Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis

• Published in: Genes
• Main Authors: Dominique J. Wiener, Kátia R. Groch, Magdalena A.T. Brunner, Tosso Leeb, Vidhya Jagannathan, Monika M. Welle
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2020
• Subjects: dog; Canis lupus familiaris; dermatology; hair cycle; microdissection; transcriptome analysis; Genetics; QH426-470; Canis lupus
• Online Access: https://doi.org/10.3390/genes11080884; https://doaj.org/article/25308531c4d041519cbff71388ffaf86

The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15 . The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states.