Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea
Abstract Background Malaria is still a serious public health problem on Bioko Island (Equatorial Guinea), although the number of annual cases has been greatly reduced since 2004 through the Bioko Island Malaria Control Project (BIMCP). A better understanding of malaria parasite population diversity...
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ftdoajarticles:oai:doaj.org/article:27d660c7865747e4bff1877de0de7274 2023-05-15T15:18:15+02:00 Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea Jiang-Tao Chen Jian Li Guang-Cai Zha Guang Huang Zhi-Xiu Huang Dong-De Xie Xia Zhou Huan-Tong Mo Juan Urbano Monsuy Eyi Rocio Apicante Matesa Maximo Miko Ondo Obono Shan Li Xiang-Zhi Liu Min Lin 2018-12-01T00:00:00Z https://doi.org/10.1186/s12936-018-2611-z https://doaj.org/article/27d660c7865747e4bff1877de0de7274 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2611-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2611-z 1475-2875 https://doaj.org/article/27d660c7865747e4bff1877de0de7274 Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) Plasmodium falciparum Bioko Island Genetic diversity Multiplicity of infection (MOI) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2611-z 2022-12-31T12:17:58Z Abstract Background Malaria is still a serious public health problem on Bioko Island (Equatorial Guinea), although the number of annual cases has been greatly reduced since 2004 through the Bioko Island Malaria Control Project (BIMCP). A better understanding of malaria parasite population diversity and transmission dynamics is critical for assessing the effectiveness of malaria control measures. The objective of this study is to investigate the genetic diversity of Plasmodium falciparum populations and multiplicity of infection (MOI) on Bioko Island 7 years after BIMCP. Methods A total of 181 patients with uncomplicated P. falciparum malaria diagnosed with microscopy were collected from Bioko Island from January 2011 to December 2014. Parasite DNA was extracted using chelex-100 and species were identified using a real-time PCR followed by high-resolution melting. Plasmodium falciparum msp1 and msp2 allelic families were determined using nested PCR. Results Three msp1 alleles (K1, MAD20, and RO33) and two msp2 alleles (FC27 and 3D7) were analysed in all samples. In msp1, the MAD20 allelic family was predominant with 96.69% (175/178) followed respectively by the K1 allelic family with 96.07% (171/178) and R033 allelic family with 70.78% (126/178). In msp2, the FC27 allelic family was the most frequently detected with 97.69% (169/173) compared to 3D7 with 72.25% (125/173). Twenty-six different alleles were observed in msp1 with 9 alleles for K1, 9 alleles for MAD20 and 8 alleles for R033. In msp2, 25 individual alleles were detected with 5 alleles for FC27 and 20 alleles for 3D7. The overall MOI was 5.51 with respectively 3.5 and 2.01 for msp1 and msp2. A significant increase in overall MOI was correlated with the age group of the patients (P = 0.026) or parasite densities (P = 0.04). Conclusions The present data showed high genetic diversity and MOI values among the P. falciparum population in the study, reflecting both the high endemic level and malaria transmission on Bioko Island. These data provide valuable ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
Plasmodium falciparum Bioko Island Genetic diversity Multiplicity of infection (MOI) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Bioko Island Genetic diversity Multiplicity of infection (MOI) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jiang-Tao Chen Jian Li Guang-Cai Zha Guang Huang Zhi-Xiu Huang Dong-De Xie Xia Zhou Huan-Tong Mo Juan Urbano Monsuy Eyi Rocio Apicante Matesa Maximo Miko Ondo Obono Shan Li Xiang-Zhi Liu Min Lin Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
topic_facet |
Plasmodium falciparum Bioko Island Genetic diversity Multiplicity of infection (MOI) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is still a serious public health problem on Bioko Island (Equatorial Guinea), although the number of annual cases has been greatly reduced since 2004 through the Bioko Island Malaria Control Project (BIMCP). A better understanding of malaria parasite population diversity and transmission dynamics is critical for assessing the effectiveness of malaria control measures. The objective of this study is to investigate the genetic diversity of Plasmodium falciparum populations and multiplicity of infection (MOI) on Bioko Island 7 years after BIMCP. Methods A total of 181 patients with uncomplicated P. falciparum malaria diagnosed with microscopy were collected from Bioko Island from January 2011 to December 2014. Parasite DNA was extracted using chelex-100 and species were identified using a real-time PCR followed by high-resolution melting. Plasmodium falciparum msp1 and msp2 allelic families were determined using nested PCR. Results Three msp1 alleles (K1, MAD20, and RO33) and two msp2 alleles (FC27 and 3D7) were analysed in all samples. In msp1, the MAD20 allelic family was predominant with 96.69% (175/178) followed respectively by the K1 allelic family with 96.07% (171/178) and R033 allelic family with 70.78% (126/178). In msp2, the FC27 allelic family was the most frequently detected with 97.69% (169/173) compared to 3D7 with 72.25% (125/173). Twenty-six different alleles were observed in msp1 with 9 alleles for K1, 9 alleles for MAD20 and 8 alleles for R033. In msp2, 25 individual alleles were detected with 5 alleles for FC27 and 20 alleles for 3D7. The overall MOI was 5.51 with respectively 3.5 and 2.01 for msp1 and msp2. A significant increase in overall MOI was correlated with the age group of the patients (P = 0.026) or parasite densities (P = 0.04). Conclusions The present data showed high genetic diversity and MOI values among the P. falciparum population in the study, reflecting both the high endemic level and malaria transmission on Bioko Island. These data provide valuable ... |
format |
Article in Journal/Newspaper |
author |
Jiang-Tao Chen Jian Li Guang-Cai Zha Guang Huang Zhi-Xiu Huang Dong-De Xie Xia Zhou Huan-Tong Mo Juan Urbano Monsuy Eyi Rocio Apicante Matesa Maximo Miko Ondo Obono Shan Li Xiang-Zhi Liu Min Lin |
author_facet |
Jiang-Tao Chen Jian Li Guang-Cai Zha Guang Huang Zhi-Xiu Huang Dong-De Xie Xia Zhou Huan-Tong Mo Juan Urbano Monsuy Eyi Rocio Apicante Matesa Maximo Miko Ondo Obono Shan Li Xiang-Zhi Liu Min Lin |
author_sort |
Jiang-Tao Chen |
title |
Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
title_short |
Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
title_full |
Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
title_fullStr |
Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
title_full_unstemmed |
Genetic diversity and allele frequencies of Plasmodium falciparum msp1 and msp2 in parasite isolates from Bioko Island, Equatorial Guinea |
title_sort |
genetic diversity and allele frequencies of plasmodium falciparum msp1 and msp2 in parasite isolates from bioko island, equatorial guinea |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2611-z https://doaj.org/article/27d660c7865747e4bff1877de0de7274 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-9 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2611-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2611-z 1475-2875 https://doaj.org/article/27d660c7865747e4bff1877de0de7274 |
op_doi |
https://doi.org/10.1186/s12936-018-2611-z |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766348454261424128 |