Microimmunodiffusion test for the serodiagnosis of paracoccidioidomycosis

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Maria Isabel Correa da Silva, Luiz Gastão Chamma, Marcello Franco
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1989
• Subjects: Paracoccidioidomicose; Microimunodifusão; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651989000100008; https://doaj.org/article/2757b47a4d1f4c99a984f4bcd43841d3

We used the micro- and macroimmunodiffusion test for the qualitative and quantitative measurement of anti - P. brasiliensis antibodies in serum of patients with paracoccidioidomycosis. All 103 paracoccidioidomycosis sera (100%) were positive in the micro test versus 87% positivity index in the macrotest. All 83 control sera from patients with other diseases were negative in both tests. Titers of the positive sera tended to be higher in the microtest, which revealed sharper and easier to read precipiting bands. Microimmunodiffusion is simple to be performed, requires a minimum amount of reagents and allows the simultaneous testing of 102 sera. It may replace the macrotest specially in laboratories dealing with great serologic routine.