Prolonged exposure to elevated CO2 promotes growth of the algal symbiont Symbiodinium muscatinei in the intertidal sea anemone Anthopleura elegantissima

• Published in: Biology Open
• Main Authors: Trisha Towanda, Erik V. Thuesen
• Format: Article in Journal/Newspaper
• Language: English
• Published: The Company of Biologists 2012
• Subjects: Carbon dioxide; Ocean acidification; Anthopleura elegantissima; Zooxanthellae; Metabolic rate; Photosynthesis; CZAR; Intertidal; Science; Q; Biology (General); QH301-705.5
• Online Access: https://doi.org/10.1242/bio.2012521; https://doaj.org/article/29400a2ced8142f396d8b575f8c548eb

Summary Some photosynthetic organisms benefit from elevated levels of carbon dioxide, but studies on the effects of elevated PCO2 on the algal symbionts of animals are very few. This study investigated the impact of hypercapnia on a photosynthetic symbiosis between the anemone Anthopleura elegantissima and its zooxanthella Symbiodinium muscatinei. Anemones were maintained in the laboratory for 1 week at 37 Pa PCO2 and pH 8.1. Clonal pairs were then divided into two groups and maintained for 6 weeks under conditions naturally experienced in their intertidal environment, 45 Pa PCO2, pH 8.1 and 231 Pa PCO2, pH 7.3. Respiration and photosynthesis were measured after the 1-week acclimation period and after 6 weeks in experimental conditions. Density of zooxanthellal cells, zooxanthellal cell size, mitotic index and chlorophyll content were compared between non-clonemate anemones after the 1-week acclimation period and clonal anemones at the end of the experiment. Anemones thrived in hypercapnia. After 6 weeks, A. elegantissima exhibited higher rates of photosynthesis at 45 Pa (4.2 µmol O2 g−1 h−1) and 231 Pa (3.30 µmol O2 g−1 h−1) than at the initial 37 Pa (1.53 µmol O2 g−1 h−1). Likewise, anemones at 231 Pa received more of their respiratory carbon from zooxanthellae (CZAR = 78.2%) than those at 37 Pa (CZAR = 66.6%) but less than anemones at 45 Pa (CZAR = 137.3%). The mitotic index of zooxanthellae was significantly greater in the hypercapnic anemones than in anemones at lower PCO2. Excess zooxanthellae were expelled by their hosts, and cell densities, cell diameters and chlorophyll contents were not significantly different between the groups. The response of A. elegantissima to hypercapnic acidification reveals the potential adaptation of an intertidal, photosynthetic symbiosis for high PCO2.