A coproantigen diagnostic test for Strongyloides infection.
Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfact...
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ftdoajarticles:oai:doaj.org/article:2975ea18906f4e4f8eec508f533d3365 2023-05-15T15:05:40+02:00 A coproantigen diagnostic test for Strongyloides infection. Alex M Sykes James S McCarthy 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000955 https://doaj.org/article/2975ea18906f4e4f8eec508f533d3365 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3035667?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000955 https://doaj.org/article/2975ea18906f4e4f8eec508f533d3365 PLoS Neglected Tropical Diseases, Vol 5, Iss 2, p e955 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000955 2022-12-31T01:37:10Z Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. In this manuscript we describe the development of a coproantigen ELISA for diagnosis of infection. Polyclonal rabbit antiserum was raised against Strongyloides ratti excretory/secretory (E/S) antigen and utilized to develop an antigen capture ELISA. The assay enabled detection of subpatent rodent S. ratti and human S. stercoralis infection. No cross-reactivity was observed with purified E/S from Schistosoma japonicum, the hookworms Ancylostoma caninum, A. ceylanicum, nor with fecal samples collected from rodents harboring Trichuris muris or S. mansoni infection. Strongyloides coproantigens that appear stable when frozen as formalin-extracted fecal supernatants stored at -20 °C remained positive up to 270 days of storage, whereas supernatants stored at 4 °C tested negative. These results indicate that diagnosis of human strongyloidiasis by detection of coproantigen is an approach worthy of further development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 2 e955 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alex M Sykes James S McCarthy A coproantigen diagnostic test for Strongyloides infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. In this manuscript we describe the development of a coproantigen ELISA for diagnosis of infection. Polyclonal rabbit antiserum was raised against Strongyloides ratti excretory/secretory (E/S) antigen and utilized to develop an antigen capture ELISA. The assay enabled detection of subpatent rodent S. ratti and human S. stercoralis infection. No cross-reactivity was observed with purified E/S from Schistosoma japonicum, the hookworms Ancylostoma caninum, A. ceylanicum, nor with fecal samples collected from rodents harboring Trichuris muris or S. mansoni infection. Strongyloides coproantigens that appear stable when frozen as formalin-extracted fecal supernatants stored at -20 °C remained positive up to 270 days of storage, whereas supernatants stored at 4 °C tested negative. These results indicate that diagnosis of human strongyloidiasis by detection of coproantigen is an approach worthy of further development. |
format |
Article in Journal/Newspaper |
author |
Alex M Sykes James S McCarthy |
author_facet |
Alex M Sykes James S McCarthy |
author_sort |
Alex M Sykes |
title |
A coproantigen diagnostic test for Strongyloides infection. |
title_short |
A coproantigen diagnostic test for Strongyloides infection. |
title_full |
A coproantigen diagnostic test for Strongyloides infection. |
title_fullStr |
A coproantigen diagnostic test for Strongyloides infection. |
title_full_unstemmed |
A coproantigen diagnostic test for Strongyloides infection. |
title_sort |
coproantigen diagnostic test for strongyloides infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0000955 https://doaj.org/article/2975ea18906f4e4f8eec508f533d3365 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 2, p e955 (2011) |
op_relation |
http://europepmc.org/articles/PMC3035667?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000955 https://doaj.org/article/2975ea18906f4e4f8eec508f533d3365 |
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https://doi.org/10.1371/journal.pntd.0000955 |
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PLoS Neglected Tropical Diseases |
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5 |
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2 |
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e955 |
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