Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex.
Paracoccidioides brasiliensis is a dimorphic fungus that is the causative agent of paracoccidioidomycosis, the most important prevalent systemic mycosis in Latin America. Recently, the existence of three genetically isolated groups in P. brasiliensis was demonstrated, enabling comparative studies of...
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ftdoajarticles:oai:doaj.org/article:2901aadb522d4f2883f1b2865d67b752 2023-05-15T15:06:58+02:00 Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. Daniel R Matute Lina M Quesada-Ocampo Jason T Rauscher Juan G McEwen 2008-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000296 https://doaj.org/article/2901aadb522d4f2883f1b2865d67b752 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2553485?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000296 https://doaj.org/article/2901aadb522d4f2883f1b2865d67b752 PLoS Neglected Tropical Diseases, Vol 2, Iss 9, p e296 (2008) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000296 2022-12-30T22:10:48Z Paracoccidioides brasiliensis is a dimorphic fungus that is the causative agent of paracoccidioidomycosis, the most important prevalent systemic mycosis in Latin America. Recently, the existence of three genetically isolated groups in P. brasiliensis was demonstrated, enabling comparative studies of molecular evolution among P. brasiliensis lineages. Thirty-two gene sequences coding for putative virulence factors were analyzed to determine whether they were under positive selection. Our maximum likelihood-based approach yielded evidence for selection in 12 genes that are involved in different cellular processes. An in-depth analysis of four of these genes showed them to be either antigenic or involved in pathogenesis. Here, we present evidence indicating that several replacement mutations in gp43 are under positive balancing selection. The other three genes (fks, cdc42 and p27) show very little variation among the P. brasiliensis lineages and appear to be under positive directional selection. Our results are consistent with the more general observations that selective constraints are variable across the genome, and that even in the genes under positive selection, only a few sites are altered. We present our results within an evolutionary framework that may be applicable for studying adaptation and pathogenesis in P. brasiliensis and other pathogenic fungi. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 2 9 e296 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daniel R Matute Lina M Quesada-Ocampo Jason T Rauscher Juan G McEwen Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Paracoccidioides brasiliensis is a dimorphic fungus that is the causative agent of paracoccidioidomycosis, the most important prevalent systemic mycosis in Latin America. Recently, the existence of three genetically isolated groups in P. brasiliensis was demonstrated, enabling comparative studies of molecular evolution among P. brasiliensis lineages. Thirty-two gene sequences coding for putative virulence factors were analyzed to determine whether they were under positive selection. Our maximum likelihood-based approach yielded evidence for selection in 12 genes that are involved in different cellular processes. An in-depth analysis of four of these genes showed them to be either antigenic or involved in pathogenesis. Here, we present evidence indicating that several replacement mutations in gp43 are under positive balancing selection. The other three genes (fks, cdc42 and p27) show very little variation among the P. brasiliensis lineages and appear to be under positive directional selection. Our results are consistent with the more general observations that selective constraints are variable across the genome, and that even in the genes under positive selection, only a few sites are altered. We present our results within an evolutionary framework that may be applicable for studying adaptation and pathogenesis in P. brasiliensis and other pathogenic fungi. |
format |
Article in Journal/Newspaper |
author |
Daniel R Matute Lina M Quesada-Ocampo Jason T Rauscher Juan G McEwen |
author_facet |
Daniel R Matute Lina M Quesada-Ocampo Jason T Rauscher Juan G McEwen |
author_sort |
Daniel R Matute |
title |
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
title_short |
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
title_full |
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
title_fullStr |
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
title_full_unstemmed |
Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. |
title_sort |
evidence for positive selection in putative virulence factors within the paracoccidioides brasiliensis species complex. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doi.org/10.1371/journal.pntd.0000296 https://doaj.org/article/2901aadb522d4f2883f1b2865d67b752 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 2, Iss 9, p e296 (2008) |
op_relation |
http://europepmc.org/articles/PMC2553485?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000296 https://doaj.org/article/2901aadb522d4f2883f1b2865d67b752 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000296 |
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PLoS Neglected Tropical Diseases |
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2 |
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9 |
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e296 |
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