Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis
Abstract Background Anopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is know...
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ftdoajarticles:oai:doaj.org/article:28bf78c0d26a4feda519ad7f8767e180 2023-05-15T15:09:05+02:00 Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis Laurent-Winter C Prévot GI Rodhain F Bourgouin C 2003-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-2-1 https://doaj.org/article/28bf78c0d26a4feda519ad7f8767e180 EN eng BMC http://www.malariajournal.com/content/2/1/1 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-1 1475-2875 https://doaj.org/article/28bf78c0d26a4feda519ad7f8767e180 Malaria Journal, Vol 2, Iss 1, p 1 (2003) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1186/1475-2875-2-1 2022-12-30T21:45:31Z Abstract Background Anopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is known about its protein composition. Characterisation of An. gambiae midgut proteins may identify the proteins that render An. gambiae receptive to the malaria parasite. Methods We carried out two-dimensional gel electrophoresis of An. gambiae midgut proteins and compared protein profiles for midguts from males, sugar-fed females and females fed on human blood. Results Very few differences were detected between male and female mosquitoes for the approximately 375 silver-stained proteins. Male midguts contained ten proteins not detected in sugar-fed or blood-fed females, which are therefore probably involved in male-specific functions; conversely, female midguts contained twenty-three proteins absent from male midguts. Eight of these proteins were specific to sugar-fed females, and another ten, to blood-fed females. Conclusion Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An. gambiae genome, should make it possible to determine the role of these proteins in blood digestion or parasite receptivity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 2 1 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Laurent-Winter C Prévot GI Rodhain F Bourgouin C Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles gambiae is the main vector of Plasmodium falciparum in Africa. The mosquito midgut constitutes a barrier that the parasite must cross if it is to develop and be transmitted. Despite the central role of the mosquito midgut in the host/parasite interaction, little is known about its protein composition. Characterisation of An. gambiae midgut proteins may identify the proteins that render An. gambiae receptive to the malaria parasite. Methods We carried out two-dimensional gel electrophoresis of An. gambiae midgut proteins and compared protein profiles for midguts from males, sugar-fed females and females fed on human blood. Results Very few differences were detected between male and female mosquitoes for the approximately 375 silver-stained proteins. Male midguts contained ten proteins not detected in sugar-fed or blood-fed females, which are therefore probably involved in male-specific functions; conversely, female midguts contained twenty-three proteins absent from male midguts. Eight of these proteins were specific to sugar-fed females, and another ten, to blood-fed females. Conclusion Mass spectrometry analysis of the proteins found only in blood-fed female midguts, together with data from the recent sequencing of the An. gambiae genome, should make it possible to determine the role of these proteins in blood digestion or parasite receptivity. |
format |
Article in Journal/Newspaper |
author |
Laurent-Winter C Prévot GI Rodhain F Bourgouin C |
author_facet |
Laurent-Winter C Prévot GI Rodhain F Bourgouin C |
author_sort |
Laurent-Winter C |
title |
Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
title_short |
Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
title_full |
Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
title_fullStr |
Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
title_full_unstemmed |
Sex-specific and blood meal-induced proteins of Anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
title_sort |
sex-specific and blood meal-induced proteins of anopheles gambiae midguts: analysis by two-dimensional gel electrophoresis |
publisher |
BMC |
publishDate |
2003 |
url |
https://doi.org/10.1186/1475-2875-2-1 https://doaj.org/article/28bf78c0d26a4feda519ad7f8767e180 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 2, Iss 1, p 1 (2003) |
op_relation |
http://www.malariajournal.com/content/2/1/1 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-1 1475-2875 https://doaj.org/article/28bf78c0d26a4feda519ad7f8767e180 |
op_doi |
https://doi.org/10.1186/1475-2875-2-1 |
container_title |
Malaria Journal |
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2 |
container_issue |
1 |
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1 |
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1766340326718439424 |