Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016)
ABSTRACT Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins...
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ftdoajarticles:oai:doaj.org/article:2844989403704b73ba63e5218b174251 2024-09-09T19:27:59+00:00 Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) Marcelle Silva-Sales Elcio Leal Flavio Augusto de Pádua Milagres Rafael Brustulin Vanessa dos Santos Morais Roberta Marcatti Emerson Luiz Lima Araújo Steven S. Witkin Xutao Deng Ester Cerdeira Sabino Eric Delwart Adriana Luchs Antonio Charlys da Costa 2020-12-01T00:00:00Z https://doi.org/10.1590/s1678-9946202062098 https://doaj.org/article/2844989403704b73ba63e5218b174251 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100248&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062098 https://doaj.org/article/2844989403704b73ba63e5218b174251 Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) Acute gastroenteritis Rotavirus A Brazil Post-vaccine Genomic Constellation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1590/s1678-9946202062098 2024-08-05T17:49:30Z ABSTRACT Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination follow-up (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%), as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G2P[4] (5.6%) and G8P[4] (2.8%) strains, including the mixed G2/G8P[4] infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-III, G8-I and G12-III, and P[4]-V and P[8]-III of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Acute gastroenteritis Rotavirus A Brazil Post-vaccine Genomic Constellation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Acute gastroenteritis Rotavirus A Brazil Post-vaccine Genomic Constellation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marcelle Silva-Sales Elcio Leal Flavio Augusto de Pádua Milagres Rafael Brustulin Vanessa dos Santos Morais Roberta Marcatti Emerson Luiz Lima Araújo Steven S. Witkin Xutao Deng Ester Cerdeira Sabino Eric Delwart Adriana Luchs Antonio Charlys da Costa Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
topic_facet |
Acute gastroenteritis Rotavirus A Brazil Post-vaccine Genomic Constellation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination follow-up (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%), as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G2P[4] (5.6%) and G8P[4] (2.8%) strains, including the mixed G2/G8P[4] infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-III, G8-I and G12-III, and P[4]-V and P[8]-III of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area. |
format |
Article in Journal/Newspaper |
author |
Marcelle Silva-Sales Elcio Leal Flavio Augusto de Pádua Milagres Rafael Brustulin Vanessa dos Santos Morais Roberta Marcatti Emerson Luiz Lima Araújo Steven S. Witkin Xutao Deng Ester Cerdeira Sabino Eric Delwart Adriana Luchs Antonio Charlys da Costa |
author_facet |
Marcelle Silva-Sales Elcio Leal Flavio Augusto de Pádua Milagres Rafael Brustulin Vanessa dos Santos Morais Roberta Marcatti Emerson Luiz Lima Araújo Steven S. Witkin Xutao Deng Ester Cerdeira Sabino Eric Delwart Adriana Luchs Antonio Charlys da Costa |
author_sort |
Marcelle Silva-Sales |
title |
Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
title_short |
Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
title_full |
Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
title_fullStr |
Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
title_full_unstemmed |
Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016) |
title_sort |
genomic constellation of human rotavirus a strains identified in northern brazil: a 6-year follow-up (2010-2016) |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2020 |
url |
https://doi.org/10.1590/s1678-9946202062098 https://doaj.org/article/2844989403704b73ba63e5218b174251 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100248&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202062098 https://doaj.org/article/2844989403704b73ba63e5218b174251 |
op_doi |
https://doi.org/10.1590/s1678-9946202062098 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
62 |
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1809897289858154496 |