Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities
Objective: To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions. Methods: The effects of methanolic e...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:2a686ec137e34680ae4f48b45a24fe23 2023-05-15T15:07:00+02:00 Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities Shaymaa Fadhel Abbas Albaayit Abdullah Rasedee Noorlidah Abdullah Yusuf Abba 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.281467 https://doaj.org/article/2a686ec137e34680ae4f48b45a24fe23 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=5;spage=232;epage=238;aulast=Albaayit https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.281467 https://doaj.org/article/2a686ec137e34680ae4f48b45a24fe23 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 5, Pp 232-238 (2020) clausena excavata leaves anti-inflammatory cytokines cytotoxicity apoptotic genes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.281467 2022-12-31T12:03:34Z Objective: To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions. Methods: The effects of methanolic extract of Clausena excavata on in vitro viability and TNF-α, IL-6, IL-10, and nitric oxide release by LPS-activated J774A.1 cells were determined. In addition, relative expressions of BAX, BCL-2 and COX-2 genes were examined in healed wounds of rats. Results: The methanolic extract of Clausena excavata was not toxic to J774A.1 cells at the highest dose of 400 μg/mL. It decreased levels of TNF-α and IL-6, while increasing IL-10 level in LPS- activated J774A.1 cells and in the healed wounds of rats. The methanolic extract of Clausena excavata also inhibited nitric oxide production in LPS-activated J774A.1 cells. The BAX and COX-2 genes were downregulated while the BCL-2 gene was upregulated in the healed wound of rats. Conclusions: The methanolic extract of Clausena excavata promotes wound healing via its anti-inflammatory and anti-apoptotic activities. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 5 232 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
clausena excavata leaves anti-inflammatory cytokines cytotoxicity apoptotic genes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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clausena excavata leaves anti-inflammatory cytokines cytotoxicity apoptotic genes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Shaymaa Fadhel Abbas Albaayit Abdullah Rasedee Noorlidah Abdullah Yusuf Abba Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
topic_facet |
clausena excavata leaves anti-inflammatory cytokines cytotoxicity apoptotic genes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions. Methods: The effects of methanolic extract of Clausena excavata on in vitro viability and TNF-α, IL-6, IL-10, and nitric oxide release by LPS-activated J774A.1 cells were determined. In addition, relative expressions of BAX, BCL-2 and COX-2 genes were examined in healed wounds of rats. Results: The methanolic extract of Clausena excavata was not toxic to J774A.1 cells at the highest dose of 400 μg/mL. It decreased levels of TNF-α and IL-6, while increasing IL-10 level in LPS- activated J774A.1 cells and in the healed wounds of rats. The methanolic extract of Clausena excavata also inhibited nitric oxide production in LPS-activated J774A.1 cells. The BAX and COX-2 genes were downregulated while the BCL-2 gene was upregulated in the healed wound of rats. Conclusions: The methanolic extract of Clausena excavata promotes wound healing via its anti-inflammatory and anti-apoptotic activities. |
format |
Article in Journal/Newspaper |
author |
Shaymaa Fadhel Abbas Albaayit Abdullah Rasedee Noorlidah Abdullah Yusuf Abba |
author_facet |
Shaymaa Fadhel Abbas Albaayit Abdullah Rasedee Noorlidah Abdullah Yusuf Abba |
author_sort |
Shaymaa Fadhel Abbas Albaayit |
title |
Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
title_short |
Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
title_full |
Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
title_fullStr |
Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
title_full_unstemmed |
Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
title_sort |
methanolic extract of clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.281467 https://doaj.org/article/2a686ec137e34680ae4f48b45a24fe23 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 5, Pp 232-238 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=5;spage=232;epage=238;aulast=Albaayit https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.281467 https://doaj.org/article/2a686ec137e34680ae4f48b45a24fe23 |
op_doi |
https://doi.org/10.4103/2221-1691.281467 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
5 |
container_start_page |
232 |
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1766338578985517056 |