Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis.
The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudoma...
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ftdoajarticles:oai:doaj.org/article:285a7bb2513b4029ac5b08bce2c36c4e 2023-05-15T15:13:46+02:00 Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. Erin P Price Derek S Sarovich Jessica R Webb Carina M Hall Sierra A Jaramillo Jason W Sahl Mirjam Kaestli Mark Mayo Glenda Harrington Anthony L Baker Lindsay C Sidak-Loftis Erik W Settles Madeline Lummis James M Schupp John D Gillece Apichai Tuanyok Jeffrey Warner Joseph D Busch Paul Keim Bart J Currie David M Wagner 2017-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005928 https://doaj.org/article/285a7bb2513b4029ac5b08bce2c36c4e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5614643?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005928 https://doaj.org/article/285a7bb2513b4029ac5b08bce2c36c4e PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005928 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005928 2022-12-31T01:14:03Z The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudomallei. Unlike most other Burkholderia cepacia complex (Bcc) species, B. ubonensis is considered non-pathogenic, although its virulence potential has not been tested. Antibiotic resistance in B. ubonensis, particularly towards drugs used to treat the most severe B. pseudomallei infections, has also been poorly characterised. This study examined the population biology of B. ubonensis, and includes the first reported isolates from the Caribbean. Phylogenomic analysis of 264 B. ubonensis genomes identified distinct clades that corresponded with geographic origin, similar to B. pseudomallei. A small proportion (4%) of strains lacked the 920kb chromosome III replicon, with discordance of presence/absence amongst genetically highly related strains, demonstrating that the third chromosome of B. ubonensis, like other Bcc species, probably encodes for a nonessential pC3 megaplasmid. Multilocus sequence typing using the B. pseudomallei scheme revealed that one-third of strains lack the "housekeeping" narK locus. In comparison, all strains could be genotyped using the Bcc scheme. Several strains possessed high-level meropenem resistance (≥32 μg/mL), a concern due to potential transmission of this phenotype to B. pseudomallei. In silico analysis uncovered a high degree of heterogeneity among the lipopolysaccharide O-antigen cluster loci, with at least 35 different variants identified. Finally, we show that Asian B. ubonensis isolate RF23-BP41 is avirulent in the BALB/c mouse model via a subcutaneous route of infection. Our results provide several new insights into the biology of this understudied species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 9 e0005928 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Erin P Price Derek S Sarovich Jessica R Webb Carina M Hall Sierra A Jaramillo Jason W Sahl Mirjam Kaestli Mark Mayo Glenda Harrington Anthony L Baker Lindsay C Sidak-Loftis Erik W Settles Madeline Lummis James M Schupp John D Gillece Apichai Tuanyok Jeffrey Warner Joseph D Busch Paul Keim Bart J Currie David M Wagner Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudomallei. Unlike most other Burkholderia cepacia complex (Bcc) species, B. ubonensis is considered non-pathogenic, although its virulence potential has not been tested. Antibiotic resistance in B. ubonensis, particularly towards drugs used to treat the most severe B. pseudomallei infections, has also been poorly characterised. This study examined the population biology of B. ubonensis, and includes the first reported isolates from the Caribbean. Phylogenomic analysis of 264 B. ubonensis genomes identified distinct clades that corresponded with geographic origin, similar to B. pseudomallei. A small proportion (4%) of strains lacked the 920kb chromosome III replicon, with discordance of presence/absence amongst genetically highly related strains, demonstrating that the third chromosome of B. ubonensis, like other Bcc species, probably encodes for a nonessential pC3 megaplasmid. Multilocus sequence typing using the B. pseudomallei scheme revealed that one-third of strains lack the "housekeeping" narK locus. In comparison, all strains could be genotyped using the Bcc scheme. Several strains possessed high-level meropenem resistance (≥32 μg/mL), a concern due to potential transmission of this phenotype to B. pseudomallei. In silico analysis uncovered a high degree of heterogeneity among the lipopolysaccharide O-antigen cluster loci, with at least 35 different variants identified. Finally, we show that Asian B. ubonensis isolate RF23-BP41 is avirulent in the BALB/c mouse model via a subcutaneous route of infection. Our results provide several new insights into the biology of this understudied species. |
format |
Article in Journal/Newspaper |
author |
Erin P Price Derek S Sarovich Jessica R Webb Carina M Hall Sierra A Jaramillo Jason W Sahl Mirjam Kaestli Mark Mayo Glenda Harrington Anthony L Baker Lindsay C Sidak-Loftis Erik W Settles Madeline Lummis James M Schupp John D Gillece Apichai Tuanyok Jeffrey Warner Joseph D Busch Paul Keim Bart J Currie David M Wagner |
author_facet |
Erin P Price Derek S Sarovich Jessica R Webb Carina M Hall Sierra A Jaramillo Jason W Sahl Mirjam Kaestli Mark Mayo Glenda Harrington Anthony L Baker Lindsay C Sidak-Loftis Erik W Settles Madeline Lummis James M Schupp John D Gillece Apichai Tuanyok Jeffrey Warner Joseph D Busch Paul Keim Bart J Currie David M Wagner |
author_sort |
Erin P Price |
title |
Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
title_short |
Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
title_full |
Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
title_fullStr |
Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
title_full_unstemmed |
Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis. |
title_sort |
phylogeographic, genomic, and meropenem susceptibility analysis of burkholderia ubonensis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005928 https://doaj.org/article/285a7bb2513b4029ac5b08bce2c36c4e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 9, p e0005928 (2017) |
op_relation |
http://europepmc.org/articles/PMC5614643?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005928 https://doaj.org/article/285a7bb2513b4029ac5b08bce2c36c4e |
op_doi |
https://doi.org/10.1371/journal.pntd.0005928 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
9 |
container_start_page |
e0005928 |
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