Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells
Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:25ce092ae5c34a82be564bb405ac6908 2023-05-15T15:06:31+02:00 Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin 2015-05-01T00:00:00Z https://doi.org/10.1016/S2221-1691(15)30372-5 https://doaj.org/article/25ce092ae5c34a82be564bb405ac6908 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115303725 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30372-5 https://doaj.org/article/25ce092ae5c34a82be564bb405ac6908 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 5, Pp 375-381 (2015) HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/S2221-1691(15)30372-5 2022-12-31T15:24:07Z Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results: The IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 5 375 381 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
topic_facet |
HepG2 Hepatocellular carcinoma Aloe vera Calligonum comosum Apoptosis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. Methods: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. Results: The IC50 values of A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) µg/mL respectively. The extracts separately increased cytotoxicity against HepG2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions. Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis. |
format |
Article in Journal/Newspaper |
author |
Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin |
author_facet |
Maram Shalabi Kh. Khilo Mahmoud M. Zakaria Mahmoud G. Elsebaei Walied Abdo Walaa Awadin |
author_sort |
Maram Shalabi |
title |
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
title_short |
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
title_full |
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
title_fullStr |
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
title_full_unstemmed |
Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells |
title_sort |
anticancer activity of aloe vera and calligonum comosum extracts separetely on hepatocellular carcinoma cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/S2221-1691(15)30372-5 https://doaj.org/article/25ce092ae5c34a82be564bb405ac6908 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 5, Pp 375-381 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115303725 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30372-5 https://doaj.org/article/25ce092ae5c34a82be564bb405ac6908 |
op_doi |
https://doi.org/10.1016/S2221-1691(15)30372-5 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
5 |
container_start_page |
375 |
op_container_end_page |
381 |
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1766338101422063616 |