Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar)
Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of th...
Published in: | Animal Nutrition |
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KeAi Communications Co., Ltd.
2022
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Online Access: | https://doi.org/10.1016/j.aninu.2022.04.003 https://doaj.org/article/2a2b9da6b9924a0ebfdae0928d8a48a2 |
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ftdoajarticles:oai:doaj.org/article:2a2b9da6b9924a0ebfdae0928d8a48a2 2023-05-15T15:30:25+02:00 Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) Alejandro Villasante Carolina Ramírez Héctor Rodríguez Patricio Dantagnan Adrián Hernández Elías Figueroa Jaime Romero 2022-09-01T00:00:00Z https://doi.org/10.1016/j.aninu.2022.04.003 https://doaj.org/article/2a2b9da6b9924a0ebfdae0928d8a48a2 EN eng KeAi Communications Co., Ltd. http://www.sciencedirect.com/science/article/pii/S2405654522000427 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2022.04.003 https://doaj.org/article/2a2b9da6b9924a0ebfdae0928d8a48a2 Animal Nutrition, Vol 10, Iss , Pp 261-279 (2022) Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Animal culture SF1-1100 article 2022 ftdoajarticles https://doi.org/10.1016/j.aninu.2022.04.003 2022-12-30T19:59:00Z Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times (i.e., weeks 4, 8 and 12), during a 12-week trial. A low carbohydrate-to-high protein diet (LC/HP, 0% wheat starch), a medium carbohydrate-to-medium protein diet (MC/MP, 15% wheat starch) or a high carbohydrate-to-low protein diet (HC/LP, 30% wheat starch) was fed to triplicate fish tanks (27 to 28 fish per tank). We performed an in-depth characterization of the distal intestine digesta microbiota. Further, growth parameters, liver histology and the expression of genes involved in hepatic neolipogenesis in fish were measured. Fish fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes (P = 0.026 and P = 0.018, respectively), lower final weight (P = 0.005), weight gain (P = 0.003), feed efficiency (P = 0.033) and growth rate (P = 0.003) compared with fish fed the LC/HP diet. Further, feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization, steatosis index (P = 0.007) and expression of fatty acid synthase (fas) and delta-6 fatty acyl desaturase (d6fad) (P = 0.001 and P = 0.001, respectively) in the liver compared with fish fed the LC/HP diet. Although, the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4 (HC/LP vs MC/MP and HC/LP vs LC/HP; P = 0.007 and P = 0.008, respectively) and week 8 (HC/LP vs MC/MP; P = 0.04), no differences between experimental groups were detected after 12 weeks of feeding. Finally, at the end of the trial, there was a negative correlation between lactic acid bacteria (LAB) ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Animal Nutrition 10 261 279 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Animal culture SF1-1100 |
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Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Animal culture SF1-1100 Alejandro Villasante Carolina Ramírez Héctor Rodríguez Patricio Dantagnan Adrián Hernández Elías Figueroa Jaime Romero Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
topic_facet |
Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics Animal culture SF1-1100 |
description |
Atlantic salmon (Salmo salar) fed a carbohydrate-rich diet exhibit suboptimal growth performance, along with other metabolic disturbances. It is well known that gut microbes play a pivotal role in influencing metabolism of the host, and these microbes can be modified by the diet. The main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times (i.e., weeks 4, 8 and 12), during a 12-week trial. A low carbohydrate-to-high protein diet (LC/HP, 0% wheat starch), a medium carbohydrate-to-medium protein diet (MC/MP, 15% wheat starch) or a high carbohydrate-to-low protein diet (HC/LP, 30% wheat starch) was fed to triplicate fish tanks (27 to 28 fish per tank). We performed an in-depth characterization of the distal intestine digesta microbiota. Further, growth parameters, liver histology and the expression of genes involved in hepatic neolipogenesis in fish were measured. Fish fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes (P = 0.026 and P = 0.018, respectively), lower final weight (P = 0.005), weight gain (P = 0.003), feed efficiency (P = 0.033) and growth rate (P = 0.003) compared with fish fed the LC/HP diet. Further, feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization, steatosis index (P = 0.007) and expression of fatty acid synthase (fas) and delta-6 fatty acyl desaturase (d6fad) (P = 0.001 and P = 0.001, respectively) in the liver compared with fish fed the LC/HP diet. Although, the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4 (HC/LP vs MC/MP and HC/LP vs LC/HP; P = 0.007 and P = 0.008, respectively) and week 8 (HC/LP vs MC/MP; P = 0.04), no differences between experimental groups were detected after 12 weeks of feeding. Finally, at the end of the trial, there was a negative correlation between lactic acid bacteria (LAB) ... |
format |
Article in Journal/Newspaper |
author |
Alejandro Villasante Carolina Ramírez Héctor Rodríguez Patricio Dantagnan Adrián Hernández Elías Figueroa Jaime Romero |
author_facet |
Alejandro Villasante Carolina Ramírez Héctor Rodríguez Patricio Dantagnan Adrián Hernández Elías Figueroa Jaime Romero |
author_sort |
Alejandro Villasante |
title |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_short |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_full |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_fullStr |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_full_unstemmed |
Dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (Salmo salar) |
title_sort |
dietary carbohydrate-to-protein ratio influences growth performance, hepatic health and dynamic of gut microbiota in atlantic salmon (salmo salar) |
publisher |
KeAi Communications Co., Ltd. |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.aninu.2022.04.003 https://doaj.org/article/2a2b9da6b9924a0ebfdae0928d8a48a2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Animal Nutrition, Vol 10, Iss , Pp 261-279 (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2405654522000427 https://doaj.org/toc/2405-6545 2405-6545 doi:10.1016/j.aninu.2022.04.003 https://doaj.org/article/2a2b9da6b9924a0ebfdae0928d8a48a2 |
op_doi |
https://doi.org/10.1016/j.aninu.2022.04.003 |
container_title |
Animal Nutrition |
container_volume |
10 |
container_start_page |
261 |
op_container_end_page |
279 |
_version_ |
1766360865080082432 |