A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection.
Background Bone formation and loss are the characteristic clinical manifestations of leprosy, but the mechanisms underlying the bone remodeling with Mycobacterium leprae (M. leprae) infection are unclear. Methodology/principal findings Osteocytes may have a role through regulating the differentiatio...
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Online Access: | https://doi.org/10.1371/journal.pntd.0010379 https://doaj.org/article/2527e83760db4c3198b7e488e29d8f1d |
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ftdoajarticles:oai:doaj.org/article:2527e83760db4c3198b7e488e29d8f1d 2023-05-15T15:08:52+02:00 A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. Zheng-Rong Gao Qiong Liu Jie Zhao Ya-Qiong Zhao Li Tan Shao-Hui Zhang Ying-Hui Zhou Yun Chen Yue Guo Yun-Zhi Feng 2022-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010379 https://doaj.org/article/2527e83760db4c3198b7e488e29d8f1d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010379 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010379 https://doaj.org/article/2527e83760db4c3198b7e488e29d8f1d PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010379 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010379 2022-12-31T03:02:50Z Background Bone formation and loss are the characteristic clinical manifestations of leprosy, but the mechanisms underlying the bone remodeling with Mycobacterium leprae (M. leprae) infection are unclear. Methodology/principal findings Osteocytes may have a role through regulating the differentiation of osteogenic lineages. To investigate osteocyte-related mechanisms in leprosy, we treated osteocyte-like cell with N-glycosylated muramyl dipeptide (N.g MDP). RNA-seq analysis showed 724 differentially expressed messenger RNAs (mRNAs) and 724 differentially expressed circular RNA (circRNAs). Of these, we filtered through eight osteogenic-related differentially expressed genes, according to the characteristic of competing endogenous RNA, PubMed databases, and bioinformatic analysis, including TargetScan, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes. Based on these results, we built a circRNA-microRNA (miRNA)-mRNA triple network. Quantitative reverse-transcription polymerase chain reaction and western blots analyses confirmed decreased Clock expression in osteocyte-like cell, while increased in bone mesenchymal stem cells (BMSCs), implicating a crucial factor in osteogenic differentiation. Immunohistochemistry showed obviously increased expression of CLOCK protein in BMSCs and osteoblasts in N.g MDP-treated mice, but decreased expression in osteocytes. Conclusions/significance This analytical method provided a basis for the relationship between N.g MDP and remodeling in osteocytes, and the circRNA-miRNA-mRNA triple network may offer a new target for leprosy therapeutics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 5 e0010379 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zheng-Rong Gao Qiong Liu Jie Zhao Ya-Qiong Zhao Li Tan Shao-Hui Zhang Ying-Hui Zhou Yun Chen Yue Guo Yun-Zhi Feng A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Bone formation and loss are the characteristic clinical manifestations of leprosy, but the mechanisms underlying the bone remodeling with Mycobacterium leprae (M. leprae) infection are unclear. Methodology/principal findings Osteocytes may have a role through regulating the differentiation of osteogenic lineages. To investigate osteocyte-related mechanisms in leprosy, we treated osteocyte-like cell with N-glycosylated muramyl dipeptide (N.g MDP). RNA-seq analysis showed 724 differentially expressed messenger RNAs (mRNAs) and 724 differentially expressed circular RNA (circRNAs). Of these, we filtered through eight osteogenic-related differentially expressed genes, according to the characteristic of competing endogenous RNA, PubMed databases, and bioinformatic analysis, including TargetScan, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes. Based on these results, we built a circRNA-microRNA (miRNA)-mRNA triple network. Quantitative reverse-transcription polymerase chain reaction and western blots analyses confirmed decreased Clock expression in osteocyte-like cell, while increased in bone mesenchymal stem cells (BMSCs), implicating a crucial factor in osteogenic differentiation. Immunohistochemistry showed obviously increased expression of CLOCK protein in BMSCs and osteoblasts in N.g MDP-treated mice, but decreased expression in osteocytes. Conclusions/significance This analytical method provided a basis for the relationship between N.g MDP and remodeling in osteocytes, and the circRNA-miRNA-mRNA triple network may offer a new target for leprosy therapeutics. |
format |
Article in Journal/Newspaper |
author |
Zheng-Rong Gao Qiong Liu Jie Zhao Ya-Qiong Zhao Li Tan Shao-Hui Zhang Ying-Hui Zhou Yun Chen Yue Guo Yun-Zhi Feng |
author_facet |
Zheng-Rong Gao Qiong Liu Jie Zhao Ya-Qiong Zhao Li Tan Shao-Hui Zhang Ying-Hui Zhou Yun Chen Yue Guo Yun-Zhi Feng |
author_sort |
Zheng-Rong Gao |
title |
A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
title_short |
A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
title_full |
A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
title_fullStr |
A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
title_full_unstemmed |
A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. |
title_sort |
comprehensive analysis of the circrna-mirna-mrna network in osteocyte-like cell associated with mycobacterium leprae infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010379 https://doaj.org/article/2527e83760db4c3198b7e488e29d8f1d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010379 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010379 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010379 https://doaj.org/article/2527e83760db4c3198b7e488e29d8f1d |
op_doi |
https://doi.org/10.1371/journal.pntd.0010379 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
5 |
container_start_page |
e0010379 |
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1766340152617074688 |