Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization
The present study was designed to evaluate how environmental factors in a dry-summer subtropical climate in Terceira-Azores (situated in the North Atlantic Ocean: 38° 43′ N 27° 12′ W) can affect dairy cow (Holstein) fertility, as well as seasonal influence on in vitro oocytes maturation and embryos...
Published in: | Asian-Australasian Journal of Animal Sciences |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Asian-Australasian Association of Animal Production Societies
2015
|
Subjects: | |
Online Access: | https://doi.org/10.5713/ajas.14.0480 https://doaj.org/article/27e29dcbc72e4fe9a6a7a76e5d4e3fa8 |
id |
ftdoajarticles:oai:doaj.org/article:27e29dcbc72e4fe9a6a7a76e5d4e3fa8 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:27e29dcbc72e4fe9a6a7a76e5d4e3fa8 2023-05-15T17:37:06+02:00 Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization Krishna Pavani Isabel Carvalhais Marwa Faheem Antonio Chaveiro Francisco Vieira Reis Fernando Moreira da Silva 2015-03-01T00:00:00Z https://doi.org/10.5713/ajas.14.0480 https://doaj.org/article/27e29dcbc72e4fe9a6a7a76e5d4e3fa8 EN eng Asian-Australasian Association of Animal Production Societies http://www.ajas.info/upload/pdf/ajas-28-3-334.pdf https://doaj.org/toc/1011-2367 https://doaj.org/toc/1976-5517 1011-2367 1976-5517 doi:10.5713/ajas.14.0480 https://doaj.org/article/27e29dcbc72e4fe9a6a7a76e5d4e3fa8 Asian-Australasian Journal of Animal Sciences, Vol 28, Iss 3, Pp 334-342 (2015) Environmental Stress Artificial Insemination Heat Shock Oocyte Meiotic Maturation Maternal Heat Stress Animal culture SF1-1100 Animal biochemistry QP501-801 article 2015 ftdoajarticles https://doi.org/10.5713/ajas.14.0480 2022-12-31T15:01:54Z The present study was designed to evaluate how environmental factors in a dry-summer subtropical climate in Terceira-Azores (situated in the North Atlantic Ocean: 38° 43′ N 27° 12′ W) can affect dairy cow (Holstein) fertility, as well as seasonal influence on in vitro oocytes maturation and embryos development. Impact of heat shock (HS) effects on in vitro oocyte’s maturation and further embryo development after in vitro fertilization (IVF) was also evaluated. For such purpose the result of the first artificial insemination (AI) performed 60 to 90 days after calving of 6,300 cows were recorded for one year. In parallel, climatic data was obtained at different elevation points (n = 5) from 0 to 1,000 m and grazing points from 0 to 500 m, in Terceira island, and the temperature humidity index (THI) was calculated. For in vitro experiments, oocytes (n = 706) were collected weekly during all year, for meiotic maturation and IVF. Further, to evaluate HS effect, 891 oocytes were collected in the cold moths (December, January, February and March) and divided in three groups treated to HS for 24 h during in vitro maturation at: C (Control = 38.5°C), HS1 (39.5°C) and HS2 (40.5°C). Oocytes from each group were used for meiotic assessment and IVF. Cleavage, morula and blastocyst development were evaluated respectively on day 2, 6, and 9 after IVF. A negative correlation between cow’s conception rate (CR) and THI in grazing points (−91.3%; p<0.001) was observed. Mean THI in warmer months (June, July, August and September) was 71.7±0.7 and the CR (40.2±1.5%) while in cold months THI was 62.8±0.2 and CR was 63.8±0.4%. A similar impact was obtained with in vitro results in which nuclear maturation rate (NMR) ranged from 78.4% (±8.0) to 44.3% (±8.1), while embryos development ranged from 53.8% (±5.8) to 36.3% (±3.3) in cold and warmer months respectively. In vitro HS results showed a significant decline (p<0.05) on NMR of oocytes for every 1°C rising temperature (78.4±8.0, 21.7±3.1 and 8.9±2.2, respectively for C, HS1, ... Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Asian-Australasian Journal of Animal Sciences 28 3 334 342 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Environmental Stress Artificial Insemination Heat Shock Oocyte Meiotic Maturation Maternal Heat Stress Animal culture SF1-1100 Animal biochemistry QP501-801 |
spellingShingle |
Environmental Stress Artificial Insemination Heat Shock Oocyte Meiotic Maturation Maternal Heat Stress Animal culture SF1-1100 Animal biochemistry QP501-801 Krishna Pavani Isabel Carvalhais Marwa Faheem Antonio Chaveiro Francisco Vieira Reis Fernando Moreira da Silva Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
topic_facet |
Environmental Stress Artificial Insemination Heat Shock Oocyte Meiotic Maturation Maternal Heat Stress Animal culture SF1-1100 Animal biochemistry QP501-801 |
description |
The present study was designed to evaluate how environmental factors in a dry-summer subtropical climate in Terceira-Azores (situated in the North Atlantic Ocean: 38° 43′ N 27° 12′ W) can affect dairy cow (Holstein) fertility, as well as seasonal influence on in vitro oocytes maturation and embryos development. Impact of heat shock (HS) effects on in vitro oocyte’s maturation and further embryo development after in vitro fertilization (IVF) was also evaluated. For such purpose the result of the first artificial insemination (AI) performed 60 to 90 days after calving of 6,300 cows were recorded for one year. In parallel, climatic data was obtained at different elevation points (n = 5) from 0 to 1,000 m and grazing points from 0 to 500 m, in Terceira island, and the temperature humidity index (THI) was calculated. For in vitro experiments, oocytes (n = 706) were collected weekly during all year, for meiotic maturation and IVF. Further, to evaluate HS effect, 891 oocytes were collected in the cold moths (December, January, February and March) and divided in three groups treated to HS for 24 h during in vitro maturation at: C (Control = 38.5°C), HS1 (39.5°C) and HS2 (40.5°C). Oocytes from each group were used for meiotic assessment and IVF. Cleavage, morula and blastocyst development were evaluated respectively on day 2, 6, and 9 after IVF. A negative correlation between cow’s conception rate (CR) and THI in grazing points (−91.3%; p<0.001) was observed. Mean THI in warmer months (June, July, August and September) was 71.7±0.7 and the CR (40.2±1.5%) while in cold months THI was 62.8±0.2 and CR was 63.8±0.4%. A similar impact was obtained with in vitro results in which nuclear maturation rate (NMR) ranged from 78.4% (±8.0) to 44.3% (±8.1), while embryos development ranged from 53.8% (±5.8) to 36.3% (±3.3) in cold and warmer months respectively. In vitro HS results showed a significant decline (p<0.05) on NMR of oocytes for every 1°C rising temperature (78.4±8.0, 21.7±3.1 and 8.9±2.2, respectively for C, HS1, ... |
format |
Article in Journal/Newspaper |
author |
Krishna Pavani Isabel Carvalhais Marwa Faheem Antonio Chaveiro Francisco Vieira Reis Fernando Moreira da Silva |
author_facet |
Krishna Pavani Isabel Carvalhais Marwa Faheem Antonio Chaveiro Francisco Vieira Reis Fernando Moreira da Silva |
author_sort |
Krishna Pavani |
title |
Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
title_short |
Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
title_full |
Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
title_fullStr |
Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
title_full_unstemmed |
Reproductive Performance of Holstein Dairy Cows Grazing in Dry-summer Subtropical Climatic Conditions: Effect of Heat Stress and Heat Shock on Meiotic Competence and Fertilization |
title_sort |
reproductive performance of holstein dairy cows grazing in dry-summer subtropical climatic conditions: effect of heat stress and heat shock on meiotic competence and fertilization |
publisher |
Asian-Australasian Association of Animal Production Societies |
publishDate |
2015 |
url |
https://doi.org/10.5713/ajas.14.0480 https://doaj.org/article/27e29dcbc72e4fe9a6a7a76e5d4e3fa8 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Asian-Australasian Journal of Animal Sciences, Vol 28, Iss 3, Pp 334-342 (2015) |
op_relation |
http://www.ajas.info/upload/pdf/ajas-28-3-334.pdf https://doaj.org/toc/1011-2367 https://doaj.org/toc/1976-5517 1011-2367 1976-5517 doi:10.5713/ajas.14.0480 https://doaj.org/article/27e29dcbc72e4fe9a6a7a76e5d4e3fa8 |
op_doi |
https://doi.org/10.5713/ajas.14.0480 |
container_title |
Asian-Australasian Journal of Animal Sciences |
container_volume |
28 |
container_issue |
3 |
container_start_page |
334 |
op_container_end_page |
342 |
_version_ |
1766136828913516544 |