MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil.
Cryptococcosis is an important fungal infection in immunocompromised individuals, especially those infected with HIV. In Brazil, despite the free availability of antiretroviral therapy (ART) in the public health system, the mortality rate due to Cryptococcus neoformans meningitis is still high. To o...
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ftdoajarticles:oai:doaj.org/article:26e9412f0b634d0b8c08d7fa8841f6d2 2023-05-15T15:11:20+02:00 MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. Kennio Ferreira-Paim Leonardo Andrade-Silva Fernanda M Fonseca Thatiana B Ferreira Delio J Mora Juliana Andrade-Silva Aziza Khan Aiken Dao Eduardo C Reis Margarete T G Almeida Andre Maltos Virmondes R Junior Luciana Trilles Volker Rickerts Ariya Chindamporn Jane E Sykes Massimo Cogliati Kirsten Nielsen Teun Boekhout Matthew Fisher June Kwon-Chung David M Engelthaler Marcia Lazéra Wieland Meyer Mario L Silva-Vergara 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005223 https://doaj.org/article/26e9412f0b634d0b8c08d7fa8841f6d2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5242430?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005223 https://doaj.org/article/26e9412f0b634d0b8c08d7fa8841f6d2 PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005223 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005223 2022-12-31T02:44:24Z Cryptococcosis is an important fungal infection in immunocompromised individuals, especially those infected with HIV. In Brazil, despite the free availability of antiretroviral therapy (ART) in the public health system, the mortality rate due to Cryptococcus neoformans meningitis is still high. To obtain a more detailed picture of the population genetic structure of this species in southeast Brazil, we studied 108 clinical isolates from 101 patients and 35 environmental isolates. Among the patients, 59% had a fatal outcome mainly in HIV-positive male patients. All the isolates were found to be C. neoformans var. grubii major molecular type VNI and mating type locus alpha. Twelve were identified as diploid by flow cytometry, being homozygous (AαAα) for the mating type and by PCR screening of the STE20, GPA1, and PAK1 genes. Using the ISHAM consensus multilocus sequence typing (MLST) scheme, 13 sequence types (ST) were identified, with one being newly described. ST93 was identified from 81 (75%) of the clinical isolates, while ST77 and ST93 were identified from 19 (54%) and 10 (29%) environmental isolates, respectively. The southeastern Brazilian isolates had an overwhelming clonal population structure. When compared with populations from different continents based on data extracted from the ISHAM-MLST database (mlst.mycologylab.org) they showed less genetic variability. Two main clusters within C. neoformans var. grubii VNI were identified that diverged from VNB around 0.58 to 4.8 million years ago. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 1 e0005223 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kennio Ferreira-Paim Leonardo Andrade-Silva Fernanda M Fonseca Thatiana B Ferreira Delio J Mora Juliana Andrade-Silva Aziza Khan Aiken Dao Eduardo C Reis Margarete T G Almeida Andre Maltos Virmondes R Junior Luciana Trilles Volker Rickerts Ariya Chindamporn Jane E Sykes Massimo Cogliati Kirsten Nielsen Teun Boekhout Matthew Fisher June Kwon-Chung David M Engelthaler Marcia Lazéra Wieland Meyer Mario L Silva-Vergara MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cryptococcosis is an important fungal infection in immunocompromised individuals, especially those infected with HIV. In Brazil, despite the free availability of antiretroviral therapy (ART) in the public health system, the mortality rate due to Cryptococcus neoformans meningitis is still high. To obtain a more detailed picture of the population genetic structure of this species in southeast Brazil, we studied 108 clinical isolates from 101 patients and 35 environmental isolates. Among the patients, 59% had a fatal outcome mainly in HIV-positive male patients. All the isolates were found to be C. neoformans var. grubii major molecular type VNI and mating type locus alpha. Twelve were identified as diploid by flow cytometry, being homozygous (AαAα) for the mating type and by PCR screening of the STE20, GPA1, and PAK1 genes. Using the ISHAM consensus multilocus sequence typing (MLST) scheme, 13 sequence types (ST) were identified, with one being newly described. ST93 was identified from 81 (75%) of the clinical isolates, while ST77 and ST93 were identified from 19 (54%) and 10 (29%) environmental isolates, respectively. The southeastern Brazilian isolates had an overwhelming clonal population structure. When compared with populations from different continents based on data extracted from the ISHAM-MLST database (mlst.mycologylab.org) they showed less genetic variability. Two main clusters within C. neoformans var. grubii VNI were identified that diverged from VNB around 0.58 to 4.8 million years ago. |
format |
Article in Journal/Newspaper |
author |
Kennio Ferreira-Paim Leonardo Andrade-Silva Fernanda M Fonseca Thatiana B Ferreira Delio J Mora Juliana Andrade-Silva Aziza Khan Aiken Dao Eduardo C Reis Margarete T G Almeida Andre Maltos Virmondes R Junior Luciana Trilles Volker Rickerts Ariya Chindamporn Jane E Sykes Massimo Cogliati Kirsten Nielsen Teun Boekhout Matthew Fisher June Kwon-Chung David M Engelthaler Marcia Lazéra Wieland Meyer Mario L Silva-Vergara |
author_facet |
Kennio Ferreira-Paim Leonardo Andrade-Silva Fernanda M Fonseca Thatiana B Ferreira Delio J Mora Juliana Andrade-Silva Aziza Khan Aiken Dao Eduardo C Reis Margarete T G Almeida Andre Maltos Virmondes R Junior Luciana Trilles Volker Rickerts Ariya Chindamporn Jane E Sykes Massimo Cogliati Kirsten Nielsen Teun Boekhout Matthew Fisher June Kwon-Chung David M Engelthaler Marcia Lazéra Wieland Meyer Mario L Silva-Vergara |
author_sort |
Kennio Ferreira-Paim |
title |
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
title_short |
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
title_full |
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
title_fullStr |
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
title_full_unstemmed |
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil. |
title_sort |
mlst-based population genetic analysis in a global context reveals clonality amongst cryptococcus neoformans var. grubii vni isolates from hiv patients in southeastern brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005223 https://doaj.org/article/26e9412f0b634d0b8c08d7fa8841f6d2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005223 (2017) |
op_relation |
http://europepmc.org/articles/PMC5242430?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005223 https://doaj.org/article/26e9412f0b634d0b8c08d7fa8841f6d2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005223 |
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PLOS Neglected Tropical Diseases |
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11 |
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1 |
container_start_page |
e0005223 |
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