Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates.
Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0002140 https://doaj.org/article/25a6fcaff31944438fab70be6dda0781 |
id |
ftdoajarticles:oai:doaj.org/article:25a6fcaff31944438fab70be6dda0781 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:25a6fcaff31944438fab70be6dda0781 2023-05-15T15:10:00+02:00 Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002140 https://doaj.org/article/25a6fcaff31944438fab70be6dda0781 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23556024/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002140 https://doaj.org/article/25a6fcaff31944438fab70be6dda0781 PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2140 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002140 2022-12-31T05:47:44Z Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 3 e2140 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans. |
format |
Article in Journal/Newspaper |
author |
Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý |
author_facet |
Kateřina Pomajbíková Miroslav Oborník Aleš Horák Klára J Petrželková J Norman Grim Bruno Levecke Angelique Todd Martin Mulama John Kiyang David Modrý |
author_sort |
Kateřina Pomajbíková |
title |
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
title_short |
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
title_full |
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
title_fullStr |
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
title_full_unstemmed |
Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates. |
title_sort |
novel insights into the genetic diversity of balantidium and balantidium-like cyst-forming ciliates. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002140 https://doaj.org/article/25a6fcaff31944438fab70be6dda0781 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2140 (2013) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23556024/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002140 https://doaj.org/article/25a6fcaff31944438fab70be6dda0781 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002140 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
3 |
container_start_page |
e2140 |
_version_ |
1766341083151728640 |