Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo
Abstract Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test...
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ftdoajarticles:oai:doaj.org/article:26d672a8c3d648018d12b6e373e36b58 2023-05-15T15:08:53+02:00 Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo Yawo Apetogbo Koffi M. Ahadji-Dabla Dieudonné Diloma Soma Adjovi D. Amoudji Edoh Koffi Kossivi I. Akagankou Rabila Bamogo Kelly Lionelle Ngaffo Samina Maiga Rachid T. Atcha‑Oubou Ameyo M. Dorkenoo Lucrecia Vizcaino Audrey Lenhart Abdoulaye Diabaté Roch Kounbobr Dabiré Guillaume Koffivi Ketoh 2022-11-01T00:00:00Z https://doi.org/10.1186/s12936-022-04377-2 https://doaj.org/article/26d672a8c3d648018d12b6e373e36b58 EN eng BMC https://doi.org/10.1186/s12936-022-04377-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04377-2 1475-2875 https://doaj.org/article/26d672a8c3d648018d12b6e373e36b58 Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) Malaria Anopheles gambiae Vector control Intensity resistance Togo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04377-2 2022-12-30T19:40:31Z Abstract Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test protocol. Pyrethroid resistance intensity bioassays were performed following the CDC bottle test protocol. The activity of detoxification enzymes was tested using the synergists piperonyl butoxide, S.S.S-tributlyphosphorotrithioate and ethacrinic acid. Species-specific identification of An. gambiae s.l. and kdr mutation genotyping were performed using PCR techniques. Results Local populations of An. gambiae s.l. showed full susceptibility to pirimiphos methyl at Lomé, Kovié, Anié, and Kpèlè Toutou. At Baguida, mortality was 90%, indicating possible resistance to pirimiphos methyl. Resistance was recorded to DDT, bendiocarb, and propoxur at all sites. A high intensity of pyrethroid resistance was recorded and the detoxification enzymes contributing to resistance were oxidases, esterases, and glutathione-s-transferases based on the synergist tests. Anopheles gambiae sensu stricto (s.s.) and Anopheles coluzzii were the main species identified. High kdr L1014F and low kdr L1014S allele frequencies were detected at all localities. Conclusion This study suggests the need to reinforce current insecticide-based malaria control interventions (IRS and LLINs) with complementary tools. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Anopheles gambiae Vector control Intensity resistance Togo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Anopheles gambiae Vector control Intensity resistance Togo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yawo Apetogbo Koffi M. Ahadji-Dabla Dieudonné Diloma Soma Adjovi D. Amoudji Edoh Koffi Kossivi I. Akagankou Rabila Bamogo Kelly Lionelle Ngaffo Samina Maiga Rachid T. Atcha‑Oubou Ameyo M. Dorkenoo Lucrecia Vizcaino Audrey Lenhart Abdoulaye Diabaté Roch Kounbobr Dabiré Guillaume Koffivi Ketoh Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
topic_facet |
Malaria Anopheles gambiae Vector control Intensity resistance Togo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test protocol. Pyrethroid resistance intensity bioassays were performed following the CDC bottle test protocol. The activity of detoxification enzymes was tested using the synergists piperonyl butoxide, S.S.S-tributlyphosphorotrithioate and ethacrinic acid. Species-specific identification of An. gambiae s.l. and kdr mutation genotyping were performed using PCR techniques. Results Local populations of An. gambiae s.l. showed full susceptibility to pirimiphos methyl at Lomé, Kovié, Anié, and Kpèlè Toutou. At Baguida, mortality was 90%, indicating possible resistance to pirimiphos methyl. Resistance was recorded to DDT, bendiocarb, and propoxur at all sites. A high intensity of pyrethroid resistance was recorded and the detoxification enzymes contributing to resistance were oxidases, esterases, and glutathione-s-transferases based on the synergist tests. Anopheles gambiae sensu stricto (s.s.) and Anopheles coluzzii were the main species identified. High kdr L1014F and low kdr L1014S allele frequencies were detected at all localities. Conclusion This study suggests the need to reinforce current insecticide-based malaria control interventions (IRS and LLINs) with complementary tools. |
format |
Article in Journal/Newspaper |
author |
Yawo Apetogbo Koffi M. Ahadji-Dabla Dieudonné Diloma Soma Adjovi D. Amoudji Edoh Koffi Kossivi I. Akagankou Rabila Bamogo Kelly Lionelle Ngaffo Samina Maiga Rachid T. Atcha‑Oubou Ameyo M. Dorkenoo Lucrecia Vizcaino Audrey Lenhart Abdoulaye Diabaté Roch Kounbobr Dabiré Guillaume Koffivi Ketoh |
author_facet |
Yawo Apetogbo Koffi M. Ahadji-Dabla Dieudonné Diloma Soma Adjovi D. Amoudji Edoh Koffi Kossivi I. Akagankou Rabila Bamogo Kelly Lionelle Ngaffo Samina Maiga Rachid T. Atcha‑Oubou Ameyo M. Dorkenoo Lucrecia Vizcaino Audrey Lenhart Abdoulaye Diabaté Roch Kounbobr Dabiré Guillaume Koffivi Ketoh |
author_sort |
Yawo Apetogbo |
title |
Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
title_short |
Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
title_full |
Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
title_fullStr |
Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
title_full_unstemmed |
Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo |
title_sort |
insecticide resistance intensity and efficacy of synergists with pyrethroids in anopheles gambiae (diptera: culicidae) from southern togo |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s12936-022-04377-2 https://doaj.org/article/26d672a8c3d648018d12b6e373e36b58 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 21, Iss 1, Pp 1-10 (2022) |
op_relation |
https://doi.org/10.1186/s12936-022-04377-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04377-2 1475-2875 https://doaj.org/article/26d672a8c3d648018d12b6e373e36b58 |
op_doi |
https://doi.org/10.1186/s12936-022-04377-2 |
container_title |
Malaria Journal |
container_volume |
21 |
container_issue |
1 |
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1766340152793235456 |