Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments
Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for...
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ftdoajarticles:oai:doaj.org/article:28244a15715e4bf28a1c8103388a8b36 2023-05-15T16:36:59+02:00 Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli 2020-08-01T00:00:00Z https://doi.org/10.3390/microorganisms8091282 https://doaj.org/article/28244a15715e4bf28a1c8103388a8b36 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/9/1282 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091282 2076-2607 https://doaj.org/article/28244a15715e4bf28a1c8103388a8b36 Microorganisms, Vol 8, Iss 1282, p 1282 (2020) bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8091282 2022-12-31T00:58:39Z Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for isolation of microorganisms by culture-based analysis. The discovery of novel psychrophiles and their biomolecules makes these extreme environments suitable sources for the isolation of new strains, including for potential biotechnological applications. In this study, samples of bottom sediments were collected from three permafrost thaw lakes in subarctic Québec, Canada. Their diverse microbial communities were characterized by 16S rRNA gene amplicon analysis, and subsamples were cultured for the isolation of bacterial strains. Phenotypic and genetic characterization of the isolates revealed affinities to the genera Pseudomonas, Paenibacillus, Acinetobacter, Staphylococcus and Sphingomonas . The isolates were then evaluated for their production of extracellular enzymes and exopolymers. Enzymes of potential biotechnological interest included α and β-glucosidase, α and β-maltosidase, β-xylosidase and cellobiohydrolase. One isolate, Pseudomonas extremaustralis strain 2ASCA, also showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers. Article in Journal/Newspaper Ice permafrost Subarctic Thermokarst Directory of Open Access Journals: DOAJ Articles Canada Microorganisms 8 9 1282 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic Biology (General) QH301-705.5 |
spellingShingle |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic Biology (General) QH301-705.5 Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
topic_facet |
bacterial diversity exopolysaccharides lake sediments microbial ecology permafrost thaw lake subarctic Biology (General) QH301-705.5 |
description |
Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for isolation of microorganisms by culture-based analysis. The discovery of novel psychrophiles and their biomolecules makes these extreme environments suitable sources for the isolation of new strains, including for potential biotechnological applications. In this study, samples of bottom sediments were collected from three permafrost thaw lakes in subarctic Québec, Canada. Their diverse microbial communities were characterized by 16S rRNA gene amplicon analysis, and subsamples were cultured for the isolation of bacterial strains. Phenotypic and genetic characterization of the isolates revealed affinities to the genera Pseudomonas, Paenibacillus, Acinetobacter, Staphylococcus and Sphingomonas . The isolates were then evaluated for their production of extracellular enzymes and exopolymers. Enzymes of potential biotechnological interest included α and β-glucosidase, α and β-maltosidase, β-xylosidase and cellobiohydrolase. One isolate, Pseudomonas extremaustralis strain 2ASCA, also showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers. |
format |
Article in Journal/Newspaper |
author |
Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli |
author_facet |
Ilaria Finore Adrien Vigneron Warwick F. Vincent Luigi Leone Paola Di Donato Aniello Schiano Moriello Barbara Nicolaus Annarita Poli |
author_sort |
Ilaria Finore |
title |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_short |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_full |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_fullStr |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_full_unstemmed |
Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments |
title_sort |
novel psychrophiles and exopolymers from permafrost thaw lake sediments |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8091282 https://doaj.org/article/28244a15715e4bf28a1c8103388a8b36 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Ice permafrost Subarctic Thermokarst |
genre_facet |
Ice permafrost Subarctic Thermokarst |
op_source |
Microorganisms, Vol 8, Iss 1282, p 1282 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/9/1282 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8091282 2076-2607 https://doaj.org/article/28244a15715e4bf28a1c8103388a8b36 |
op_doi |
https://doi.org/10.3390/microorganisms8091282 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
9 |
container_start_page |
1282 |
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1766027302959841280 |