Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype.
Zika virus (ZIKV) emerged as a global public health threat throughout the Americas since 2014. Phylogenetically, the virus is composed of three main lineages, an African, Asian, and American lineage. The recent emergence and spread of ZIKV has raised questions regarding the breadth and potency of hu...
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ftdoajarticles:oai:doaj.org/article:27fb6399c9ce42a391b4c42122030e64 2023-05-15T15:13:24+02:00 Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. Chad D Nix Jonathan Salberg Felicity J Coulter Bettie W Kareko Zoe L Lyski Brian L Booty William B Messer 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008006 https://doaj.org/article/27fb6399c9ce42a391b4c42122030e64 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008006 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008006 https://doaj.org/article/27fb6399c9ce42a391b4c42122030e64 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008006 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008006 2022-12-31T07:16:57Z Zika virus (ZIKV) emerged as a global public health threat throughout the Americas since 2014. Phylogenetically, the virus is composed of three main lineages, an African, Asian, and American lineage. The recent emergence and spread of ZIKV has raised questions regarding the breadth and potency of human primary ZIKV immune sera against antigenically diverse ZIKV. Although ZIKV is thought to compose a single antigenic serotype, in-depth evaluation of the antigenic relatedness of ZIKV across genetic variants has been limited to a relatively small series of early convalescent human immune sera (4-12 weeks) against a limited number (3) of genetic variants. Using virus neutralization assays, we characterize the potency and breadth of twelve primary ZIKV immune sera from adults infected 5 to 38 months previously against a panel of 11 ZIKV isolates from the African, Asian and American lineages. We assess the variability of neutralization potency of immune sera from these subjects and the variability of susceptibility to neutralization for each virus isolate. Overall, we found all sera neutralized all viruses at FRNT50 ranging from 1:271 to 1:4271, a 15.8-fold range, with only small differences between subject geometric mean titers (GMT) against all viruses and small differences between each ZIKV isolate and sensitivity to neutralization by all sera: when pooled, African strains were 1.3-fold more sensitive to neutralization by subject immune sera compared to pooled American strains. Finally, we subjected our data to analysis using antigenic cartography, finding that ZIKV are highly antigenically similar, with only a ~4-fold range across all antigenic distances between viruses, consistent with a single serotype. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008006 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Chad D Nix Jonathan Salberg Felicity J Coulter Bettie W Kareko Zoe L Lyski Brian L Booty William B Messer Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Zika virus (ZIKV) emerged as a global public health threat throughout the Americas since 2014. Phylogenetically, the virus is composed of three main lineages, an African, Asian, and American lineage. The recent emergence and spread of ZIKV has raised questions regarding the breadth and potency of human primary ZIKV immune sera against antigenically diverse ZIKV. Although ZIKV is thought to compose a single antigenic serotype, in-depth evaluation of the antigenic relatedness of ZIKV across genetic variants has been limited to a relatively small series of early convalescent human immune sera (4-12 weeks) against a limited number (3) of genetic variants. Using virus neutralization assays, we characterize the potency and breadth of twelve primary ZIKV immune sera from adults infected 5 to 38 months previously against a panel of 11 ZIKV isolates from the African, Asian and American lineages. We assess the variability of neutralization potency of immune sera from these subjects and the variability of susceptibility to neutralization for each virus isolate. Overall, we found all sera neutralized all viruses at FRNT50 ranging from 1:271 to 1:4271, a 15.8-fold range, with only small differences between subject geometric mean titers (GMT) against all viruses and small differences between each ZIKV isolate and sensitivity to neutralization by all sera: when pooled, African strains were 1.3-fold more sensitive to neutralization by subject immune sera compared to pooled American strains. Finally, we subjected our data to analysis using antigenic cartography, finding that ZIKV are highly antigenically similar, with only a ~4-fold range across all antigenic distances between viruses, consistent with a single serotype. |
format |
Article in Journal/Newspaper |
author |
Chad D Nix Jonathan Salberg Felicity J Coulter Bettie W Kareko Zoe L Lyski Brian L Booty William B Messer |
author_facet |
Chad D Nix Jonathan Salberg Felicity J Coulter Bettie W Kareko Zoe L Lyski Brian L Booty William B Messer |
author_sort |
Chad D Nix |
title |
Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
title_short |
Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
title_full |
Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
title_fullStr |
Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
title_full_unstemmed |
Potency and breadth of human primary ZIKV immune sera shows that Zika viruses cluster antigenically as a single serotype. |
title_sort |
potency and breadth of human primary zikv immune sera shows that zika viruses cluster antigenically as a single serotype. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008006 https://doaj.org/article/27fb6399c9ce42a391b4c42122030e64 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008006 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008006 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008006 https://doaj.org/article/27fb6399c9ce42a391b4c42122030e64 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008006 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
e0008006 |
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1766343954542886912 |