Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis.
The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside...
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ftdoajarticles:oai:doaj.org/article:269e9892cf8a49e18f34188c47a2544a 2023-05-15T15:12:08+02:00 Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. Thais Bonifácio Campolina Luis Eduardo Martinez Villegas Carolina Cunha Monteiro Paulo Filemon Paolucci Pimenta Nagila Francinete Costa Secundino 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008666 https://doaj.org/article/269e9892cf8a49e18f34188c47a2544a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008666 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008666 https://doaj.org/article/269e9892cf8a49e18f34188c47a2544a PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008666 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008666 2022-12-31T10:59:43Z The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Providencia ENVELOPE(-66.779,-66.779,-68.305,-68.305) PLOS Neglected Tropical Diseases 14 10 e0008666 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Thais Bonifácio Campolina Luis Eduardo Martinez Villegas Carolina Cunha Monteiro Paulo Filemon Paolucci Pimenta Nagila Francinete Costa Secundino Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania. |
format |
Article in Journal/Newspaper |
author |
Thais Bonifácio Campolina Luis Eduardo Martinez Villegas Carolina Cunha Monteiro Paulo Filemon Paolucci Pimenta Nagila Francinete Costa Secundino |
author_facet |
Thais Bonifácio Campolina Luis Eduardo Martinez Villegas Carolina Cunha Monteiro Paulo Filemon Paolucci Pimenta Nagila Francinete Costa Secundino |
author_sort |
Thais Bonifácio Campolina |
title |
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
title_short |
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
title_full |
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
title_fullStr |
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
title_full_unstemmed |
Tripartite interactions: Leishmania, microbiota and Lutzomyia longipalpis. |
title_sort |
tripartite interactions: leishmania, microbiota and lutzomyia longipalpis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008666 https://doaj.org/article/269e9892cf8a49e18f34188c47a2544a |
long_lat |
ENVELOPE(-66.779,-66.779,-68.305,-68.305) |
geographic |
Arctic Providencia |
geographic_facet |
Arctic Providencia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008666 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008666 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008666 https://doaj.org/article/269e9892cf8a49e18f34188c47a2544a |
op_doi |
https://doi.org/10.1371/journal.pntd.0008666 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0008666 |
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1766342855124582400 |