Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages.
BACKGROUND:Extracellular vesicles (EVs) are structures with phospholipid bilayer membranes and 100-1000 nm diameters. These vesicles are released from cells upon activation of surface receptors and/or apoptosis. The production of EVs by dendritic cells, mast cells, macrophages, and B and T lymphocyt...
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ftdoajarticles:oai:doaj.org/article:29cf3ae262694dec8ef420708c7289cd 2023-05-15T15:09:53+02:00 Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. André Cronemberger-Andrade Luciana Aragão-França Cintia Figueiredo de Araujo Viviane Junqueira Rocha Mariana da Cruz Borges-Silva Cláudio P Figueira Pablo R Oliveira Luiz A R de Freitas Patrícia S T Veras Lain Pontes-de-Carvalho 2014-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003161 https://doaj.org/article/29cf3ae262694dec8ef420708c7289cd EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4169249?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003161 https://doaj.org/article/29cf3ae262694dec8ef420708c7289cd PLoS Neglected Tropical Diseases, Vol 8, Iss 9, p e3161 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003161 2022-12-31T13:43:28Z BACKGROUND:Extracellular vesicles (EVs) are structures with phospholipid bilayer membranes and 100-1000 nm diameters. These vesicles are released from cells upon activation of surface receptors and/or apoptosis. The production of EVs by dendritic cells, mast cells, macrophages, and B and T lymphocytes has been extensively reported in the literature. EVs may express MHC class II and other membrane surface molecules and carry antigens. The aim of this study was to investigate the role of EVs from Leishmania-infected macrophages as immune modulatory particles. METHODOLOGY/PRINCIPAL FINDINGS:In this work it was shown that BALB/c mouse bone marrow-derived macrophages, either infected in vitro with Leishmania amazonensis or left uninfected, release comparable amounts of 50-300 nm-diameter extracellular vesicles (EVs). The EVs were characterized by flow cytometry and electron microscopy. The incubation of naïve macrophages with these EVs for 48 hours led to a statistically significant increase in the production of the cytokines IL-12, IL-1β, and TNF-α. CONCLUSIONS/SIGNIFICANCE:EVs derived from macrophages infected with L. amazonensis induce other macrophages, which in vivo could be bystander cells, to produce the proinflammatory cytokines IL-12, IL-1β and TNF-α. This could contribute both to modulate the immune system in favor of a Th1 immune response and to the elimination of the Leishmania, leading, therefore, to the control the infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 9 e3161 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 André Cronemberger-Andrade Luciana Aragão-França Cintia Figueiredo de Araujo Viviane Junqueira Rocha Mariana da Cruz Borges-Silva Cláudio P Figueira Pablo R Oliveira Luiz A R de Freitas Patrícia S T Veras Lain Pontes-de-Carvalho Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Extracellular vesicles (EVs) are structures with phospholipid bilayer membranes and 100-1000 nm diameters. These vesicles are released from cells upon activation of surface receptors and/or apoptosis. The production of EVs by dendritic cells, mast cells, macrophages, and B and T lymphocytes has been extensively reported in the literature. EVs may express MHC class II and other membrane surface molecules and carry antigens. The aim of this study was to investigate the role of EVs from Leishmania-infected macrophages as immune modulatory particles. METHODOLOGY/PRINCIPAL FINDINGS:In this work it was shown that BALB/c mouse bone marrow-derived macrophages, either infected in vitro with Leishmania amazonensis or left uninfected, release comparable amounts of 50-300 nm-diameter extracellular vesicles (EVs). The EVs were characterized by flow cytometry and electron microscopy. The incubation of naïve macrophages with these EVs for 48 hours led to a statistically significant increase in the production of the cytokines IL-12, IL-1β, and TNF-α. CONCLUSIONS/SIGNIFICANCE:EVs derived from macrophages infected with L. amazonensis induce other macrophages, which in vivo could be bystander cells, to produce the proinflammatory cytokines IL-12, IL-1β and TNF-α. This could contribute both to modulate the immune system in favor of a Th1 immune response and to the elimination of the Leishmania, leading, therefore, to the control the infection. |
format |
Article in Journal/Newspaper |
author |
André Cronemberger-Andrade Luciana Aragão-França Cintia Figueiredo de Araujo Viviane Junqueira Rocha Mariana da Cruz Borges-Silva Cláudio P Figueira Pablo R Oliveira Luiz A R de Freitas Patrícia S T Veras Lain Pontes-de-Carvalho |
author_facet |
André Cronemberger-Andrade Luciana Aragão-França Cintia Figueiredo de Araujo Viviane Junqueira Rocha Mariana da Cruz Borges-Silva Cláudio P Figueira Pablo R Oliveira Luiz A R de Freitas Patrícia S T Veras Lain Pontes-de-Carvalho |
author_sort |
André Cronemberger-Andrade |
title |
Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
title_short |
Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
title_full |
Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
title_fullStr |
Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
title_full_unstemmed |
Extracellular vesicles from Leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
title_sort |
extracellular vesicles from leishmania-infected macrophages confer an anti-infection cytokine-production profile to naïve macrophages. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003161 https://doaj.org/article/29cf3ae262694dec8ef420708c7289cd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 9, p e3161 (2014) |
op_relation |
http://europepmc.org/articles/PMC4169249?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003161 https://doaj.org/article/29cf3ae262694dec8ef420708c7289cd |
op_doi |
https://doi.org/10.1371/journal.pntd.0003161 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
9 |
container_start_page |
e3161 |
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1766340978962071552 |