Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis
ABSTRACT Background: Leishmania braziliensis, a protozoan prevalent in Brazil, is the known causative agent of cutaneous leishmaniasis (CL). The activation of M1 macrophages is a pivotal factor in the host's ability to eliminate the parasite, whereas M2 macrophages may facilitate parasite proli...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2024
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Online Access: | https://doi.org/10.1590/0037-8682-0036-2024 https://doaj.org/article/2814d174737c4d2f9ab94d494162e89e |
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ftdoajarticles:oai:doaj.org/article:2814d174737c4d2f9ab94d494162e89e 2024-09-09T19:27:32+00:00 Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis Joselina Maria da Silva Helen Aguiar Lemes da Silva Ana Lucia Carneiro Sarmento Marcia Hueb Amílcar Sabino Damazo 2024-07-01T00:00:00Z https://doi.org/10.1590/0037-8682-0036-2024 https://doaj.org/article/2814d174737c4d2f9ab94d494162e89e EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822024000100412&lng=en&tlng=en http://www.scielo.br/pdf/rsbmt/v57/1678-9849-rsbmt-57-e00412-2024.pdf https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0036-2024 https://doaj.org/article/2814d174737c4d2f9ab94d494162e89e Revista da Sociedade Brasileira de Medicina Tropical, Vol 57 (2024) Leishmania braziliensis Cutaneous leishmaniasis Cytokines Annexin-A1 Histopathology Arctic medicine. Tropical medicine RC955-962 article 2024 ftdoajarticles https://doi.org/10.1590/0037-8682-0036-2024 2024-08-05T17:48:48Z ABSTRACT Background: Leishmania braziliensis, a protozoan prevalent in Brazil, is the known causative agent of cutaneous leishmaniasis (CL). The activation of M1 macrophages is a pivotal factor in the host's ability to eliminate the parasite, whereas M2 macrophages may facilitate parasite proliferation. This study analyzed the clinical outcomes of CL and the patients' immunological profiles, focusing on the prevalence of M1 and M2 macrophages, cytokine production, and annexin-A1 (ANXA1) expression in the lesion. Methods: Data were obtained by polymerase chain reaction (PCR) and histopathological, immunofluorescence, and cytokine analyses. Results: Patients with exudative and cellular reaction-type (ECR)-type lesions that healed within 90 days showed a significant increase in M1. Conversely, patients with ECR and exudative and granulomatous reaction (EGR)types, who healed within 180 days, showed an elevated number of M2. Cytokines interferon (IFN)-γ and tumor necrosis factor (TNF)-α were higher in ECR lesions that resolved within 90 days (P<0.05). In contrast, IL-9 and IL-10 levels significantly increased in both ECR and EGR lesions that healed after 180 days (P<0.001). The production of IL-21, IL-23 and TGF-β was increased in patients with ECR or EGR lesions that healed after 180 days (P<0.05). The expression of ANXA1 was higher in M2 within ECR-type lesions in patients who healed after 180 days (P<0.05). Conclusions: These findings suggest that the infectious microenvironment induced by L. braziliensis affects the differentiation of M1 and M2 macrophages, cytokine release, and ANXA1 expression, thereby influencing the healing capacity of patients. Therefore, histopathological and immunological investigations may improve the selection of CL therapy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 57 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leishmania braziliensis Cutaneous leishmaniasis Cytokines Annexin-A1 Histopathology Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Leishmania braziliensis Cutaneous leishmaniasis Cytokines Annexin-A1 Histopathology Arctic medicine. Tropical medicine RC955-962 Joselina Maria da Silva Helen Aguiar Lemes da Silva Ana Lucia Carneiro Sarmento Marcia Hueb Amílcar Sabino Damazo Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
topic_facet |
Leishmania braziliensis Cutaneous leishmaniasis Cytokines Annexin-A1 Histopathology Arctic medicine. Tropical medicine RC955-962 |
description |
ABSTRACT Background: Leishmania braziliensis, a protozoan prevalent in Brazil, is the known causative agent of cutaneous leishmaniasis (CL). The activation of M1 macrophages is a pivotal factor in the host's ability to eliminate the parasite, whereas M2 macrophages may facilitate parasite proliferation. This study analyzed the clinical outcomes of CL and the patients' immunological profiles, focusing on the prevalence of M1 and M2 macrophages, cytokine production, and annexin-A1 (ANXA1) expression in the lesion. Methods: Data were obtained by polymerase chain reaction (PCR) and histopathological, immunofluorescence, and cytokine analyses. Results: Patients with exudative and cellular reaction-type (ECR)-type lesions that healed within 90 days showed a significant increase in M1. Conversely, patients with ECR and exudative and granulomatous reaction (EGR)types, who healed within 180 days, showed an elevated number of M2. Cytokines interferon (IFN)-γ and tumor necrosis factor (TNF)-α were higher in ECR lesions that resolved within 90 days (P<0.05). In contrast, IL-9 and IL-10 levels significantly increased in both ECR and EGR lesions that healed after 180 days (P<0.001). The production of IL-21, IL-23 and TGF-β was increased in patients with ECR or EGR lesions that healed after 180 days (P<0.05). The expression of ANXA1 was higher in M2 within ECR-type lesions in patients who healed after 180 days (P<0.05). Conclusions: These findings suggest that the infectious microenvironment induced by L. braziliensis affects the differentiation of M1 and M2 macrophages, cytokine release, and ANXA1 expression, thereby influencing the healing capacity of patients. Therefore, histopathological and immunological investigations may improve the selection of CL therapy. |
format |
Article in Journal/Newspaper |
author |
Joselina Maria da Silva Helen Aguiar Lemes da Silva Ana Lucia Carneiro Sarmento Marcia Hueb Amílcar Sabino Damazo |
author_facet |
Joselina Maria da Silva Helen Aguiar Lemes da Silva Ana Lucia Carneiro Sarmento Marcia Hueb Amílcar Sabino Damazo |
author_sort |
Joselina Maria da Silva |
title |
Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
title_short |
Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
title_full |
Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
title_fullStr |
Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
title_full_unstemmed |
Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis |
title_sort |
analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-a1 in the cutaneous infection in patients with leishmania braziliensis |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2024 |
url |
https://doi.org/10.1590/0037-8682-0036-2024 https://doaj.org/article/2814d174737c4d2f9ab94d494162e89e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 57 (2024) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822024000100412&lng=en&tlng=en http://www.scielo.br/pdf/rsbmt/v57/1678-9849-rsbmt-57-e00412-2024.pdf https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0036-2024 https://doaj.org/article/2814d174737c4d2f9ab94d494162e89e |
op_doi |
https://doi.org/10.1590/0037-8682-0036-2024 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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57 |
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