Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans
Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarct...
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ftdoajarticles:oai:doaj.org/article:24eeed0268814e818badc347dc07f49c 2023-05-15T13:56:57+02:00 Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Zhaohui Zhang Linlin Zhao 2022-09-01T00:00:00Z https://doi.org/10.3389/fpls.2022.1006991 https://doaj.org/article/24eeed0268814e818badc347dc07f49c EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fpls.2022.1006991/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.1006991 https://doaj.org/article/24eeed0268814e818badc347dc07f49c Frontiers in Plant Science, Vol 13 (2022) cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes Plant culture SB1-1110 article 2022 ftdoajarticles https://doi.org/10.3389/fpls.2022.1006991 2022-12-30T22:01:22Z Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Plant Science 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes Plant culture SB1-1110 |
spellingShingle |
cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes Plant culture SB1-1110 Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Zhaohui Zhang Linlin Zhao Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
topic_facet |
cold stress flavonoids very-long-chain fatty acids metabolomic profiling transcriptomic sequencing bryophytes Plant culture SB1-1110 |
description |
Antarctica is the coldest, driest, and most windy continent on earth. The major terrestrial vegetation consists of cryptogams (mosses and lichens) and two vascular plant species. However, the molecular mechanism of cold tolerance and relevant regulatory networks were largely unknown in these Antarctic plants. Here, we investigated the global alterations in metabolites and regulatory pathways of an Antarctic moss (Pohlia nutans) under cold stress using an integrated multi-omics approach. We found that proline content and several antioxidant enzyme activities were significantly increased in P. nutans under cold stress, but the contents of chlorophyll and total flavonoids were markedly decreased. A total of 559 metabolites were detected using ultra high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). We observed 39 and 71 differentially changed metabolites (DCMs) after 24 h and 60 h cold stress, indicating that several major pathways were differentially activated for producing fatty acids, alkaloids, flavonoids, terpenoids, and phenolic acids. In addition, the quantitative transcriptome sequencing was conducted to uncover the global transcriptional profiles of P. nutans under cold stress. The representative differentially expressed genes (DEGs) were identified and summarized to the function including Ca2+ signaling, ABA signaling, jasmonate signaling, fatty acids biosynthesis, flavonoid biosynthesis, and other biological processes. The integrated dataset analyses of metabolome and transcriptome revealed that jasmonate signaling, auxin signaling, very-long-chain fatty acids and flavonoid biosynthesis pathways might contribute to P. nutans acclimating to cold stress. Overall, these observations provide insight into Antarctic moss adaptations to polar habitats and the impact of global climate change on Antarctic plants. |
format |
Article in Journal/Newspaper |
author |
Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Zhaohui Zhang Linlin Zhao |
author_facet |
Shenghao Liu Tingting Li Shuo Fang Pengying Zhang Dan Yi Bailin Cong Zhaohui Zhang Linlin Zhao |
author_sort |
Shenghao Liu |
title |
Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
title_short |
Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
title_full |
Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
title_fullStr |
Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
title_full_unstemmed |
Metabolic profiling and gene expression analyses provide insights into cold adaptation of an Antarctic moss Pohlia nutans |
title_sort |
metabolic profiling and gene expression analyses provide insights into cold adaptation of an antarctic moss pohlia nutans |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.1006991 https://doaj.org/article/24eeed0268814e818badc347dc07f49c |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Plant Science, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fpls.2022.1006991/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.1006991 https://doaj.org/article/24eeed0268814e818badc347dc07f49c |
op_doi |
https://doi.org/10.3389/fpls.2022.1006991 |
container_title |
Frontiers in Plant Science |
container_volume |
13 |
_version_ |
1766264548484972544 |