Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium
Abstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various ind...
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ftdoajarticles:oai:doaj.org/article:26aad25c69774e0f9e1d4977f5a42c81 2023-05-15T13:53:19+02:00 Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium P. Rios B. Bezus S. Cavalitto I. Cavello 2022-05-01T00:00:00Z https://doi.org/10.1186/s43141-022-00356-x https://doaj.org/article/26aad25c69774e0f9e1d4977f5a42c81 EN eng SpringerOpen https://doi.org/10.1186/s43141-022-00356-x https://doaj.org/toc/2090-5920 doi:10.1186/s43141-022-00356-x 2090-5920 https://doaj.org/article/26aad25c69774e0f9e1d4977f5a42c81 Journal of Genetic Engineering and Biotechnology, Vol 20, Iss 1, Pp 1-14 (2022) Cold active keratinase Pedobacter Keratin biodegradation Biotechnology TP248.13-248.65 Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.1186/s43141-022-00356-x 2022-12-31T02:34:35Z Abstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h−1). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml−1) and keratinolytic (33.04 ± 1.09 U ml−1) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Journal of Genetic Engineering and Biotechnology 20 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cold active keratinase Pedobacter Keratin biodegradation Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Cold active keratinase Pedobacter Keratin biodegradation Biotechnology TP248.13-248.65 Genetics QH426-470 P. Rios B. Bezus S. Cavalitto I. Cavello Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
topic_facet |
Cold active keratinase Pedobacter Keratin biodegradation Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background Antarctica is one of the harshest environments in the world. Despite this fact, it has been colonized by microorganisms, which had to develop different adaptations in order to survive. By studying their enzymes, we can harness these adaptations in order to use them in various industrial processes. Keratinases (E.C. 3.4.99.11) are characterized by their robustness in withstanding extreme conditions and, along with other enzymes, are commonly added to laundry detergents, which makes their study of industrial interest. Results In this work, a novel keratinase producer, Pedobacter sp. 3.14.7 (MF 347939.1), isolated from Antarctic birds’ nests, was identified. This psychrotolerant isolate displays a typical psychrotolerant growth pattern, with an optimal temperature of 20 °C (μmax=0.23 h−1). After 238 h, maximum proteolytic (22.00 ± 1.17 U ml−1) and keratinolytic (33.04 ± 1.09 U ml−1) activities were achieved with a feather sample conversion of approximately 85%. The keratinase present in crude extract was characterized as a metalloprotease with a molecular weight of 25 kDa, stable in a wide range of pH, with an optimum pH of 7.5. Optimum temperature was 55 °C. Wash performance at 20 °C using this crude extract could remove completely blood stain from cotton cloth. Conclusion We report a new keratinolytic bacteria from maritime Antarctica. Among its biochemical characteristics, its stability in the presence of different detergents and bleaching agents and its wash performance showed promising results regarding its potential use as a laundry detergent additive. |
format |
Article in Journal/Newspaper |
author |
P. Rios B. Bezus S. Cavalitto I. Cavello |
author_facet |
P. Rios B. Bezus S. Cavalitto I. Cavello |
author_sort |
P. Rios |
title |
Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
title_short |
Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
title_full |
Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
title_fullStr |
Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
title_full_unstemmed |
Production and characterization of a new detergent-stable keratinase expressed by Pedobacter sp. 3.14.7, a novel Antarctic psychrotolerant keratin-degrading bacterium |
title_sort |
production and characterization of a new detergent-stable keratinase expressed by pedobacter sp. 3.14.7, a novel antarctic psychrotolerant keratin-degrading bacterium |
publisher |
SpringerOpen |
publishDate |
2022 |
url |
https://doi.org/10.1186/s43141-022-00356-x https://doaj.org/article/26aad25c69774e0f9e1d4977f5a42c81 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal of Genetic Engineering and Biotechnology, Vol 20, Iss 1, Pp 1-14 (2022) |
op_relation |
https://doi.org/10.1186/s43141-022-00356-x https://doaj.org/toc/2090-5920 doi:10.1186/s43141-022-00356-x 2090-5920 https://doaj.org/article/26aad25c69774e0f9e1d4977f5a42c81 |
op_doi |
https://doi.org/10.1186/s43141-022-00356-x |
container_title |
Journal of Genetic Engineering and Biotechnology |
container_volume |
20 |
container_issue |
1 |
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1766258364995600384 |