Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study.
Background Leptospirosis is a widespread zoonosis with global impact, particularly among vulnerable populations in resource-poor settings in tropical countries. Rodents have been considered to be the main reservoir of the disease; however, a wide variety of mammals can act as hosts as well. Here we...
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ftdoajarticles:oai:doaj.org/article:274edff3b8144f2f8d6bdf089878e2eb 2023-05-15T15:16:25+02:00 Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. Linda Grillová Hilde Angermeier Marc Levy Marine Giard Stéphane Lastère Mathieu Picardeau 2020-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008662 https://doaj.org/article/274edff3b8144f2f8d6bdf089878e2eb EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008662 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008662 https://doaj.org/article/274edff3b8144f2f8d6bdf089878e2eb PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008662 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008662 2022-12-31T10:07:33Z Background Leptospirosis is a widespread zoonosis with global impact, particularly among vulnerable populations in resource-poor settings in tropical countries. Rodents have been considered to be the main reservoir of the disease; however, a wide variety of mammals can act as hosts as well. Here we examine the genetic diversity of Leptospira strains from biological samples of patients and animals in French Polynesia (FP) from 2011 to 2019. Methodology/principal findings From 2011 to 2019, we have collected 444 blood samples from patients diagnosed as having leptospirosis. The limited volume of clinical material and low amount of leptospiral DNA in blood samples led us to develop a nested PCR targeting the secY locus that enabled us to amplify and sequence 244 samples (55%). In addition, 20 Leptospira strains recovered from the blood of patients from 2002 to 2011 were sequenced and fully characterized at the serogroup level and used as reference strains for the association of different phylogenetic branches with respective serogroups. The secY sequences were compared with publicly available sequences from patients and animal reservoirs in FP (n = 79). We identified rats as the main source of infection for L. borgpetersenii serogroup Ballum and L. interrogans serogroup Icterohaemorrhagiae, dogs as the main source of infection for L. interrogans serogroup Australis, and farm pigs as the main source of infection for L. interrogans serogroups Pomona or Canicola. L. interrogans was associated with the most severe infections with 10 and 5 fatal cases due to serogroups Icterohaemorrhagiae and Australis, respectively. Mortality was significantly associated with older age (p-value < 0.001). Conclusions/significance We described the population dynamics of leptospires circulating among patients in FP, including two patients who were reinfected with unrelated Leptospira genotypes, and clarified the local role of the animal reservoirs in the transmission route of leptospirosis to humans. Routine Leptospira genotyping ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pomona ENVELOPE(-45.900,-45.900,-60.583,-60.583) PLOS Neglected Tropical Diseases 14 9 e0008662 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Linda Grillová Hilde Angermeier Marc Levy Marine Giard Stéphane Lastère Mathieu Picardeau Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Leptospirosis is a widespread zoonosis with global impact, particularly among vulnerable populations in resource-poor settings in tropical countries. Rodents have been considered to be the main reservoir of the disease; however, a wide variety of mammals can act as hosts as well. Here we examine the genetic diversity of Leptospira strains from biological samples of patients and animals in French Polynesia (FP) from 2011 to 2019. Methodology/principal findings From 2011 to 2019, we have collected 444 blood samples from patients diagnosed as having leptospirosis. The limited volume of clinical material and low amount of leptospiral DNA in blood samples led us to develop a nested PCR targeting the secY locus that enabled us to amplify and sequence 244 samples (55%). In addition, 20 Leptospira strains recovered from the blood of patients from 2002 to 2011 were sequenced and fully characterized at the serogroup level and used as reference strains for the association of different phylogenetic branches with respective serogroups. The secY sequences were compared with publicly available sequences from patients and animal reservoirs in FP (n = 79). We identified rats as the main source of infection for L. borgpetersenii serogroup Ballum and L. interrogans serogroup Icterohaemorrhagiae, dogs as the main source of infection for L. interrogans serogroup Australis, and farm pigs as the main source of infection for L. interrogans serogroups Pomona or Canicola. L. interrogans was associated with the most severe infections with 10 and 5 fatal cases due to serogroups Icterohaemorrhagiae and Australis, respectively. Mortality was significantly associated with older age (p-value < 0.001). Conclusions/significance We described the population dynamics of leptospires circulating among patients in FP, including two patients who were reinfected with unrelated Leptospira genotypes, and clarified the local role of the animal reservoirs in the transmission route of leptospirosis to humans. Routine Leptospira genotyping ... |
format |
Article in Journal/Newspaper |
author |
Linda Grillová Hilde Angermeier Marc Levy Marine Giard Stéphane Lastère Mathieu Picardeau |
author_facet |
Linda Grillová Hilde Angermeier Marc Levy Marine Giard Stéphane Lastère Mathieu Picardeau |
author_sort |
Linda Grillová |
title |
Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
title_short |
Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
title_full |
Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
title_fullStr |
Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
title_full_unstemmed |
Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study. |
title_sort |
circulating genotypes of leptospira in french polynesia : an 9-year molecular epidemiology surveillance follow-up study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008662 https://doaj.org/article/274edff3b8144f2f8d6bdf089878e2eb |
long_lat |
ENVELOPE(-45.900,-45.900,-60.583,-60.583) |
geographic |
Arctic Pomona |
geographic_facet |
Arctic Pomona |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008662 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008662 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008662 https://doaj.org/article/274edff3b8144f2f8d6bdf089878e2eb |
op_doi |
https://doi.org/10.1371/journal.pntd.0008662 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
9 |
container_start_page |
e0008662 |
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1766346713251971072 |