Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection
IntroductionProphylactic vaccination is regarded as the most effective means to control avian flu infection. Currently, there is a need for a universal vaccine that provides broad and long-lasting protection against influenza virus. Meanwhile, although yeast-based vaccines have been used in clinic,...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fmicb.2023.1153922 https://doaj.org/article/27cab774f0ac4422ab171d09d91f0291 |
id |
ftdoajarticles:oai:doaj.org/article:27cab774f0ac4422ab171d09d91f0291 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:27cab774f0ac4422ab171d09d91f0291 2023-06-18T03:39:54+02:00 Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection Han Zhang Zexing Li Huixia Zhang Yanyu Guo Xinyi Zhang Lilin Zhang Liu Yang Shujun Li Changyan Li Daqing Cui Ruyu Xie Yongqing Li Jinhai Huang 2023-05-01T00:00:00Z https://doi.org/10.3389/fmicb.2023.1153922 https://doaj.org/article/27cab774f0ac4422ab171d09d91f0291 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153922/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1153922 https://doaj.org/article/27cab774f0ac4422ab171d09d91f0291 Frontiers in Microbiology, Vol 14 (2023) avian influenza virus Saccharomyces cerevisiae innate immunity vaccine chicken Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.3389/fmicb.2023.1153922 2023-06-04T00:37:55Z IntroductionProphylactic vaccination is regarded as the most effective means to control avian flu infection. Currently, there is a need for a universal vaccine that provides broad and long-lasting protection against influenza virus. Meanwhile, although yeast-based vaccines have been used in clinic, studies are still required to further understand the molecular mechanism of yeast-based vaccines under physiological conditions.MethodsWe generated a yeast-based vaccine against influenza hemagglutinin (HA) of H5, H7 and H9 using surface displaying technology and evaluated the protective efficacy of chickens after exposure to H9N2 influenza virus.ResultsOral yeast vaccine provided less clinical syndrome, reduced viral loading and alleviated airway damage significantly. Compared to the commercial inactivated vaccine, yeast vaccine stimulated the activation of splenic NK and APCs cells and boosted TLR7-IRF7-IFN signaling in spleen. Meanwhile, γδ T cells in the bursa of Fabricius were activated and the innate lymphoid cells (ILCs) in the bursa of Fabricius promoted the CILPs to differentiate to ILC3 cells in oral yeast birds. Moreover, the reshaped gut microbiota and a suppressed Th17-IL17-mediated inflammation in intestine was observed in oral yeast chickens, which might facilitate the recovery of intestinal mucosal immunity upon virus infection. Collectively, our findings suggest that oral yeast based multivalent bird flu vaccines provide an attractive strategy to update host defense function via reshapes of multi-systemic immune homeostasis. Article in Journal/Newspaper Avian flu Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 14 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
avian influenza virus Saccharomyces cerevisiae innate immunity vaccine chicken Microbiology QR1-502 |
spellingShingle |
avian influenza virus Saccharomyces cerevisiae innate immunity vaccine chicken Microbiology QR1-502 Han Zhang Zexing Li Huixia Zhang Yanyu Guo Xinyi Zhang Lilin Zhang Liu Yang Shujun Li Changyan Li Daqing Cui Ruyu Xie Yongqing Li Jinhai Huang Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
topic_facet |
avian influenza virus Saccharomyces cerevisiae innate immunity vaccine chicken Microbiology QR1-502 |
description |
IntroductionProphylactic vaccination is regarded as the most effective means to control avian flu infection. Currently, there is a need for a universal vaccine that provides broad and long-lasting protection against influenza virus. Meanwhile, although yeast-based vaccines have been used in clinic, studies are still required to further understand the molecular mechanism of yeast-based vaccines under physiological conditions.MethodsWe generated a yeast-based vaccine against influenza hemagglutinin (HA) of H5, H7 and H9 using surface displaying technology and evaluated the protective efficacy of chickens after exposure to H9N2 influenza virus.ResultsOral yeast vaccine provided less clinical syndrome, reduced viral loading and alleviated airway damage significantly. Compared to the commercial inactivated vaccine, yeast vaccine stimulated the activation of splenic NK and APCs cells and boosted TLR7-IRF7-IFN signaling in spleen. Meanwhile, γδ T cells in the bursa of Fabricius were activated and the innate lymphoid cells (ILCs) in the bursa of Fabricius promoted the CILPs to differentiate to ILC3 cells in oral yeast birds. Moreover, the reshaped gut microbiota and a suppressed Th17-IL17-mediated inflammation in intestine was observed in oral yeast chickens, which might facilitate the recovery of intestinal mucosal immunity upon virus infection. Collectively, our findings suggest that oral yeast based multivalent bird flu vaccines provide an attractive strategy to update host defense function via reshapes of multi-systemic immune homeostasis. |
format |
Article in Journal/Newspaper |
author |
Han Zhang Zexing Li Huixia Zhang Yanyu Guo Xinyi Zhang Lilin Zhang Liu Yang Shujun Li Changyan Li Daqing Cui Ruyu Xie Yongqing Li Jinhai Huang |
author_facet |
Han Zhang Zexing Li Huixia Zhang Yanyu Guo Xinyi Zhang Lilin Zhang Liu Yang Shujun Li Changyan Li Daqing Cui Ruyu Xie Yongqing Li Jinhai Huang |
author_sort |
Han Zhang |
title |
Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
title_short |
Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
title_full |
Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
title_fullStr |
Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
title_full_unstemmed |
Recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
title_sort |
recombinant hemagglutinin displaying on yeast reshapes congenital lymphocyte subsets to prompt optimized systemic immune protection against avian influenza infection |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmicb.2023.1153922 https://doaj.org/article/27cab774f0ac4422ab171d09d91f0291 |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
Frontiers in Microbiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1153922/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2023.1153922 https://doaj.org/article/27cab774f0ac4422ab171d09d91f0291 |
op_doi |
https://doi.org/10.3389/fmicb.2023.1153922 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
_version_ |
1769004697820069888 |