A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar )
The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respect...
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ftdoajarticles:oai:doaj.org/article:269a05c6ee064fa397bfb65aa7c04ace 2023-05-15T15:30:46+02:00 A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) Bjørg Kristine Hundal Esmail Lutfi Trygve Sigholt Grethe Rosenlund Nina Sylvia Liland Brett Glencross Nini Hedberg Sissener 2022-02-01T00:00:00Z https://doi.org/10.3390/metabo12020159 https://doaj.org/article/269a05c6ee064fa397bfb65aa7c04ace EN eng MDPI AG https://www.mdpi.com/2218-1989/12/2/159 https://doaj.org/toc/2218-1989 doi:10.3390/metabo12020159 2218-1989 https://doaj.org/article/269a05c6ee064fa397bfb65aa7c04ace Metabolites, Vol 12, Iss 159, p 159 (2022) lipid metabolism EPA DHA robustness Atlantic salmon metabolomics Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.3390/metabo12020159 2022-12-31T15:59:36Z The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respectively) were fed in triplicate through a full production cycle from an average starting weight of 275 g to slaughter size (~5 kg). Feeding low dietary EPA + DHA altered the hepatic energy metabolism, evidenced by reductions in tricarboxylic acid cycle intermediates originating from β-oxidation, which was compensated by elevated activity in alternative energy pathways (pentose phosphate pathway, branched chain amino acid catabolism and creatine metabolism). Increases in various acylcarnitines in the liver supported this and indicates issues with lipid metabolism (mitochondrial β-oxidation). Problems using lipids for energy in the lower EPA + DHA groups line up well with observed increases in liver lipids in these fish. It also aligns with the growth data, where fish fed the highest EPA + DHA grew better than the other groups. The study showed that diets 1.0 and 1.3 were insufficient for maintaining good liver metabolic health. However, diet 3.5 was significantly better than diet 1.6, indicating that diet 1.6 might also be suboptimal. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Metabolites 12 2 159 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
lipid metabolism EPA DHA robustness Atlantic salmon metabolomics Microbiology QR1-502 |
spellingShingle |
lipid metabolism EPA DHA robustness Atlantic salmon metabolomics Microbiology QR1-502 Bjørg Kristine Hundal Esmail Lutfi Trygve Sigholt Grethe Rosenlund Nina Sylvia Liland Brett Glencross Nini Hedberg Sissener A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
topic_facet |
lipid metabolism EPA DHA robustness Atlantic salmon metabolomics Microbiology QR1-502 |
description |
The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respectively) were fed in triplicate through a full production cycle from an average starting weight of 275 g to slaughter size (~5 kg). Feeding low dietary EPA + DHA altered the hepatic energy metabolism, evidenced by reductions in tricarboxylic acid cycle intermediates originating from β-oxidation, which was compensated by elevated activity in alternative energy pathways (pentose phosphate pathway, branched chain amino acid catabolism and creatine metabolism). Increases in various acylcarnitines in the liver supported this and indicates issues with lipid metabolism (mitochondrial β-oxidation). Problems using lipids for energy in the lower EPA + DHA groups line up well with observed increases in liver lipids in these fish. It also aligns with the growth data, where fish fed the highest EPA + DHA grew better than the other groups. The study showed that diets 1.0 and 1.3 were insufficient for maintaining good liver metabolic health. However, diet 3.5 was significantly better than diet 1.6, indicating that diet 1.6 might also be suboptimal. |
format |
Article in Journal/Newspaper |
author |
Bjørg Kristine Hundal Esmail Lutfi Trygve Sigholt Grethe Rosenlund Nina Sylvia Liland Brett Glencross Nini Hedberg Sissener |
author_facet |
Bjørg Kristine Hundal Esmail Lutfi Trygve Sigholt Grethe Rosenlund Nina Sylvia Liland Brett Glencross Nini Hedberg Sissener |
author_sort |
Bjørg Kristine Hundal |
title |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
title_short |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
title_full |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
title_fullStr |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
title_full_unstemmed |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon ( Salmo salar ) |
title_sort |
piece of the puzzle—possible mechanisms for why low dietary epa and dha cause hepatic lipid accumulation in atlantic salmon ( salmo salar ) |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/metabo12020159 https://doaj.org/article/269a05c6ee064fa397bfb65aa7c04ace |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Slaughter |
geographic_facet |
Slaughter |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Metabolites, Vol 12, Iss 159, p 159 (2022) |
op_relation |
https://www.mdpi.com/2218-1989/12/2/159 https://doaj.org/toc/2218-1989 doi:10.3390/metabo12020159 2218-1989 https://doaj.org/article/269a05c6ee064fa397bfb65aa7c04ace |
op_doi |
https://doi.org/10.3390/metabo12020159 |
container_title |
Metabolites |
container_volume |
12 |
container_issue |
2 |
container_start_page |
159 |
_version_ |
1766361227181686784 |