The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia
Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin bl...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2001
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ftdoajarticles:oai:doaj.org/article:24dbacd375684149acd1f3d6f73f229d 2023-05-15T15:09:20+02:00 The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia Alex Mutani James Stwart Kaminjolo 2001-01-01T00:00:00Z https://doi.org/10.1590/S0037-86822001000400012 https://doaj.org/article/24dbacd375684149acd1f3d6f73f229d EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400012 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822001000400012 0037-8682 1678-9849 https://doaj.org/article/24dbacd375684149acd1f3d6f73f229d Revista da Sociedade Brasileira de Medicina Tropical, Vol 34, Iss 4, Pp 377-380 (2001) Ehrlichia Cultura celular Células mononucleares Cell Culture Mononuclear cells Arctic medicine. Tropical medicine RC955-962 article 2001 ftdoajarticles https://doi.org/10.1590/S0037-86822001000400012 2022-12-30T23:06:02Z Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin blood smears from the same animals showed that only two were positive for Ehrlichia. The results of this investigation show that leukocyte culture is superior to the traditional thin blood film method in the detection of Ehrlichia and that asymptomatic carriers are easily detected. The method is inexpensive and does not require specific cell lines although it is necessary to use sterile sera. Leucócitos do sangue periférico de diferentes animais foram isolados do sangue total e mantidos em meio de Dulbeco, contendo soro homólogo sem antibióticos. Após 72 horas, um exame microscópico destas células mostrou que a maioria dos animais era infectada com Ehrlichia. Observação de esfregaços de sangue dos mesmos animais mostrou que apenas dois eram positivos para Ehrlichia. Os resultados desta pesquisa mostraram que a cultura de leucócitos é superior ao método tradicional de película de sangue na detecção de Ehrlichia , e que portadores assintomáticos são facilmente detectados. O método é de baixo custo e não exige linhas de células específicas, embora seja necessário o uso de soro estéril. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 34 4 377 380 |
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ftdoajarticles |
language |
English |
topic |
Ehrlichia Cultura celular Células mononucleares Cell Culture Mononuclear cells Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Ehrlichia Cultura celular Células mononucleares Cell Culture Mononuclear cells Arctic medicine. Tropical medicine RC955-962 Alex Mutani James Stwart Kaminjolo The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
topic_facet |
Ehrlichia Cultura celular Células mononucleares Cell Culture Mononuclear cells Arctic medicine. Tropical medicine RC955-962 |
description |
Peripheral blood leukocytes from different animals were isolated from whole blood and maintained in Dulbeco's medium containing homologous serum without antibiotics. After 72 hrs microscopic examination of these cells showed that most animals were infected with Ehrlichia. Observation of thin blood smears from the same animals showed that only two were positive for Ehrlichia. The results of this investigation show that leukocyte culture is superior to the traditional thin blood film method in the detection of Ehrlichia and that asymptomatic carriers are easily detected. The method is inexpensive and does not require specific cell lines although it is necessary to use sterile sera. Leucócitos do sangue periférico de diferentes animais foram isolados do sangue total e mantidos em meio de Dulbeco, contendo soro homólogo sem antibióticos. Após 72 horas, um exame microscópico destas células mostrou que a maioria dos animais era infectada com Ehrlichia. Observação de esfregaços de sangue dos mesmos animais mostrou que apenas dois eram positivos para Ehrlichia. Os resultados desta pesquisa mostraram que a cultura de leucócitos é superior ao método tradicional de película de sangue na detecção de Ehrlichia , e que portadores assintomáticos são facilmente detectados. O método é de baixo custo e não exige linhas de células específicas, embora seja necessário o uso de soro estéril. |
format |
Article in Journal/Newspaper |
author |
Alex Mutani James Stwart Kaminjolo |
author_facet |
Alex Mutani James Stwart Kaminjolo |
author_sort |
Alex Mutani |
title |
The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
title_short |
The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
title_full |
The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
title_fullStr |
The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
title_full_unstemmed |
The value of in vitro cell culture of granulocytes in the detection of Ehrlichia Valor da cultura celular de granulócito in vitro na detecção de Ehrlichia |
title_sort |
value of in vitro cell culture of granulocytes in the detection of ehrlichia valor da cultura celular de granulócito in vitro na detecção de ehrlichia |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2001 |
url |
https://doi.org/10.1590/S0037-86822001000400012 https://doaj.org/article/24dbacd375684149acd1f3d6f73f229d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 34, Iss 4, Pp 377-380 (2001) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000400012 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 doi:10.1590/S0037-86822001000400012 0037-8682 1678-9849 https://doaj.org/article/24dbacd375684149acd1f3d6f73f229d |
op_doi |
https://doi.org/10.1590/S0037-86822001000400012 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
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34 |
container_issue |
4 |
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377 |
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380 |
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