G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon
Abstract Background Plasmodium vivax parasites are the predominant cause of malaria infections in the Brazilian Amazon. Infected individuals are treated with primaquine, which can induce haemolytic anaemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals and may lead to severe and f...
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ftdoajarticles:oai:doaj.org/article:2869eb46dd6d4109b31a40c39a524f3b 2023-05-15T15:16:42+02:00 G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon Jamille G. Dombrowski Rodrigo M. Souza Jonathan Curry Laura Hinton Natercia R. M. Silva Lynn Grignard Ligia A. Gonçalves Ana Rita Gomes Sabrina Epiphanio Chris Drakeley Jim Huggett Taane G. Clark Susana Campino Claudio R. F. Marinho 2017-06-01T00:00:00Z https://doi.org/10.1186/s12936-017-1889-6 https://doaj.org/article/2869eb46dd6d4109b31a40c39a524f3b EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1889-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1889-6 1475-2875 https://doaj.org/article/2869eb46dd6d4109b31a40c39a524f3b Malaria Journal, Vol 16, Iss 1, Pp 1-9 (2017) Malaria Glucose-6-phosphate dehydrogenase Genetic variants Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1889-6 2022-12-31T04:32:35Z Abstract Background Plasmodium vivax parasites are the predominant cause of malaria infections in the Brazilian Amazon. Infected individuals are treated with primaquine, which can induce haemolytic anaemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals and may lead to severe and fatal complications. This X-linked disorder is distributed globally and is caused by allelic variants with a geographical distribution that closely reflects populations exposed historically to endemic malaria. In Brazil, few studies have reported the frequency of G6PD deficiency (G6PDd) present in malaria-endemic areas. This is particularly important, as G6PDd screening is not currently performed before primaquine treatment. The aim of this study was to determine the prevalence of G6PDd in the region of Alto do Juruá, in the Western Brazilian Amazon, an area characterized by a high prevalence of P. vivax infection. Methods Five-hundred and sixteen male volunteers were screened for G6PDd using the fluorescence spot test (Beutler test) and CareStart™ G6PD Biosensor system. Demographic and clinical-epidemiological data were acquired through an individual interview. To assess the genetic basis of G6PDd, 24 SNPs were genotyped using the Kompetitive Allele Specific PCR assay. Results Twenty-three (4.5%) individuals were G6PDd. No association was found between G6PDd and the number of malaria cases. An increased risk of reported haemolysis symptoms and blood transfusions was evident among the G6PDd individuals. Twenty-two individuals had the G6PDd A(−) variant and one the G6PD A(+) variant. The Mediterranean variant was not present. Apart from one polymorphism, almost all SNPs were monomorphic or with low frequencies (0–0.04%). No differences were detected among ethnic groups. Conclusions The data indicates that ~1/23 males from the Alto do Juruá could be G6PD deficient and at risk of haemolytic anaemia if treated with primaquine. G6PD A(−) is the most frequent deficiency allele in this population. These results concur with ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Malaria Glucose-6-phosphate dehydrogenase Genetic variants Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Glucose-6-phosphate dehydrogenase Genetic variants Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jamille G. Dombrowski Rodrigo M. Souza Jonathan Curry Laura Hinton Natercia R. M. Silva Lynn Grignard Ligia A. Gonçalves Ana Rita Gomes Sabrina Epiphanio Chris Drakeley Jim Huggett Taane G. Clark Susana Campino Claudio R. F. Marinho G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
topic_facet |
Malaria Glucose-6-phosphate dehydrogenase Genetic variants Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium vivax parasites are the predominant cause of malaria infections in the Brazilian Amazon. Infected individuals are treated with primaquine, which can induce haemolytic anaemia in glucose-6-phosphate dehydrogenase (G6PD)-deficient individuals and may lead to severe and fatal complications. This X-linked disorder is distributed globally and is caused by allelic variants with a geographical distribution that closely reflects populations exposed historically to endemic malaria. In Brazil, few studies have reported the frequency of G6PD deficiency (G6PDd) present in malaria-endemic areas. This is particularly important, as G6PDd screening is not currently performed before primaquine treatment. The aim of this study was to determine the prevalence of G6PDd in the region of Alto do Juruá, in the Western Brazilian Amazon, an area characterized by a high prevalence of P. vivax infection. Methods Five-hundred and sixteen male volunteers were screened for G6PDd using the fluorescence spot test (Beutler test) and CareStart™ G6PD Biosensor system. Demographic and clinical-epidemiological data were acquired through an individual interview. To assess the genetic basis of G6PDd, 24 SNPs were genotyped using the Kompetitive Allele Specific PCR assay. Results Twenty-three (4.5%) individuals were G6PDd. No association was found between G6PDd and the number of malaria cases. An increased risk of reported haemolysis symptoms and blood transfusions was evident among the G6PDd individuals. Twenty-two individuals had the G6PDd A(−) variant and one the G6PD A(+) variant. The Mediterranean variant was not present. Apart from one polymorphism, almost all SNPs were monomorphic or with low frequencies (0–0.04%). No differences were detected among ethnic groups. Conclusions The data indicates that ~1/23 males from the Alto do Juruá could be G6PD deficient and at risk of haemolytic anaemia if treated with primaquine. G6PD A(−) is the most frequent deficiency allele in this population. These results concur with ... |
format |
Article in Journal/Newspaper |
author |
Jamille G. Dombrowski Rodrigo M. Souza Jonathan Curry Laura Hinton Natercia R. M. Silva Lynn Grignard Ligia A. Gonçalves Ana Rita Gomes Sabrina Epiphanio Chris Drakeley Jim Huggett Taane G. Clark Susana Campino Claudio R. F. Marinho |
author_facet |
Jamille G. Dombrowski Rodrigo M. Souza Jonathan Curry Laura Hinton Natercia R. M. Silva Lynn Grignard Ligia A. Gonçalves Ana Rita Gomes Sabrina Epiphanio Chris Drakeley Jim Huggett Taane G. Clark Susana Campino Claudio R. F. Marinho |
author_sort |
Jamille G. Dombrowski |
title |
G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
title_short |
G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
title_full |
G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
title_fullStr |
G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
title_full_unstemmed |
G6PD deficiency alleles in a malaria-endemic region in the Western Brazilian Amazon |
title_sort |
g6pd deficiency alleles in a malaria-endemic region in the western brazilian amazon |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1889-6 https://doaj.org/article/2869eb46dd6d4109b31a40c39a524f3b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-9 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1889-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1889-6 1475-2875 https://doaj.org/article/2869eb46dd6d4109b31a40c39a524f3b |
op_doi |
https://doi.org/10.1186/s12936-017-1889-6 |
container_title |
Malaria Journal |
container_volume |
16 |
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1 |
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1766346997322743808 |