Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens
Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal distr...
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ftdoajarticles:oai:doaj.org/article:26b734480182451a8e998f139dd2e024 2024-09-09T19:25:09+00:00 Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens BRAGAZZA Lúcia M. VAZ Adelaide J. PASSOS Afonso D.C. TAKAYANAGUI Osvaldo M. NAKAMURA Paulo M. ESPÍNDOLA Noeli M. PARDINI Alessandra BUENO Ednéia C. 2002-01-01T00:00:00Z https://doaj.org/article/26b734480182451a8e998f139dd2e024 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000100002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/26b734480182451a8e998f139dd2e024 Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 1, Pp 7-12 (2002) Cysticercosis Seroprevalence Immunologic tests Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2002 ftdoajarticles 2024-08-05T17:49:30Z Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Cysticercosis Seroprevalence Immunologic tests Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Cysticercosis Seroprevalence Immunologic tests Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 BRAGAZZA Lúcia M. VAZ Adelaide J. PASSOS Afonso D.C. TAKAYANAGUI Osvaldo M. NAKAMURA Paulo M. ESPÍNDOLA Noeli M. PARDINI Alessandra BUENO Ednéia C. Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
topic_facet |
Cysticercosis Seroprevalence Immunologic tests Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%. |
format |
Article in Journal/Newspaper |
author |
BRAGAZZA Lúcia M. VAZ Adelaide J. PASSOS Afonso D.C. TAKAYANAGUI Osvaldo M. NAKAMURA Paulo M. ESPÍNDOLA Noeli M. PARDINI Alessandra BUENO Ednéia C. |
author_facet |
BRAGAZZA Lúcia M. VAZ Adelaide J. PASSOS Afonso D.C. TAKAYANAGUI Osvaldo M. NAKAMURA Paulo M. ESPÍNDOLA Noeli M. PARDINI Alessandra BUENO Ednéia C. |
author_sort |
BRAGAZZA Lúcia M. |
title |
Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
title_short |
Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
title_full |
Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
title_fullStr |
Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
title_full_unstemmed |
Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens |
title_sort |
frequency of serum anti-cysticercus antibodies in the population of a rural brazilian community (cássia dos coqueiros, sp) determined by elisa and immunoblotting using taenia crassiceps antigens |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2002 |
url |
https://doaj.org/article/26b734480182451a8e998f139dd2e024 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 1, Pp 7-12 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000100002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/26b734480182451a8e998f139dd2e024 |
_version_ |
1809894924309495808 |