The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics
BackgroundInvasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to...
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ftdoajarticles:oai:doaj.org/article:2680203bf6b74b6db9ef341be67528d2 2023-05-15T16:51:01+02:00 The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo 2021-02-01T00:00:00Z https://doi.org/10.3389/fmicb.2021.637813 https://doaj.org/article/2680203bf6b74b6db9ef341be67528d2 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmicb.2021.637813/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2021.637813 https://doaj.org/article/2680203bf6b74b6db9ef341be67528d2 Frontiers in Microbiology, Vol 12 (2021) Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3389/fmicb.2021.637813 2022-12-31T13:44:43Z BackgroundInvasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to a non-PCV serotype, were detected through the Lebanese Inter-Hospital Pneumococcal Surveillance Program. The aim of the study is to characterize phenotypic and genomic features of the 24F isolates in Lebanon.MethodsWGS using long reads sequencing (PacBio) was performed to produce complete circular genomes and to determine clonality, antimicrobial resistance and virulence determinants.ResultsThe sequencing results yielded eight closed circular genomes. Three multilocus sequence typing (MLST) types were identified (ST11618, ST14184, ST15253). Both MLST and WGS analyses revealed that these isolates from Lebanon were genetically homogenous belonging to clonal complex CC230 and clustered closely with isolates originating from Canada, United States of America, United Kingdom and Iceland. Their penicillin binding protein profiles correlated with both β-lactam susceptibility patterns and MLST types. Moreover, the isolates harbored the macrolide and tetracycline resistance genes and showed a similar virulence gene profile. To our knowledge, this study represents the first report of complete phenotypic and genomic characterization of the emerging Streptococcus pneumoniae, serotype 24F, in the Middle East and North Africa region. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Canada Frontiers in Microbiology 12 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Microbiology QR1-502 |
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Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Microbiology QR1-502 Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
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Streptococcus pneumoniae serotype 24F whole-genome sequencing Lebanon antibiotic resistance Microbiology QR1-502 |
description |
BackgroundInvasive pneumococcal disease (IPD) remains a global health problem. IPD incidence has significantly decreased by the use of pneumococcal conjugate vaccines (PCV). Nevertheless, non-PCV serotypes remain a matter of concern. Eight Streptococcus pneumoniae serotype 24F isolates, belonging to a non-PCV serotype, were detected through the Lebanese Inter-Hospital Pneumococcal Surveillance Program. The aim of the study is to characterize phenotypic and genomic features of the 24F isolates in Lebanon.MethodsWGS using long reads sequencing (PacBio) was performed to produce complete circular genomes and to determine clonality, antimicrobial resistance and virulence determinants.ResultsThe sequencing results yielded eight closed circular genomes. Three multilocus sequence typing (MLST) types were identified (ST11618, ST14184, ST15253). Both MLST and WGS analyses revealed that these isolates from Lebanon were genetically homogenous belonging to clonal complex CC230 and clustered closely with isolates originating from Canada, United States of America, United Kingdom and Iceland. Their penicillin binding protein profiles correlated with both β-lactam susceptibility patterns and MLST types. Moreover, the isolates harbored the macrolide and tetracycline resistance genes and showed a similar virulence gene profile. To our knowledge, this study represents the first report of complete phenotypic and genomic characterization of the emerging Streptococcus pneumoniae, serotype 24F, in the Middle East and North Africa region. |
format |
Article in Journal/Newspaper |
author |
Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo |
author_facet |
Lina Reslan Marc Finianos Ibrahim Bitar Mohamad Bahij Moumneh George F. Araj Alissar Zaghlout Celina Boutros Tamima Jisr Malak Nabulsi Gilbert Kara yaccoub Monzer Hamze Marwan Osman Elie Bou Raad Jaroslav Hrabak Ghassan M. Matar Ghassan Dbaibo |
author_sort |
Lina Reslan |
title |
The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
title_short |
The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
title_full |
The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
title_fullStr |
The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
title_full_unstemmed |
The Emergence of Invasive Streptococcus pneumoniae Serotype 24F in Lebanon: Complete Genome Sequencing Reveals High Virulence and Antimicrobial Resistance Characteristics |
title_sort |
emergence of invasive streptococcus pneumoniae serotype 24f in lebanon: complete genome sequencing reveals high virulence and antimicrobial resistance characteristics |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmicb.2021.637813 https://doaj.org/article/2680203bf6b74b6db9ef341be67528d2 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Frontiers in Microbiology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.637813/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2021.637813 https://doaj.org/article/2680203bf6b74b6db9ef341be67528d2 |
op_doi |
https://doi.org/10.3389/fmicb.2021.637813 |
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Frontiers in Microbiology |
container_volume |
12 |
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1766041134127120384 |