Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection
Abstract Background In the absence of a method to culture Plasmodium vivax, the only way to source parasites is ex vivo. This hampers many aspects of P. vivax research. This study aimed to assess the safety of apheresis, a method for selective removal of specific components of blood as a means of ex...
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ftdoajarticles:oai:doaj.org/article:253ef020fa54471ea40773e4a1b11bb7 2023-05-15T15:15:34+02:00 Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection Anand Odedra Kari Mudie Glen Kennedy Rebecca E. Watts Emilie Rossignol Hayley Mitchell Jeremy Gower Maria Rebelo Zuleima Pava Rebecca Pawliw Stephen Woolley David G. Lalloo Greg Robinson Sean Lynch Katharine A. Collins Fiona Amante James McCarthy 2021-01-01T00:00:00Z https://doi.org/10.1186/s12936-021-03581-w https://doaj.org/article/253ef020fa54471ea40773e4a1b11bb7 EN eng BMC https://doi.org/10.1186/s12936-021-03581-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03581-w 1475-2875 https://doaj.org/article/253ef020fa54471ea40773e4a1b11bb7 Malaria Journal, Vol 20, Iss 1, Pp 1-14 (2021) Malaria Plasmodium Apheresis Parasite Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03581-w 2022-12-31T07:41:22Z Abstract Background In the absence of a method to culture Plasmodium vivax, the only way to source parasites is ex vivo. This hampers many aspects of P. vivax research. This study aimed to assess the safety of apheresis, a method for selective removal of specific components of blood as a means of extracting and concentrating P. vivax parasites. Methods An iterative approach was employed across four non-immune healthy human subjects in single subject cohorts. All four subjects were inoculated with ~ 564 blood stage P. vivax (HMP013-Pv) and subjected to apheresis 10 to 11 days later. Blood samples collected during apheresis (haematocrit layers 0.5% to 11%) were tested for the presence and concentration of P. vivax by microscopy, flow cytometry, 18S rDNA qPCR for total parasites, and pvs25 qRT-PCR for female gametocyte transcripts. Safety was determined by monitoring adverse events. Malaria transmission to mosquitoes was assessed by membrane feeding assays. Results There were no serious adverse events and no significant safety concerns. Apheresis concentrated asexual parasites by up to 4.9-fold (range: 0.9–4.9-fold) and gametocytes by up to 1.45-fold (range: 0.38–1.45-fold) compared to pre-apheresis densities. No single haematocrit layer contained > 40% of all the recovered P. vivax asexual parasites. Ex vivo concentration of parasites by Percoll gradient centrifugation of whole blood achieved greater concentration of gametocytes than apheresis. Mosquito transmission was enhanced by up to fivefold in a single apheresis sample compared to pre-apheresis. Conclusion The modest level of parasite concentration suggests that the use of apheresis may not be an ideal method for harvesting P. vivax. Trial Registration Australia New Zealand Clinical Trials Registry (ANZCTR) Trial ID: ACTRN12617001502325 registered on 19th October 2017. https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=373812. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic New Zealand Malaria Journal 20 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Plasmodium Apheresis Parasite Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Plasmodium Apheresis Parasite Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Anand Odedra Kari Mudie Glen Kennedy Rebecca E. Watts Emilie Rossignol Hayley Mitchell Jeremy Gower Maria Rebelo Zuleima Pava Rebecca Pawliw Stephen Woolley David G. Lalloo Greg Robinson Sean Lynch Katharine A. Collins Fiona Amante James McCarthy Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
topic_facet |
Malaria Plasmodium Apheresis Parasite Concentration Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In the absence of a method to culture Plasmodium vivax, the only way to source parasites is ex vivo. This hampers many aspects of P. vivax research. This study aimed to assess the safety of apheresis, a method for selective removal of specific components of blood as a means of extracting and concentrating P. vivax parasites. Methods An iterative approach was employed across four non-immune healthy human subjects in single subject cohorts. All four subjects were inoculated with ~ 564 blood stage P. vivax (HMP013-Pv) and subjected to apheresis 10 to 11 days later. Blood samples collected during apheresis (haematocrit layers 0.5% to 11%) were tested for the presence and concentration of P. vivax by microscopy, flow cytometry, 18S rDNA qPCR for total parasites, and pvs25 qRT-PCR for female gametocyte transcripts. Safety was determined by monitoring adverse events. Malaria transmission to mosquitoes was assessed by membrane feeding assays. Results There were no serious adverse events and no significant safety concerns. Apheresis concentrated asexual parasites by up to 4.9-fold (range: 0.9–4.9-fold) and gametocytes by up to 1.45-fold (range: 0.38–1.45-fold) compared to pre-apheresis densities. No single haematocrit layer contained > 40% of all the recovered P. vivax asexual parasites. Ex vivo concentration of parasites by Percoll gradient centrifugation of whole blood achieved greater concentration of gametocytes than apheresis. Mosquito transmission was enhanced by up to fivefold in a single apheresis sample compared to pre-apheresis. Conclusion The modest level of parasite concentration suggests that the use of apheresis may not be an ideal method for harvesting P. vivax. Trial Registration Australia New Zealand Clinical Trials Registry (ANZCTR) Trial ID: ACTRN12617001502325 registered on 19th October 2017. https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=373812. |
format |
Article in Journal/Newspaper |
author |
Anand Odedra Kari Mudie Glen Kennedy Rebecca E. Watts Emilie Rossignol Hayley Mitchell Jeremy Gower Maria Rebelo Zuleima Pava Rebecca Pawliw Stephen Woolley David G. Lalloo Greg Robinson Sean Lynch Katharine A. Collins Fiona Amante James McCarthy |
author_facet |
Anand Odedra Kari Mudie Glen Kennedy Rebecca E. Watts Emilie Rossignol Hayley Mitchell Jeremy Gower Maria Rebelo Zuleima Pava Rebecca Pawliw Stephen Woolley David G. Lalloo Greg Robinson Sean Lynch Katharine A. Collins Fiona Amante James McCarthy |
author_sort |
Anand Odedra |
title |
Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
title_short |
Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
title_full |
Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
title_fullStr |
Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
title_full_unstemmed |
Safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage Plasmodium vivax infection |
title_sort |
safety and feasibility of apheresis to harvest and concentrate parasites from subjects with induced blood stage plasmodium vivax infection |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03581-w https://doaj.org/article/253ef020fa54471ea40773e4a1b11bb7 |
geographic |
Arctic New Zealand |
geographic_facet |
Arctic New Zealand |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-14 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03581-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03581-w 1475-2875 https://doaj.org/article/253ef020fa54471ea40773e4a1b11bb7 |
op_doi |
https://doi.org/10.1186/s12936-021-03581-w |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766345938043928576 |