Filter paper performance in PCR for cutaneous leishmaniasis diagnosis
Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESU...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Online Access: | https://doi.org/10.1590/0037-8682-0047-2020 https://doaj.org/article/2ab4fa256d21485ab825c2fa5ef6f922 |
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ftdoajarticles:oai:doaj.org/article:2ab4fa256d21485ab825c2fa5ef6f922 2023-05-15T15:00:15+02:00 Filter paper performance in PCR for cutaneous leishmaniasis diagnosis Camila Alves Mota Eneide Aparecida Sabaini Venazzi Paulo Donizeti Zanzarini Sandra Mara Alessi Aristides Maria Valdrinez Campana Lonardoni Thaís Gomes Verzignassi Silveira 2020-12-01T00:00:00Z https://doi.org/10.1590/0037-8682-0047-2020 https://doaj.org/article/2ab4fa256d21485ab825c2fa5ef6f922 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100605&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0047-2020 https://doaj.org/article/2ab4fa256d21485ab825c2fa5ef6f922 Revista da Sociedade Brasileira de Medicina Tropical, Vol 54 (2020) Leishmania Cutaneous leishmaniasis Neglected diseases Polymerase chain reaction Diagnosis Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.1590/0037-8682-0047-2020 2022-12-31T02:47:39Z Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULTS: PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%. CONCLUSIONS: FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 54 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Leishmania Cutaneous leishmaniasis Neglected diseases Polymerase chain reaction Diagnosis Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Leishmania Cutaneous leishmaniasis Neglected diseases Polymerase chain reaction Diagnosis Arctic medicine. Tropical medicine RC955-962 Camila Alves Mota Eneide Aparecida Sabaini Venazzi Paulo Donizeti Zanzarini Sandra Mara Alessi Aristides Maria Valdrinez Campana Lonardoni Thaís Gomes Verzignassi Silveira Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
topic_facet |
Leishmania Cutaneous leishmaniasis Neglected diseases Polymerase chain reaction Diagnosis Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULTS: PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%. CONCLUSIONS: FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR. |
format |
Article in Journal/Newspaper |
author |
Camila Alves Mota Eneide Aparecida Sabaini Venazzi Paulo Donizeti Zanzarini Sandra Mara Alessi Aristides Maria Valdrinez Campana Lonardoni Thaís Gomes Verzignassi Silveira |
author_facet |
Camila Alves Mota Eneide Aparecida Sabaini Venazzi Paulo Donizeti Zanzarini Sandra Mara Alessi Aristides Maria Valdrinez Campana Lonardoni Thaís Gomes Verzignassi Silveira |
author_sort |
Camila Alves Mota |
title |
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
title_short |
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
title_full |
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
title_fullStr |
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
title_full_unstemmed |
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis |
title_sort |
filter paper performance in pcr for cutaneous leishmaniasis diagnosis |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2020 |
url |
https://doi.org/10.1590/0037-8682-0047-2020 https://doaj.org/article/2ab4fa256d21485ab825c2fa5ef6f922 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 54 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822021000100605&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0047-2020 https://doaj.org/article/2ab4fa256d21485ab825c2fa5ef6f922 |
op_doi |
https://doi.org/10.1590/0037-8682-0047-2020 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
54 |
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