Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660
Abstract Background While the genus Variovorax is known for its aromatic compound metabolism, no detailed study of the peripheral and central pathways of aromatic compound degradation has yet been reported. Variovorax sp. PAMC26660 is a lichen-associated bacterium isolated from Antarctica. The work...
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ftdoajarticles:oai:doaj.org/article:297dce5684b14590a211ea0744c153b5 2023-05-15T13:50:34+02:00 Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 Nisha Ghimire Byeollee Kim Chang-Muk Lee Tae-Jin Oh 2022-05-01T00:00:00Z https://doi.org/10.1186/s12864-022-08589-3 https://doaj.org/article/297dce5684b14590a211ea0744c153b5 EN eng BMC https://doi.org/10.1186/s12864-022-08589-3 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-022-08589-3 1471-2164 https://doaj.org/article/297dce5684b14590a211ea0744c153b5 BMC Genomics, Vol 23, Iss 1, Pp 1-16 (2022) Variovorax species Genome Pan-genome Aromatic compound degradation 4-hydroxybenzoate Biotechnology TP248.13-248.65 Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.1186/s12864-022-08589-3 2022-12-31T02:47:04Z Abstract Background While the genus Variovorax is known for its aromatic compound metabolism, no detailed study of the peripheral and central pathways of aromatic compound degradation has yet been reported. Variovorax sp. PAMC26660 is a lichen-associated bacterium isolated from Antarctica. The work presents the genome-based elucidation of peripheral and central catabolic pathways of aromatic compound degradation genes in Variovorax sp. PAMC26660. Additionally, the accessory, core and unique genes were identified among Variovorax species using the pan genome analysis tool. A detailed analysis of the genes related to xenobiotic metabolism revealed the potential roles of Variovorax sp. PAMC26660 and other species in bioremediation. Results TYGS analysis, dDDH, phylogenetic placement and average nucleotide identity (ANI) analysis identified the strain as Variovorax sp. Cell morphology was assessed using scanning electron microscopy (SEM). On analysis of the core, accessory, and unique genes, xenobiotic metabolism accounted only for the accessory and unique genes. On detailed analysis of the aromatic compound catabolic genes, peripheral pathway related to 4-hydroxybenzoate (4-HB) degradation was found among all species while phenylacetate and tyrosine degradation pathways were present in most of the species including PAMC26660. Likewise, central catabolic pathways, like protocatechuate, gentisate, homogentisate, and phenylacetyl-CoA, were also present. The peripheral pathway for 4-HB degradation was functionally tested using PAMC26660, which resulted in the growth using it as a sole source of carbon. Conclusions Computational tools for genome and pan genome analysis are important to understand the behavior of an organism. Xenobiotic metabolism-related genes, that only account for the accessory and unique genes infer evolution through events like lateral gene transfer, mutation and gene rearrangement. 4-HB, an aromatic compound present among lichen species is utilized by lichen-associated Variovorax sp. PAMC26660 as ... Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles BMC Genomics 23 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Variovorax species Genome Pan-genome Aromatic compound degradation 4-hydroxybenzoate Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Variovorax species Genome Pan-genome Aromatic compound degradation 4-hydroxybenzoate Biotechnology TP248.13-248.65 Genetics QH426-470 Nisha Ghimire Byeollee Kim Chang-Muk Lee Tae-Jin Oh Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
topic_facet |
Variovorax species Genome Pan-genome Aromatic compound degradation 4-hydroxybenzoate Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background While the genus Variovorax is known for its aromatic compound metabolism, no detailed study of the peripheral and central pathways of aromatic compound degradation has yet been reported. Variovorax sp. PAMC26660 is a lichen-associated bacterium isolated from Antarctica. The work presents the genome-based elucidation of peripheral and central catabolic pathways of aromatic compound degradation genes in Variovorax sp. PAMC26660. Additionally, the accessory, core and unique genes were identified among Variovorax species using the pan genome analysis tool. A detailed analysis of the genes related to xenobiotic metabolism revealed the potential roles of Variovorax sp. PAMC26660 and other species in bioremediation. Results TYGS analysis, dDDH, phylogenetic placement and average nucleotide identity (ANI) analysis identified the strain as Variovorax sp. Cell morphology was assessed using scanning electron microscopy (SEM). On analysis of the core, accessory, and unique genes, xenobiotic metabolism accounted only for the accessory and unique genes. On detailed analysis of the aromatic compound catabolic genes, peripheral pathway related to 4-hydroxybenzoate (4-HB) degradation was found among all species while phenylacetate and tyrosine degradation pathways were present in most of the species including PAMC26660. Likewise, central catabolic pathways, like protocatechuate, gentisate, homogentisate, and phenylacetyl-CoA, were also present. The peripheral pathway for 4-HB degradation was functionally tested using PAMC26660, which resulted in the growth using it as a sole source of carbon. Conclusions Computational tools for genome and pan genome analysis are important to understand the behavior of an organism. Xenobiotic metabolism-related genes, that only account for the accessory and unique genes infer evolution through events like lateral gene transfer, mutation and gene rearrangement. 4-HB, an aromatic compound present among lichen species is utilized by lichen-associated Variovorax sp. PAMC26660 as ... |
format |
Article in Journal/Newspaper |
author |
Nisha Ghimire Byeollee Kim Chang-Muk Lee Tae-Jin Oh |
author_facet |
Nisha Ghimire Byeollee Kim Chang-Muk Lee Tae-Jin Oh |
author_sort |
Nisha Ghimire |
title |
Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
title_short |
Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
title_full |
Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
title_fullStr |
Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
title_full_unstemmed |
Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 |
title_sort |
comparative genome analysis among variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in variovorax sp. pamc26660 |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12864-022-08589-3 https://doaj.org/article/297dce5684b14590a211ea0744c153b5 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
BMC Genomics, Vol 23, Iss 1, Pp 1-16 (2022) |
op_relation |
https://doi.org/10.1186/s12864-022-08589-3 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-022-08589-3 1471-2164 https://doaj.org/article/297dce5684b14590a211ea0744c153b5 |
op_doi |
https://doi.org/10.1186/s12864-022-08589-3 |
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BMC Genomics |
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23 |
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1 |
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1766253734744031232 |