In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia
Objective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea). Methods: Essential oil obtained by steam distillation were analyzed by ga...
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Wolters Kluwer Medknow Publications
2014
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ftdoajarticles:oai:doaj.org/article:2a0a396e7a69499cb718d53ff34a6258 2023-05-15T15:13:31+02:00 In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche 2014-07-01T00:00:00Z https://doi.org/10.12980/APJTB.4.201414B114 https://doaj.org/article/2a0a396e7a69499cb718d53ff34a6258 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115300654 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B114 https://doaj.org/article/2a0a396e7a69499cb718d53ff34a6258 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S649-S655 (2014) Chemical composition α-Amylase Lipase Essential oil Antioxidant activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.201414B114 2022-12-31T03:13:22Z Objective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea). Methods: Essential oil obtained by steam distillation were analyzed by gas chromatography-mass spectrometry technique. The antioxidant activity of the essential oil and various extracts of J. phoenicea were determined by DPPH and β-carotene bleaching methods. Results: Gas chromatography-mass spectrometry analysis of the J. phoenicea essential oil resulted in the identification of 37 compounds, representing 96.98% of the oil; α-Pinene (24.02%), limonene (7.94%), D-3-Carene (16.9%), Germacrene D (11.98%), Germacrene B (5.40%) and δ-cadinene (6.52%) were the major compounds. The IC50 values of essential oil, hexane and methanol extracts against α-amylase were 35.44, 30.15 and 53.76 μg/mL respectively, and those against pancreatic lipase were 66.15, 68.47 and 60.22 μg/mL respectively, suggesting powerful anti-diabetic and anti-obesity effects. Antioxidant activity (IC50=2 μg/mL) and total phenolics content (265 mg as gallic acid equivalent/g extract) of the methanol extract were found to be the highest compared to the other extracts. Conclusions: The findings showed that the extents of α-amylase and pancreatic lipase inhibitory activities of the J. phoenicea extracts as well as their antioxidant activity are in accordance with total phenolics contents. Leaves of J. phoenicea being rich in phenolics may provide a good source of natural products with interesting medicinal properties. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S649 S655 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chemical composition α-Amylase Lipase Essential oil Antioxidant activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Chemical composition α-Amylase Lipase Essential oil Antioxidant activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
topic_facet |
Chemical composition α-Amylase Lipase Essential oil Antioxidant activity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine chemical composition and antioxidant activity as well as the in vitro α-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea). Methods: Essential oil obtained by steam distillation were analyzed by gas chromatography-mass spectrometry technique. The antioxidant activity of the essential oil and various extracts of J. phoenicea were determined by DPPH and β-carotene bleaching methods. Results: Gas chromatography-mass spectrometry analysis of the J. phoenicea essential oil resulted in the identification of 37 compounds, representing 96.98% of the oil; α-Pinene (24.02%), limonene (7.94%), D-3-Carene (16.9%), Germacrene D (11.98%), Germacrene B (5.40%) and δ-cadinene (6.52%) were the major compounds. The IC50 values of essential oil, hexane and methanol extracts against α-amylase were 35.44, 30.15 and 53.76 μg/mL respectively, and those against pancreatic lipase were 66.15, 68.47 and 60.22 μg/mL respectively, suggesting powerful anti-diabetic and anti-obesity effects. Antioxidant activity (IC50=2 μg/mL) and total phenolics content (265 mg as gallic acid equivalent/g extract) of the methanol extract were found to be the highest compared to the other extracts. Conclusions: The findings showed that the extents of α-amylase and pancreatic lipase inhibitory activities of the J. phoenicea extracts as well as their antioxidant activity are in accordance with total phenolics contents. Leaves of J. phoenicea being rich in phenolics may provide a good source of natural products with interesting medicinal properties. |
format |
Article in Journal/Newspaper |
author |
Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche |
author_facet |
Henda Keskes Kais Mnafgui Khaled Hamden Mohamed Damak Abdelfattah El Feki Noureddine Allouche |
author_sort |
Henda Keskes |
title |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_short |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_full |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_fullStr |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_full_unstemmed |
In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from Tunisia |
title_sort |
in vitro anti-diabetic, anti-obesity and antioxidant proprieties of juniperus phoenicea l. leaves from tunisia |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.201414B114 https://doaj.org/article/2a0a396e7a69499cb718d53ff34a6258 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S2, Pp S649-S655 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115300654 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.201414B114 https://doaj.org/article/2a0a396e7a69499cb718d53ff34a6258 |
op_doi |
https://doi.org/10.12980/APJTB.4.201414B114 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S649 |
op_container_end_page |
S655 |
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1766344069726863360 |