Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.

PGE2 plays an important role in a broad spectrum of physiological and pathological processes mediated through a membrane-bound G protein-coupled receptor (GPCR) called EP receptor. In mammals, four subtypes of EP receptor (EP 1-4) are identified and each of them functions through different signal tr...

Full description

Bibliographic Details
Published in:PLOS ONE
Main Authors: Tz Chun Guo, Amr Ahmed Abdelrahim Gamil, Melanie Koenig, Øystein Evensen
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2015
Subjects:
R
Q
Online Access:https://doi.org/10.1371/journal.pone.0120483
https://doaj.org/article/251bcd2b4d9f492a960d0214c10c865e
id ftdoajarticles:oai:doaj.org/article:251bcd2b4d9f492a960d0214c10c865e
record_format openpolar
spelling ftdoajarticles:oai:doaj.org/article:251bcd2b4d9f492a960d0214c10c865e 2023-05-15T15:30:31+02:00 Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro. Tz Chun Guo Amr Ahmed Abdelrahim Gamil Melanie Koenig Øystein Evensen 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0120483 https://doaj.org/article/251bcd2b4d9f492a960d0214c10c865e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4383505?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0120483 https://doaj.org/article/251bcd2b4d9f492a960d0214c10c865e PLoS ONE, Vol 10, Iss 3, p e0120483 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0120483 2022-12-31T14:32:45Z PGE2 plays an important role in a broad spectrum of physiological and pathological processes mediated through a membrane-bound G protein-coupled receptor (GPCR) called EP receptor. In mammals, four subtypes of EP receptor (EP 1-4) are identified and each of them functions through different signal transduction pathways. Orthologous EP receptors have also been identified in other non-mammalian species, such as chicken and zebrafish. EP4 is the only identified PGE2 receptor to date in Atlantic salmon but its tissue distribution and function have not been studied in any detail. In this study, we first sequenced EP4 receptor in different tissues and found that the presence of the 3nt deletion in the 5' untranslated region was accompanied by silent mutation at nt 668. While attempting to amplify the same sequence in TO cells (an Atlantic salmon macrophage-like cell line), we failed to obtain the full-length product. Further investigation revealed different isoform of EP4 receptor in TO cells and we subsequently documented its presence in different Atlantic salmon tissues. These two isoforms of EP4 receptor share high homology in their first half of sequence but differ in the second half part with several deletion segments though the final length of coding sequence is the same for two isoforms. We further studied the immunomodulation effect of PGE2 in TO cells and found that PGE2 inhibited the induction of CXCL-10, CCL-4, IL-8 and IL-1β genes expression in a time dependent manner and without cAMP upregulation. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 4 e0120483
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tz Chun Guo
Amr Ahmed Abdelrahim Gamil
Melanie Koenig
Øystein Evensen
Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
topic_facet Medicine
R
Science
Q
description PGE2 plays an important role in a broad spectrum of physiological and pathological processes mediated through a membrane-bound G protein-coupled receptor (GPCR) called EP receptor. In mammals, four subtypes of EP receptor (EP 1-4) are identified and each of them functions through different signal transduction pathways. Orthologous EP receptors have also been identified in other non-mammalian species, such as chicken and zebrafish. EP4 is the only identified PGE2 receptor to date in Atlantic salmon but its tissue distribution and function have not been studied in any detail. In this study, we first sequenced EP4 receptor in different tissues and found that the presence of the 3nt deletion in the 5' untranslated region was accompanied by silent mutation at nt 668. While attempting to amplify the same sequence in TO cells (an Atlantic salmon macrophage-like cell line), we failed to obtain the full-length product. Further investigation revealed different isoform of EP4 receptor in TO cells and we subsequently documented its presence in different Atlantic salmon tissues. These two isoforms of EP4 receptor share high homology in their first half of sequence but differ in the second half part with several deletion segments though the final length of coding sequence is the same for two isoforms. We further studied the immunomodulation effect of PGE2 in TO cells and found that PGE2 inhibited the induction of CXCL-10, CCL-4, IL-8 and IL-1β genes expression in a time dependent manner and without cAMP upregulation.
format Article in Journal/Newspaper
author Tz Chun Guo
Amr Ahmed Abdelrahim Gamil
Melanie Koenig
Øystein Evensen
author_facet Tz Chun Guo
Amr Ahmed Abdelrahim Gamil
Melanie Koenig
Øystein Evensen
author_sort Tz Chun Guo
title Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
title_short Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
title_full Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
title_fullStr Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
title_full_unstemmed Sequence analysis and identification of new isoform of EP4 receptors in different atlantic salmon tissues (Salmo salar L.) and its role in PGE2 induced immunomodulation in vitro.
title_sort sequence analysis and identification of new isoform of ep4 receptors in different atlantic salmon tissues (salmo salar l.) and its role in pge2 induced immunomodulation in vitro.
publisher Public Library of Science (PLoS)
publishDate 2015
url https://doi.org/10.1371/journal.pone.0120483
https://doaj.org/article/251bcd2b4d9f492a960d0214c10c865e
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source PLoS ONE, Vol 10, Iss 3, p e0120483 (2015)
op_relation http://europepmc.org/articles/PMC4383505?pdf=render
https://doaj.org/toc/1932-6203
1932-6203
doi:10.1371/journal.pone.0120483
https://doaj.org/article/251bcd2b4d9f492a960d0214c10c865e
op_doi https://doi.org/10.1371/journal.pone.0120483
container_title PLOS ONE
container_volume 10
container_issue 4
container_start_page e0120483
_version_ 1766360966581190656