Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line
Objective: To investigate the influence of Notch signaling on osteoprotegerin (OPG) expression in a human oral squamous cell carcinoma cell line. Methods: Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a γ-secretase inhibitor was a...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:275dd79661f0483995bbbcb943ed4ebf 2023-05-15T15:08:38+02:00 Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant 2016-08-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.06.012 https://doaj.org/article/275dd79661f0483995bbbcb943ed4ebf EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115309151 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.012 https://doaj.org/article/275dd79661f0483995bbbcb943ed4ebf Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 692-697 (2016) Notch signaling Oral squamous cell carcinoma Osteoprotegerin DAPT Jagged1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.06.012 2022-12-31T13:09:22Z Objective: To investigate the influence of Notch signaling on osteoprotegerin (OPG) expression in a human oral squamous cell carcinoma cell line. Methods: Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a γ-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG mRNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software. Results: Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 mRNA expression, confirming the intracellular activation of Notch signaling. OPG mRNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a γ-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 mRNA levels, and a marked increase in OPG protein expression was observed. These results implied that Notch signaling regulated OPG expression in HSC-4 cells. However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line (HSC-5) or a human head and neck squamous cell carcinoma cell line (HN22). Conclusions: Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 8 692 697 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Notch signaling Oral squamous cell carcinoma Osteoprotegerin DAPT Jagged1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Notch signaling Oral squamous cell carcinoma Osteoprotegerin DAPT Jagged1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
topic_facet |
Notch signaling Oral squamous cell carcinoma Osteoprotegerin DAPT Jagged1 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the influence of Notch signaling on osteoprotegerin (OPG) expression in a human oral squamous cell carcinoma cell line. Methods: Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a γ-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG mRNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software. Results: Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 mRNA expression, confirming the intracellular activation of Notch signaling. OPG mRNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a γ-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 mRNA levels, and a marked increase in OPG protein expression was observed. These results implied that Notch signaling regulated OPG expression in HSC-4 cells. However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line (HSC-5) or a human head and neck squamous cell carcinoma cell line (HN22). Conclusions: Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific. |
format |
Article in Journal/Newspaper |
author |
Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant |
author_facet |
Jeeranan Manokawinchoke Thanaphum Osathanon Prasit Pavasant |
author_sort |
Jeeranan Manokawinchoke |
title |
Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
title_short |
Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
title_full |
Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
title_fullStr |
Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
title_full_unstemmed |
Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line |
title_sort |
regulation of osteoprotegerin expression by notch signaling in human oral squamous cell carcinoma cell line |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.06.012 https://doaj.org/article/275dd79661f0483995bbbcb943ed4ebf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 692-697 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115309151 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.012 https://doaj.org/article/275dd79661f0483995bbbcb943ed4ebf |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.06.012 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
8 |
container_start_page |
692 |
op_container_end_page |
697 |
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1766339952452304896 |