Serovar diversity of pathogenic Leptospira circulating in the French West Indies.
BACKGROUND: Leptospirosis is one of the most important neglected tropical bacterial diseases in Latin America and the Caribbean. However, very little is known about the circulating etiological agents of leptospirosis in this region. In this study, we describe the serological and molecular features o...
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ftdoajarticles:oai:doaj.org/article:2773ad25ae7048408e4aa1ae712e6ef5 2023-05-15T15:10:11+02:00 Serovar diversity of pathogenic Leptospira circulating in the French West Indies. Pascale Bourhy Cécile Herrmann Storck Rafaelle Theodose Claude Olive Muriel Nicolas Patrick Hochedez Isabelle Lamaury Farida Zinini Sylvie Brémont Annie Landier Sylvie Cassadou Jacques Rosine Mathieu Picardeau 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002114 https://doaj.org/article/2773ad25ae7048408e4aa1ae712e6ef5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3597474?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002114 https://doaj.org/article/2773ad25ae7048408e4aa1ae712e6ef5 PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2114 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002114 2022-12-31T11:16:36Z BACKGROUND: Leptospirosis is one of the most important neglected tropical bacterial diseases in Latin America and the Caribbean. However, very little is known about the circulating etiological agents of leptospirosis in this region. In this study, we describe the serological and molecular features of leptospires isolated from 104 leptospirosis patients in Guadeloupe (n = 85) and Martinique (n = 19) and six rats captured in Guadeloupe, between 2004 and 2012. METHODS AND FINDINGS: Strains were studied by serogrouping, PFGE, MLVA, and sequencing 16SrRNA and secY. DNA extracts from blood samples collected from 36 patients in Martinique were also used for molecular typing of leptospires via PCR. Phylogenetic analyses revealed thirteen different genotypes clustered into five main clades that corresponded to the species: L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchi, and L. santarosai. We also identified L. kmetyi in at least two patients with acute leptospirosis. This is the first time, to our knowledge, that this species has been identified in humans. The most prevalent genotypes were associated with L. interrogans serovars Icterohaemorrhagiae and Copenhageni, L. kirschneri serovar Bogvere, and L. borgpetersenii serovar Arborea. We were unable to identify nine strains at the serovar level and comparison of genotyping results to the MLST database revealed new secY alleles. CONCLUSIONS: The overall serovar distribution in the French West Indies was unique compared to the neighboring islands. Typing of leptospiral isolates also suggested the existence of previously undescribed serovars. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 3 e2114 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pascale Bourhy Cécile Herrmann Storck Rafaelle Theodose Claude Olive Muriel Nicolas Patrick Hochedez Isabelle Lamaury Farida Zinini Sylvie Brémont Annie Landier Sylvie Cassadou Jacques Rosine Mathieu Picardeau Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Leptospirosis is one of the most important neglected tropical bacterial diseases in Latin America and the Caribbean. However, very little is known about the circulating etiological agents of leptospirosis in this region. In this study, we describe the serological and molecular features of leptospires isolated from 104 leptospirosis patients in Guadeloupe (n = 85) and Martinique (n = 19) and six rats captured in Guadeloupe, between 2004 and 2012. METHODS AND FINDINGS: Strains were studied by serogrouping, PFGE, MLVA, and sequencing 16SrRNA and secY. DNA extracts from blood samples collected from 36 patients in Martinique were also used for molecular typing of leptospires via PCR. Phylogenetic analyses revealed thirteen different genotypes clustered into five main clades that corresponded to the species: L. interrogans, L. kirschneri, L. borgpetersenii, L. noguchi, and L. santarosai. We also identified L. kmetyi in at least two patients with acute leptospirosis. This is the first time, to our knowledge, that this species has been identified in humans. The most prevalent genotypes were associated with L. interrogans serovars Icterohaemorrhagiae and Copenhageni, L. kirschneri serovar Bogvere, and L. borgpetersenii serovar Arborea. We were unable to identify nine strains at the serovar level and comparison of genotyping results to the MLST database revealed new secY alleles. CONCLUSIONS: The overall serovar distribution in the French West Indies was unique compared to the neighboring islands. Typing of leptospiral isolates also suggested the existence of previously undescribed serovars. |
format |
Article in Journal/Newspaper |
author |
Pascale Bourhy Cécile Herrmann Storck Rafaelle Theodose Claude Olive Muriel Nicolas Patrick Hochedez Isabelle Lamaury Farida Zinini Sylvie Brémont Annie Landier Sylvie Cassadou Jacques Rosine Mathieu Picardeau |
author_facet |
Pascale Bourhy Cécile Herrmann Storck Rafaelle Theodose Claude Olive Muriel Nicolas Patrick Hochedez Isabelle Lamaury Farida Zinini Sylvie Brémont Annie Landier Sylvie Cassadou Jacques Rosine Mathieu Picardeau |
author_sort |
Pascale Bourhy |
title |
Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
title_short |
Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
title_full |
Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
title_fullStr |
Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
title_full_unstemmed |
Serovar diversity of pathogenic Leptospira circulating in the French West Indies. |
title_sort |
serovar diversity of pathogenic leptospira circulating in the french west indies. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002114 https://doaj.org/article/2773ad25ae7048408e4aa1ae712e6ef5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2114 (2013) |
op_relation |
http://europepmc.org/articles/PMC3597474?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002114 https://doaj.org/article/2773ad25ae7048408e4aa1ae712e6ef5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002114 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
3 |
container_start_page |
e2114 |
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1766341229005504512 |