Evaluation of released malathion and spinosad from chitosan/alginate/gelatin capsules against Culex pipiens larvae

• Main Authors: Badawy MEI, Taktak NEM, Awad OM, Elfiki SA, El-Ela NEA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2016
• Subjects: Chitosan capsules; Spinosad; Malathion; Controlled release formulation; Swelling; Mosquitocidal activity; Culex pipiens; Biochemical study; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doaj.org/article/282a4685c7fd4175b6e4783ffb91a75d

Mohamed EI Badawy,1 Nehad EM Taktak,2 Osama M Awad,2 Souraya A Elfiki,2 Nadia E Abou El-Ela2 1Department of Pesticide Chemistry and Technology, Faculty of Agriculture, 2Department of Tropical Health, High Institute of Public Health, Alexandria University, Alexandria, Egypt Abstract: Efficacy of spinosad and malathion loaded in eco-friendly biodegradable formulations was evaluated for controlling Culex pipiens larvae. Malathion (organophosphorus larvicide) and spinosad (naturally derived insecticide) were loaded on chitosan/alginate/gelatin capsules. Capsules were characterized by size measurement, scanning electron microscopy, Fourier transform infrared spectroscopy, and water uptake. In vitro release kinetics of the larvicides was studied in the running and stagnant water. Biochemical studies on the larvae treated with technical and formulated insecticides were also demonstrated. The results indicated that the released spinosad was active for a long time up to 48 and 211days in the running and stagnant water, respectively. However, the capsules loaded with malathion showed larvicidal activity for 20 and 27days in the running and stagnant water, respectively. Technical and formulated malathion and spinosad had an inhibition effect on acetylcholinesterase, carboxylesterase, and glutathione S-transferase. The results proved that the prepared capsules consisting of biodegradable polymers containing larvicides could be effective as controlled-release formulation against C. pipiens larvae for a long period. Keywords: chitosan capsules, larvicide, controlled-release formulation, swelling, mosquitocidal activity, Culex pipiens, biochemical study