Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues.
Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptos...
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ftdoajarticles:oai:doaj.org/article:29c3d263b13b42c7ad12c02b8b184708 2023-05-15T15:06:43+02:00 Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. J Muse Davis David A Haake Lalita Ramakrishnan 2009-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000463 https://doaj.org/article/29c3d263b13b42c7ad12c02b8b184708 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2693671?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000463 https://doaj.org/article/29c3d263b13b42c7ad12c02b8b184708 PLoS Neglected Tropical Diseases, Vol 3, Iss 6, p e463 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000463 2022-12-31T16:22:45Z Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptospires, we used fluorescently labelled Leptospira interrogans serovar Copenhageni to infect 30 hour post fertilization zebrafish embryos by either the caudal vein or hindbrain ventricle. These embryos have functional innate immunity but have not yet developed an adaptive immune system. Furthermore, they are optically transparent, allowing direct visualization of host-pathogen interactions from the moment of infection. We observed rapid uptake of leptospires by phagocytes, followed by persistent, intracellular infection over the first 48 hours. Phagocytosis of leptospires occasionally resulted in formation of large cellular vesicles consistent with apoptotic bodies. By 24 hours, clusters of infected phagocytes were accumulating lateral to the dorsal artery, presumably in early hematopoietic tissue. Our observations suggest that phagocytosis may be a key defense mechanism in the early stages of leptospirosis, and that phagocytic cells play roles in immunopathogenesis and likely in the dissemination of leptospires to specific target tissues. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 3 6 e463 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 J Muse Davis David A Haake Lalita Ramakrishnan Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptospires, we used fluorescently labelled Leptospira interrogans serovar Copenhageni to infect 30 hour post fertilization zebrafish embryos by either the caudal vein or hindbrain ventricle. These embryos have functional innate immunity but have not yet developed an adaptive immune system. Furthermore, they are optically transparent, allowing direct visualization of host-pathogen interactions from the moment of infection. We observed rapid uptake of leptospires by phagocytes, followed by persistent, intracellular infection over the first 48 hours. Phagocytosis of leptospires occasionally resulted in formation of large cellular vesicles consistent with apoptotic bodies. By 24 hours, clusters of infected phagocytes were accumulating lateral to the dorsal artery, presumably in early hematopoietic tissue. Our observations suggest that phagocytosis may be a key defense mechanism in the early stages of leptospirosis, and that phagocytic cells play roles in immunopathogenesis and likely in the dissemination of leptospires to specific target tissues. |
format |
Article in Journal/Newspaper |
author |
J Muse Davis David A Haake Lalita Ramakrishnan |
author_facet |
J Muse Davis David A Haake Lalita Ramakrishnan |
author_sort |
J Muse Davis |
title |
Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
title_short |
Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
title_full |
Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
title_fullStr |
Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
title_full_unstemmed |
Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
title_sort |
leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000463 https://doaj.org/article/29c3d263b13b42c7ad12c02b8b184708 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 6, p e463 (2009) |
op_relation |
http://europepmc.org/articles/PMC2693671?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000463 https://doaj.org/article/29c3d263b13b42c7ad12c02b8b184708 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000463 |
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PLoS Neglected Tropical Diseases |
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3 |
container_issue |
6 |
container_start_page |
e463 |
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1766338277513625600 |