Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change
High Arctic meromictic lakes are extreme environments characterized by cold temperatures, low nutrient inputs from their polar desert catchments and prolonged periods of low irradiance and darkness. These lakes are permanently stratified with an oxygenated freshwater layer (mixolimnion) overlying a...
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ftdoajarticles:oai:doaj.org/article:27b0e33122c44e80bed235807255762d 2023-05-15T14:52:01+02:00 Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change Sophie eCharvet Warwick F Vincent André M Comeau Connie eLovejoy 2012-12-01T00:00:00Z https://doi.org/10.3389/fmicb.2012.00422 https://doaj.org/article/27b0e33122c44e80bed235807255762d EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00422/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2012.00422 https://doaj.org/article/27b0e33122c44e80bed235807255762d Frontiers in Microbiology, Vol 3 (2012) Climate Change Phytoplankton Protists anoxic Arctic meromictic Microbiology QR1-502 article 2012 ftdoajarticles https://doi.org/10.3389/fmicb.2012.00422 2022-12-31T13:35:08Z High Arctic meromictic lakes are extreme environments characterized by cold temperatures, low nutrient inputs from their polar desert catchments and prolonged periods of low irradiance and darkness. These lakes are permanently stratified with an oxygenated freshwater layer (mixolimnion) overlying a saline, anoxic water column (monimolimnion). The physical and chemical properties of the deepest known lake of this type in the circumpolar Arctic, Lake A, on the far northern coast of Ellesmere Island, Canada, have been studied over the last 15 years, but little is known about the lake’s biological communities. We applied high-throughput sequencing of the V4 region of the 18S ribosomal RNA gene to investigate the protist communities down the water column at three sampling times: under the ice at the end of winter in 2008, during an unusual period of warming and ice-out the same year, and again under the ice in mid-summer 2009. Sequences of many protist taxa occurred throughout the water column at all sampling times, including in the deep anoxic layer where growth is highly unlikely. Furthermore, there were sequences for taxonomic groups including diatoms and marine taxa, which have never been observed in Lake A by microscopic analysis. However the sequences of other taxa such as ciliates, chrysophytes, Cercozoa and Telonema varied with depth, between years and during the transition to ice-free conditions. These results imply that there are seasonally active taxa in the surface waters of the lake that are sensitive to depth and change with time. DNA from these taxa is superimposed upon background DNA from multiple internal and external sources that is preserved in the deep, cold, largely anoxic water column. Article in Journal/Newspaper Arctic Climate change Ellesmere Island Phytoplankton polar desert Directory of Open Access Journals: DOAJ Articles Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Canada Ellesmere Island Frontiers in Microbiology 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Climate Change Phytoplankton Protists anoxic Arctic meromictic Microbiology QR1-502 |
spellingShingle |
Climate Change Phytoplankton Protists anoxic Arctic meromictic Microbiology QR1-502 Sophie eCharvet Warwick F Vincent André M Comeau Connie eLovejoy Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
topic_facet |
Climate Change Phytoplankton Protists anoxic Arctic meromictic Microbiology QR1-502 |
description |
High Arctic meromictic lakes are extreme environments characterized by cold temperatures, low nutrient inputs from their polar desert catchments and prolonged periods of low irradiance and darkness. These lakes are permanently stratified with an oxygenated freshwater layer (mixolimnion) overlying a saline, anoxic water column (monimolimnion). The physical and chemical properties of the deepest known lake of this type in the circumpolar Arctic, Lake A, on the far northern coast of Ellesmere Island, Canada, have been studied over the last 15 years, but little is known about the lake’s biological communities. We applied high-throughput sequencing of the V4 region of the 18S ribosomal RNA gene to investigate the protist communities down the water column at three sampling times: under the ice at the end of winter in 2008, during an unusual period of warming and ice-out the same year, and again under the ice in mid-summer 2009. Sequences of many protist taxa occurred throughout the water column at all sampling times, including in the deep anoxic layer where growth is highly unlikely. Furthermore, there were sequences for taxonomic groups including diatoms and marine taxa, which have never been observed in Lake A by microscopic analysis. However the sequences of other taxa such as ciliates, chrysophytes, Cercozoa and Telonema varied with depth, between years and during the transition to ice-free conditions. These results imply that there are seasonally active taxa in the surface waters of the lake that are sensitive to depth and change with time. DNA from these taxa is superimposed upon background DNA from multiple internal and external sources that is preserved in the deep, cold, largely anoxic water column. |
format |
Article in Journal/Newspaper |
author |
Sophie eCharvet Warwick F Vincent André M Comeau Connie eLovejoy |
author_facet |
Sophie eCharvet Warwick F Vincent André M Comeau Connie eLovejoy |
author_sort |
Sophie eCharvet |
title |
Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
title_short |
Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
title_full |
Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
title_fullStr |
Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
title_full_unstemmed |
Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change |
title_sort |
pyrosequencing analysis of the protist communities in a high arctic meromictic lake: dna preservation and change |
publisher |
Frontiers Media S.A. |
publishDate |
2012 |
url |
https://doi.org/10.3389/fmicb.2012.00422 https://doaj.org/article/27b0e33122c44e80bed235807255762d |
long_lat |
ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Arctic Arctic Lake Canada Ellesmere Island |
geographic_facet |
Arctic Arctic Lake Canada Ellesmere Island |
genre |
Arctic Climate change Ellesmere Island Phytoplankton polar desert |
genre_facet |
Arctic Climate change Ellesmere Island Phytoplankton polar desert |
op_source |
Frontiers in Microbiology, Vol 3 (2012) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00422/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2012.00422 https://doaj.org/article/27b0e33122c44e80bed235807255762d |
op_doi |
https://doi.org/10.3389/fmicb.2012.00422 |
container_title |
Frontiers in Microbiology |
container_volume |
3 |
_version_ |
1766323143014612992 |