Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs.
Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depend...
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ftdoajarticles:oai:doaj.org/article:2877ce95e1eb46e5a3b3cfb582b29ea9 2024-01-14T10:05:07+01:00 Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. Kadidiata Ilboudo Alain Boulangé Robert Eustache Hounyèmè Geoffrey Gimonneau Jacques Kaboré Adrien Gaston Marie Belem Marc Desquesnes Veerle Lejon Mathurin Koffi Vincent Jamonneau Sophie Thévenon 2023-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011730 https://doaj.org/article/2877ce95e1eb46e5a3b3cfb582b29ea9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011730 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011730 https://doaj.org/article/2877ce95e1eb46e5a3b3cfb582b29ea9 PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011730 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011730 2023-12-17T01:44:39Z Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depends on the performance of diagnostic tools, which is not well known. In pigs experimentally infected with Trypanosoma brucei brucei, we evaluated the performance of parasitological Buffy Coat Technique (BCT), two molecular (TBR and 5.8S PCR) and four serological tests (CATT, HAT Sero-K-Set rapid diagnostic test-RDT, indirect ELISA, immune trypanolysis). Most diagnostic tests showed high specificity, estimated at 100% (95% CI = 74-100%) with the exception of CATT and RDT whose specificity varied between 100% (95% CI = 74-100%) to 50% (95% CI = 7-93%) during the experiment. The sensitivity of each test fluctuated over the course of the infection. The percentage of positive BCT over the infection (30%) was lower than of positive PCR (56% and 62%, depending on primers). Among the serological tests, the percentage of positive tests was 97%, 96%, 86% and 84% for RDT, ELISA, immune trypanolysis and CATT, respectively. Fair agreement was observed between both molecular tests (κ = 0.36). Among the serological tests, the agreement between the ELISA and the RDT was substantial (κ = 0.65). Our results on the T.b. brucei infection model suggest that serological techniques are efficient in detecting the chronic phase of infection, PCR is able to detect positive samples several months after parasites inoculation while BCT becomes negative. BCT examination and RDT are useful to get a quick information in the field, and BCT can be used for treatment decision. ELISA appears most suited for epidemiological studies. The selection of diagnostic tests for trypanosomosis in pigs depends on the context, the objectives and the available resources. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 11 e0011730 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kadidiata Ilboudo Alain Boulangé Robert Eustache Hounyèmè Geoffrey Gimonneau Jacques Kaboré Adrien Gaston Marie Belem Marc Desquesnes Veerle Lejon Mathurin Koffi Vincent Jamonneau Sophie Thévenon Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depends on the performance of diagnostic tools, which is not well known. In pigs experimentally infected with Trypanosoma brucei brucei, we evaluated the performance of parasitological Buffy Coat Technique (BCT), two molecular (TBR and 5.8S PCR) and four serological tests (CATT, HAT Sero-K-Set rapid diagnostic test-RDT, indirect ELISA, immune trypanolysis). Most diagnostic tests showed high specificity, estimated at 100% (95% CI = 74-100%) with the exception of CATT and RDT whose specificity varied between 100% (95% CI = 74-100%) to 50% (95% CI = 7-93%) during the experiment. The sensitivity of each test fluctuated over the course of the infection. The percentage of positive BCT over the infection (30%) was lower than of positive PCR (56% and 62%, depending on primers). Among the serological tests, the percentage of positive tests was 97%, 96%, 86% and 84% for RDT, ELISA, immune trypanolysis and CATT, respectively. Fair agreement was observed between both molecular tests (κ = 0.36). Among the serological tests, the agreement between the ELISA and the RDT was substantial (κ = 0.65). Our results on the T.b. brucei infection model suggest that serological techniques are efficient in detecting the chronic phase of infection, PCR is able to detect positive samples several months after parasites inoculation while BCT becomes negative. BCT examination and RDT are useful to get a quick information in the field, and BCT can be used for treatment decision. ELISA appears most suited for epidemiological studies. The selection of diagnostic tests for trypanosomosis in pigs depends on the context, the objectives and the available resources. |
format |
Article in Journal/Newspaper |
author |
Kadidiata Ilboudo Alain Boulangé Robert Eustache Hounyèmè Geoffrey Gimonneau Jacques Kaboré Adrien Gaston Marie Belem Marc Desquesnes Veerle Lejon Mathurin Koffi Vincent Jamonneau Sophie Thévenon |
author_facet |
Kadidiata Ilboudo Alain Boulangé Robert Eustache Hounyèmè Geoffrey Gimonneau Jacques Kaboré Adrien Gaston Marie Belem Marc Desquesnes Veerle Lejon Mathurin Koffi Vincent Jamonneau Sophie Thévenon |
author_sort |
Kadidiata Ilboudo |
title |
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
title_short |
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
title_full |
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
title_fullStr |
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
title_full_unstemmed |
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. |
title_sort |
performance of diagnostic tests for trypanosoma brucei brucei in experimentally infected pigs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011730 https://doaj.org/article/2877ce95e1eb46e5a3b3cfb582b29ea9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0011730 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011730 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011730 https://doaj.org/article/2877ce95e1eb46e5a3b3cfb582b29ea9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011730 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
11 |
container_start_page |
e0011730 |
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1788059516154150912 |