Potencial de biocatálise enantiosseletiva de lipases microbianas Potential of enantioselective biocatalysis by microbial lipases

• Published in: Química Nova
• Main Authors: Patrícia de O. Carvalho, Silvana Ap. Calafatti, Maurício Marassi, Daniela M. da Silva, Fabiano J. Contesini, Renato Bizaco, Gabriela Alves Macedo
• Format: Article in Journal/Newspaper
• Language: English; Spanish; Portuguese
• Published: Sociedade Brasileira de Química 2005
• Subjects: lipases; enantioselectivity; (R,S)-ibuprofen; Chemistry; QD1-999; Antarc*; Antarctica
• Online Access: https://doi.org/10.1590/S0100-40422005000400012; https://doaj.org/article/2727939bafd245caaeb4b27471ddbee4

Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate. Three microbial lipases isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of their stability and as biocatalysts in the enantioselective esterification using racemic substrates in organic medium. The lipase from Aspergillus niger showed the highest activity (18.2 U/mL) and was highly thermostable, retaining 90% and 60% activity at 50 ºC and 60 ºC after 1 hour, respectively. In organic medium, this lipase provided the best results in terms of enantiomeric excess of the (S)-active acid (ee = 6.1%) and conversion value (c = 20%) in the esterification of (R,S)-ibuprofen with 1-propanol in isooctane. The esterification reaction of the racemic mixture of (R,S)-2-octanol with decanoic acid proceeded with high enantioselectivity when lipase from Aspergillus niger (E = 13.2) and commercial lipase from Candida antarctica (E = 20) were employed.