee114f63ab3666c550839386 Parasites & Vectors, Vol 14, Iss 1, Pp 1-14 (2021) Baylisascaris devosi Martes martes Iran Molecular characterization SEM EDAX Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s13071-020-04513-4 2022-12-31T04:43:42Z Abstract Background Baylisascaris devosi is an intestinal nematode found in several carnivores including fisher, wolverine, Beech marten, American marten and sable in different parts of the world, but this nematode has not been reported from Pine marten. Therefore, this study aimed to identify Baylisascaris isolated from a Pine marten in Iran using morphological and molecular approaches. Methods Specimens of B. devosi were collected from one road-killed Pine marten in northern Iran. Morphological features were evaluated using scanning electron microscopy, energy dispersive x-ray analysis and ion sectioning. The molecular characterization was carried out using partial Cox1, LSU rDNA and ITS-rDNA genes. Results The nematodes isolated from the Pine marten were confirmed to be B. devosi based on the morphological features and the sequence of ribosomal and mitochondrial loci. X-ray scans (EDAX) were completed on gallium cut structures (papillae, eggs, male spike and mouth denticles) of B. devosi using a dual-beam scanning electron microscope. The male spike and mouth denticles had a high level of hardening elements (Ca, P, S), helping to explain the chemical nature and morphology of the worm. Based on these genetic marker analyses, our sequence had the greatest similarity with Russian B. devosi isolated from sable. Conclusions In this study, to our knowledge, the occurrence of B. devosi infection in Pine marten is reported for the first time. Molecular analysis showed that these three genes are suitable molecular markers for identification and inferring phylogenetic relationships of Baylisascaris species. Furthermore, the high divergence of Cox1 between Baylisascaris species indicates that Cox1 could be used for their phylogenetic and taxonomic studies. Article in Journal/Newspaper American marten Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 14 1 |
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topic |
Baylisascaris devosi Martes martes Iran Molecular characterization SEM EDAX Infectious and parasitic diseases RC109-216 |
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Baylisascaris devosi Martes martes Iran Molecular characterization SEM EDAX Infectious and parasitic diseases RC109-216 Meysam Sharifdini Richard A. Heckmann Fattaneh Mikaeili The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
topic_facet |
Baylisascaris devosi Martes martes Iran Molecular characterization SEM EDAX Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Baylisascaris devosi is an intestinal nematode found in several carnivores including fisher, wolverine, Beech marten, American marten and sable in different parts of the world, but this nematode has not been reported from Pine marten. Therefore, this study aimed to identify Baylisascaris isolated from a Pine marten in Iran using morphological and molecular approaches. Methods Specimens of B. devosi were collected from one road-killed Pine marten in northern Iran. Morphological features were evaluated using scanning electron microscopy, energy dispersive x-ray analysis and ion sectioning. The molecular characterization was carried out using partial Cox1, LSU rDNA and ITS-rDNA genes. Results The nematodes isolated from the Pine marten were confirmed to be B. devosi based on the morphological features and the sequence of ribosomal and mitochondrial loci. X-ray scans (EDAX) were completed on gallium cut structures (papillae, eggs, male spike and mouth denticles) of B. devosi using a dual-beam scanning electron microscope. The male spike and mouth denticles had a high level of hardening elements (Ca, P, S), helping to explain the chemical nature and morphology of the worm. Based on these genetic marker analyses, our sequence had the greatest similarity with Russian B. devosi isolated from sable. Conclusions In this study, to our knowledge, the occurrence of B. devosi infection in Pine marten is reported for the first time. Molecular analysis showed that these three genes are suitable molecular markers for identification and inferring phylogenetic relationships of Baylisascaris species. Furthermore, the high divergence of Cox1 between Baylisascaris species indicates that Cox1 could be used for their phylogenetic and taxonomic studies. |
format |
Article in Journal/Newspaper |
author |
Meysam Sharifdini Richard A. Heckmann Fattaneh Mikaeili |
author_facet |
Meysam Sharifdini Richard A. Heckmann Fattaneh Mikaeili |
author_sort |
Meysam Sharifdini |
title |
The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
title_short |
The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
title_full |
The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
title_fullStr |
The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
title_full_unstemmed |
The morphological and molecular characterization of Baylisascaris devosi Sprent, 1952 (Ascaridoidea, Nematoda), collected from Pine marten (Martes martes) in Iran |
title_sort |
morphological and molecular characterization of baylisascaris devosi sprent, 1952 (ascaridoidea, nematoda), collected from pine marten (martes martes) in iran |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s13071-020-04513-4 https://doaj.org/article/2adf1d0dee114f63ab3666c550839386 |
genre |
American marten |
genre_facet |
American marten |
op_source |
Parasites & Vectors, Vol 14, Iss 1, Pp 1-14 (2021) |
op_relation |
https://doi.org/10.1186/s13071-020-04513-4 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-020-04513-4 1756-3305 https://doaj.org/article/2adf1d0dee114f63ab3666c550839386 |
op_doi |
https://doi.org/10.1186/s13071-020-04513-4 |
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Parasites & Vectors |
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14 |
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