Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon.
BACKGROUND:Culex species are widespread across Cameroon and responsible for high burden of nuisance in most urban settings. However, despite their high nuisance, they remain less studied compared to anophelines. The present study aimed to assess Culex species distribution, susceptibility to insectic...
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ftdoajarticles:oai:doaj.org/article:29e4e4131ac346218a06f6da5725047a 2023-05-15T15:14:30+02:00 Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. Elysee Nchoutpouen Abdou Talipouo Borel Djiappi-Tchamen Landre Djamouko-Djonkam Edmond Kopya Carmene Sandra Ngadjeu Patricia Doumbe-Belisse Parfait Awono-Ambene Sevilor Kekeunou Charles Sinclair Wondji Christophe Antonio-Nkondjio 2019-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007229 https://doaj.org/article/29e4e4131ac346218a06f6da5725047a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6464241?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007229 https://doaj.org/article/29e4e4131ac346218a06f6da5725047a PLoS Neglected Tropical Diseases, Vol 13, Iss 4, p e0007229 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007229 2022-12-31T11:55:26Z BACKGROUND:Culex species are widespread across Cameroon and responsible for high burden of nuisance in most urban settings. However, despite their high nuisance, they remain less studied compared to anophelines. The present study aimed to assess Culex species distribution, susceptibility to insecticide, bionomics and role in Lymphatic Filariasis (LF) transmission in the city of Yaoundé. METHODS:Mosquito collections were conducted from March to December 2017 using Centre for Disease Control light traps (CDC-LT), human landing catches (HLC) and larval collections. Mosquitoes were identified using morphological identification keys. Mosquitoes from the Culex pipiens complex were further identified using Polymerase Chain Reaction (PCR) to assess the presence of sibling species. Bioassays were conducted with 2-5 day-old unfed females to assess mosquito susceptibility to DDT, permethrin, deltamethrin and bendiocarb following WHO guidelines. Dead, control and surviving mosquitoes from bioassays were screened by PCR to detect the presence of knockdown resistance (kdr) alleles. Pools of mosquitoes were examined by PCR to detect the presence of Wuchereria bancrofti. RESULTS:A total of 197,956 mosquitoes belonging to thirteen species were collected. The density of mosquito collected varied according to the collection methods, districts and seasons. Culex quinquefasciatus emerged as the most abundant and the only species of the Culex pipiens complex in Yaoundé. Culex species were found breeding in different types of breeding sites including polluted and unpolluted sites. All Culex species including Cx antennatus, Cx duttoni, Cx perfuscus and Cx tigripes were found to be highly resistant to permethrin, deltamethrin and DDT. Culex quinquefasciatus was also found to be resistant to bendiocarb. A high frequency of the West Africa kdr allele was recorded in resistant Cx. quinquefasciatus. Out of the 247 pooled samples of 25 Culex spp. examined for the presence of Wuchereria bancrofti, none was found infected. CONCLUSION:The study ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 4 e0007229 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Elysee Nchoutpouen Abdou Talipouo Borel Djiappi-Tchamen Landre Djamouko-Djonkam Edmond Kopya Carmene Sandra Ngadjeu Patricia Doumbe-Belisse Parfait Awono-Ambene Sevilor Kekeunou Charles Sinclair Wondji Christophe Antonio-Nkondjio Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Culex species are widespread across Cameroon and responsible for high burden of nuisance in most urban settings. However, despite their high nuisance, they remain less studied compared to anophelines. The present study aimed to assess Culex species distribution, susceptibility to insecticide, bionomics and role in Lymphatic Filariasis (LF) transmission in the city of Yaoundé. METHODS:Mosquito collections were conducted from March to December 2017 using Centre for Disease Control light traps (CDC-LT), human landing catches (HLC) and larval collections. Mosquitoes were identified using morphological identification keys. Mosquitoes from the Culex pipiens complex were further identified using Polymerase Chain Reaction (PCR) to assess the presence of sibling species. Bioassays were conducted with 2-5 day-old unfed females to assess mosquito susceptibility to DDT, permethrin, deltamethrin and bendiocarb following WHO guidelines. Dead, control and surviving mosquitoes from bioassays were screened by PCR to detect the presence of knockdown resistance (kdr) alleles. Pools of mosquitoes were examined by PCR to detect the presence of Wuchereria bancrofti. RESULTS:A total of 197,956 mosquitoes belonging to thirteen species were collected. The density of mosquito collected varied according to the collection methods, districts and seasons. Culex quinquefasciatus emerged as the most abundant and the only species of the Culex pipiens complex in Yaoundé. Culex species were found breeding in different types of breeding sites including polluted and unpolluted sites. All Culex species including Cx antennatus, Cx duttoni, Cx perfuscus and Cx tigripes were found to be highly resistant to permethrin, deltamethrin and DDT. Culex quinquefasciatus was also found to be resistant to bendiocarb. A high frequency of the West Africa kdr allele was recorded in resistant Cx. quinquefasciatus. Out of the 247 pooled samples of 25 Culex spp. examined for the presence of Wuchereria bancrofti, none was found infected. CONCLUSION:The study ... |
format |
Article in Journal/Newspaper |
author |
Elysee Nchoutpouen Abdou Talipouo Borel Djiappi-Tchamen Landre Djamouko-Djonkam Edmond Kopya Carmene Sandra Ngadjeu Patricia Doumbe-Belisse Parfait Awono-Ambene Sevilor Kekeunou Charles Sinclair Wondji Christophe Antonio-Nkondjio |
author_facet |
Elysee Nchoutpouen Abdou Talipouo Borel Djiappi-Tchamen Landre Djamouko-Djonkam Edmond Kopya Carmene Sandra Ngadjeu Patricia Doumbe-Belisse Parfait Awono-Ambene Sevilor Kekeunou Charles Sinclair Wondji Christophe Antonio-Nkondjio |
author_sort |
Elysee Nchoutpouen |
title |
Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
title_short |
Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
title_full |
Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
title_fullStr |
Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
title_full_unstemmed |
Culex species diversity, susceptibility to insecticides and role as potential vector of Lymphatic filariasis in the city of Yaoundé, Cameroon. |
title_sort |
culex species diversity, susceptibility to insecticides and role as potential vector of lymphatic filariasis in the city of yaoundé, cameroon. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007229 https://doaj.org/article/29e4e4131ac346218a06f6da5725047a |
geographic |
Arctic |
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Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 4, p e0007229 (2019) |
op_relation |
http://europepmc.org/articles/PMC6464241?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007229 https://doaj.org/article/29e4e4131ac346218a06f6da5725047a |
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https://doi.org/10.1371/journal.pntd.0007229 |
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PLOS Neglected Tropical Diseases |
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